Pseudomanas aeruginosa, VRSA, MSSA and MRSE strains were obtained from the American Type Culture Collection (ATCC 700699, ATCC10145, ATCC 25,923 and ATCC 51,625, respectively; Manassas, VA, USA). A. baumannii and MRSA strains were obtained from the culture collection of the Göteburg University (CCUG; Gothenburg, Sweden) (CCUG 61,012 and CCUG 60578). S. epidermidis was obtained from the Belgian Co-ordinated Collections of Microorganisms (Belgium) (LMG 10474; Gent, Belgium).
For antimicrobial testing inoculums were prepared in Luria-Bertani Broth (LB; ThermoFisher Scientific, Waltham, MA, USA) and for biofilms assays in Tryptic Soy Broth (TSB, Biokar Diagnostics, Beauvais, France).
For cell-based assays human keratinocytes (HaCat) were obtained from Cell Line Services (Oppenheim, Denmark). For assays cells were cultured, at 37 °C in a humidified atmosphere of 95% air and 5% CO2, using Dulbecco’s Modified Eagle’s Medium (DMEM) with 4.5 g/L glucose, L-glutamine without pyruvate (Lonza, Verviers, Belgium) containing 10% foetal bovine serum (FBS, Biowest, Nuaillé, France) and 1% (v/v) Penicillin-Streptomycin-Fungizone (Lonza, Verviers, Belgium).
For antimicrobial testing inoculums were prepared in Luria-Bertani Broth (LB; ThermoFisher Scientific, Waltham, MA, USA) and for biofilms assays in Tryptic Soy Broth (TSB, Biokar Diagnostics, Beauvais, France).
For cell-based assays human keratinocytes (HaCat) were obtained from Cell Line Services (Oppenheim, Denmark). For assays cells were cultured, at 37 °C in a humidified atmosphere of 95% air and 5% CO2, using Dulbecco’s Modified Eagle’s Medium (DMEM) with 4.5 g/L glucose, L-glutamine without pyruvate (Lonza, Verviers, Belgium) containing 10% foetal bovine serum (FBS, Biowest, Nuaillé, France) and 1% (v/v) Penicillin-Streptomycin-Fungizone (Lonza, Verviers, Belgium).