Nod scid il2 receptor gamma chain knockout nsg mice

Manufactured by Jackson ImmunoResearch

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NSG mice are a strain of immunodeficient mice that lack mature T cells, B cells, and natural killer cells. They have a targeted mutation in the IL2 receptor gamma chain, which is essential for the development of these cell types. As a result, NSG mice are highly susceptible to engraftment of human cells and tissues, making them a valuable model for studying human diseases and evaluating the efficacy of therapeutic interventions.

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Lab products found in correlation

Donkey anti rabbit fitc, by Abcam (1 mentions) Goat anti dsred antibody, by Santa Cruz Biotechnology (1 mentions) Donkey anti goat pe, by Abcam (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Triton x 100, by MP Biomedicals (1 mentions) Donkey serum, by Biosynth (1 mentions) Matrigel matrix, by Corning (1 mentions)

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NSG mice (401 citations) NOD/SCID mice (263 citations) NOD scid gamma (NSG) mice (168 citations) NSG (123 citations) NOD/SCID (81 citations) SCID mice (40 citations) NOD/SCID gamma mice (NSG; (6 citations) NOD SCID gamma chain knockout (NSG) mice (2 citations) NOD Scid gamma (NSG; NOD-SCID) mice (2 citations) NSG (NOD-SCID) mice (2 citations)

4 protocols using nod scid il2 receptor gamma chain knockout nsg mice

Teratoma Formation and Analysis

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Severely immune-compromised NOD/SCID IL2 receptor gamma chain knockout (NSG) mice (Jackson Laboratories) were injected with 1 million ESC in 100 μL of PBS subcutaneously in the dorsal flanks of each mouse using a 28 gauge needle. After 30 days, the mice were euthanized by cervical dislocation and the teratomas harvested and sent out for histological processing (IDEXX Laboratories). The prepared slides were then dehydrated in graded alcohol, de-paraffinized using three washes of xylene, then rehydrated in graded alcohol and placed in PBS until imaging with a confocal microscope. After deparaffinization, the GFP and RFP were either directly visualized, or counterstained in 1% BSA (Sigma), 5% donkey serum (Fitzgerald), 0.7% Triton X-100 (MP Biomedical) followed by a goat anti-DsRed antibody (Santa Cruz, 1:50 dilution) followed by donkey-anti goat PE (Abcam, 1:100 dilution). DAPI (4′,6-Diamidino-2-Phenylindole, Dilactate) was used to visualize the nuclei and images were taken on a Nikon TE 2000 fluorescent microscope. To verify endothelial and smooth muscle cell identity, goat anti-VE (vascular endothelial)-cadherin (Santa Cruz, 1:100) with a donkey anti-goat (Santa Cruz, 1:100) or rabbit anti-smooth muscle myosin heavy chain (Abcam, 1:50) with a donkey anti-rabbit FITC (Abcam, 1:50) was also used in conjunction with the direct visualization of the GFP or the counter stained RFP.

Glaser D.E., Burns A.B., Hatano R., Medrzycki M., Fan Y, & McCloskey K.E. (2014). Specialized mouse embryonic stem cells for studying vascular development. Stem Cells and Cloning : Advances and Applications, 7, 79-88.

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Nicotine Impact on Pancreatic Cancer Xenografts

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All animal studies were performed with approval from the University of Florida Institutional Animal Care and Use Committee. A 2×2 mm section of a surgically resected primary pancreatic adenocarcinoma or 2 mm core biopsy was implanted subcutaneously into 8-week-old female NOD-SCID IL2 receptor gamma chain knockout (NSG) mice (Jackson Laboratory, Bar Harbor, ME) (n = 20 mice). Mice were anesthetized using inhaled isofluorane during the procedure and administered two doses of buprenorphine immediately and 12 hours postoperatively. On post-operative day 5, mice with visible tumors were equally randomized by size to receive 1 mg/kg nicotine (n = 10) or an equal volume of PBS (n = 10) three times per week via intraperitoneal injections as previously described (12 (link)). Tumor dimensions were measured three times per week using calipers. Tumor volumes were calculated using the equation: v = xy²/2 where v is volume, x is tumor length and y is tumor width. Tumors were allowed to reach an endpoint of 2 cm in maximum diameter prior to euthanasia. Primary tumors and lungs were harvested for immunohistochemical analysis.

Delitto D., Zhang D., Han S., Black B.S., Knowlton A.E., Vlada A.C., Sarosi G.A., Behrns K.E., Thomas R.M., Lu X., Liu C., George T.J., Hughes S.J., Wallet S.M, & Trevino J.G. (2015). Nicotine Reduces Survival via Augmentation of Paracrine HGF-MET Signaling in the Pancreatic Cancer Microenvironment. Clinical cancer research : an official journal of the American Association for Cancer Research, 22(7), 1787-1799.

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Xenograft Tumor Models in NSG Mice

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All animal studies were performed with approval from the University of Florida Institutional Animal Care and Use Committee. Flank tumors were engrafted by subcutaneous injection of 10⁶ cancer cells embedded in 200 μL of 50% Matrigel® matrix (Corning, NY) into 8-week-old female NOD-SCID IL2 receptor gamma chain knockout (NSG) mice (Jackson Laboratory, Bar Harbor, ME). Alternatively, orthotopic injections were performed using 10⁶ pancreatic cancer cells embedded in 50 μL of 50% Matrigel® into the pancreas using a surgical technique described previously [40 (link)]. Control groups consisted of equal volumes of 50% Matrigel® matrix injected into age- and gender-matched NSG mice. Mice were anesthetized using inhaled isoflurane during the procedure and administered two doses of buprenorphine immediately and 12 hours postoperatively. Flank tumors were allowed to reach an endpoint of 2 cm in maximum diameter prior to euthanasia. Mice with orthotopic xenografts were euthanized when palpable intra-abdominal tumors reached 1 cm in size.

Delitto D., Judge S.M., Delitto A.E., Nosacka R.L., Rocha F.G., DiVita B.B., Gerber M.H., George TJ J.r., Behrns K.E., Hughes S.J., Wallet S.M., Judge A.R, & Trevino J.G. (2016). Human pancreatic cancer xenografts recapitulate key aspects of cancer cachexia. Oncotarget, 8(1), 1177-1189.

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NSG Mouse Maintenance and Evaluation

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NOD scid IL2 receptor gamma chain knockout (NSG) mice were purchased from The Jackson Laboratory and maintained at the University of Minnesota under specific pathogen-free conditions utilizing protocols approved by the Institutional Animal Care and Use Committee. Mice were treated and assessed at different harvest times as described.

Felices M., Lenvik T.R., Ankarlo D.E., Foley B., Curtsinger J., Luo X., Blazar B.R., Anderson S.K, & Miller J.S. (2014). Functional NK cell repertoires are maintained through IL-2Rα and FasL. Journal of immunology (Baltimore, Md. : 1950), 192(8), 3889-3897.

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