1 2 dipalmitoyl sn glycero 3 phosphatidylinositol

High-performance liquid chromatography (HPLC)-grade dichloromethane (CH₂Cl₂), absolute ethanol 96% (CH₃CH₂OH), and methanol (CH₃OH) were obtained from Fisher Scientific Ltd. (Loughborough, UK). Milli-Q purified water (Synergy1, Millipore Corporation, Billerica, MA, USA) was used in all experiments. The 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS^●+) was purchased from Fluka (Buchs, Switzerland) and the α,α-diphenyl-β-pic-rylhydrazyl radical (DPPH^●) from Aldrich (Milwaukee, WI, USA). All the remaining reagents were obtained from primary commercial sources. The lipid internal standards used in the lipidome analyses were acquired from Avanti Polar Lipids, Inc. (Alabaster, AL, USA): 1,2-dimyristoyl-sn-glycero-3-phosphate (dMPA), 1,2-dimyristoyl-sn-glycero-3-phospho-(10-rac-glycerol) (dMPG), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (dMPC), 1,2-dipalmitoyl-sn-glycero-3-phosphatidylinositol (dPPI), 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (dMPE), 1,2-dimyristoyl-sn-glycero-3-phosphatidylserine (dMPS), 1-nonadecanoyl-2-hydroxy-sn-glycero-3-phosphocholine (LPC), N-heptadecanoyl-D-erythro-sphingosine (Cer), and 1′,3′-bis-[1-2-di-tetradecanoyl-sn-glycero-3-phospho]-sn-glycerol (CL), and N-heptadecanoyl-D-erythro-sphingosylphosphorylcholine (SM).

HPLC-grade chloroform (CHCl₃), methanol (MeOH), dichloromethane (CH₂Cl₂), and ethanol (EtOH, 96%) were purchased from Fisher Scientific Ltd. (Loughborough, UK). All other reagents were purchased from major commercial sources and used without further purification. (±) 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox) was purchased from Sigma-Aldrich (St Louis, MO, USA). 2,2-diphenyl-1-picrylhydrazy radical (DPPH^●) was purchased from Aldrich (Milwaukee, WI). 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS^●+) was obtained from Fluka (Buchs, Switzerland). 1,2-dimyristoyl-sn-glycero-3-phosphocholine (dMPC), 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (dMPE), N-heptadecanoyl-D-erythro-sphingosyl-phosphorylcholine (SM d18:1/17:0), 1-nonadecanoyl-2-hydroxy-sn-glycero-3-phosphocholine (LPC), 1,2-dipalmitoyl-sn-glycero-3-phosphatidylinositol (dPPI), 1,2-dimyristoyl-sn-glycero-3-phospho-(10-rac-) glycerol (dMPG), 1,2-dimyristoyl-sn-glycero-3-phospho-L-serine (dMPS), 1,2-dimyristoyl-sn-glycero-3-phosphate (dMPA), and C17 Ceramide (d35:1) were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL). Milli-Q water (Synergy, Millipore Corporation, Billerica, MA, USA) was used.

Dichloromethane (CH₂Cl₂), methanol (MeOH), and acetonitrile (high-performance liquid chromatography—HPLC, grade) were purchased from Fisher Scientific Ltd. (Loughborough, UK). Milli-Q water (Synergy, Millipore Corporation, Billerica, MA, USA) was used. Phospholipid standards: 1,2-dimyristoyl-sn-glycero-3- phosphocholine (dMPC), 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (dMPE), 1,2-dimyristoyl-sn-glycero-3-phospho-(1′-rac-)glycerol (dMPG), 1,2-dipalmitoyl-sn-glycero-3-phosphatidylinositol (dPPI), 1–nonadecanoyl-2-hydroxy-sn-glycero-3-phosphocholine (LPC), dimyristoyl phosphatidic acid (dMPA), and N-heptadecanoyl-D-erythro-sphingosine (Cer (d18:1/17:0)) were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL).
2,2-Diphenyl-1-picrylhydrazyl (DPPH) was purchased from Aldrich (Milwaukee, WI). 2,2’-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) was obtained from Fluka (Buchs, Switzerland). Ammonium acetate and 6-hydroxyl-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox) were purchased from Sigma-Aldrich (St Louis, MO, USA). All other reagents and chemicals used were of the highest grade of purity and were purchased from major commercial sources.