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Fluorescent probes and secondary antibodies coupled to fluorescent markers

Manufactured by Thermo Fisher Scientific

Fluorescent probes and secondary antibodies coupled to fluorescent markers are laboratory reagents used to detect and visualize target molecules in various biological applications. These products provide a means to label and identify specific proteins, cells, or other biomolecules through fluorescent signal detection.

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2 protocols using fluorescent probes and secondary antibodies coupled to fluorescent markers

1

Cellular Stress Response Assay

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d-galactose (G0750), 4-phenylbutyric acid (4-PBA, P21005), and tunicamycin (T7765) were purchased from Sigma Aldrich (St Louis, MO, USA). GKT137831 were purchased from Cayman Chemical (1218942-37-0, Ann Arbor, MI, USA). Cell culture media and antibiotics were obtained from Lonza and Gibco. Fluorescent probes and secondary antibodies coupled to fluorescent markers were purchased from Molecular Probes, Invitrogen. All other reagents were obtained from Sigma or the highest grade available.
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2

Investigating Cellular Stress Pathways

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Etoposide, doxorubicin, 5-fluorouracil, hidroxyurea, imatinib and 4μ8C were purchased from Sigma Aldrich. Tunicamycin was obtained from Calbiochem EMB Bioscience Inc. IRE1 inhibitor MKC-8866 was provided by Dr. John Patterson (Fosun Orinove). Cell culture media, fetal calf serum, and antibiotics were obtained from Life Technologies and Sigma Aldrich. Fluorescent probes and secondary antibodies coupled to fluorescent markers were purchased from Molecular Probes, Invitrogen. All other reagents were obtained from Sigma or the highest grade available.
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