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Histological grade xylenes

Manufactured by Spectrum Chemical
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Histological-grade xylenes are solvents commonly used in histology and tissue processing applications. They serve as clearing agents to remove alcohol and prepare samples for embedding in paraffin. Histological-grade xylenes meet specific purity and quality standards required for these applications.

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Lab products found in correlation

Ethanol, by Merck Group (1 mentions) Methanol, by Merck Group (1 mentions) Acetonitrile, by Merck Group (1 mentions) Recombinant isoamylase, by Merck Group (1 mentions) Citraconic anhydride for antigen retrieval, by Thermo Fisher Scientific (1 mentions)

2 protocols using histological grade xylenes

1

MALDI-TOF Tissue Preparation Protocol

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MALDI matrix 1,5-diaminonaphthalene (1,5-DAN, ≥ 97%), tissue-embedding media gelatin from bovine skin (type B), sodium carboxymethyl cellulose (CMC), isopentane (≥95%), acetonitrile, ethanol, methanol, and water for H&E staining were purchased from Sigma Aldrich (St Louis, MO). Histological-grade xylenes were purchased from Spectrum Chemical. Hematoxylin and eosin were purchased from Cancer Diagnostics, Inc. and FisherBrand (Pittsburgh, PA), respectively. All chemicals were used as received.
Ma X., Botros A., Yun S.R., Park E.Y., Kim O., Park S., Pham T.H., Chen R., Palaniappan M., Matzuk M.M., Kim J, & Fernández F.M. (2024). Ultrahigh resolution lipid mass spectrometry imaging of high-grade serous ovarian cancer mouse models. Frontiers in Chemistry, 11, 1332816.
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2

Proteomic Sample Preparation Protocol

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High-performance liquid chromatography-grade acetonitrile, ethanol, methanol, water, and trifluoroacetic acid (TFA) were purchased from Sigma-Aldrich. α-cyano-4-hydroxycinnamic acid (CHCA) matrix was purchased from Cayman Chemical. Histological-grade xylenes was purchased from Spectrum Chemical. Citraconic anhydride for antigen retrieval was obtained from Thermo Fisher Scientific. Recombinant PNGaseF Prime was obtained from N-Zyme Scientifics (Doylestown, PA, USA), and recombinant isoamylase was obtained from Sigma-Aldrich.
Conroy L.R., Chang J.E., Sun Q., Clarke H.A., Buoncristiani M.D., Young L.E., McDonald R.J., Liu J., Gentry M.S., Allison D.B, & Sun R.C. (2022). High-dimensionality reduction clustering of complex carbohydrates to study lung cancer metabolic heterogeneity. Advances in cancer research, 154, 227-251.
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