For the tumor xenograft mouse model construction, 15 BALB/c nude mice (4–6 weeks old; 18–20 g) were purchased from Beijing HFK Bioscience Co., Ltd. (Beijing, China). Animals were maintained in specific pathogen-free conditions and an environment with controlled conditions of light and humidity, and free access to water and a standard laboratory diet. Animal experiments were performed in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals (Bethesda, MD, USA). The present study was approved by the ethics committee of the Scientific Investigation Board of Science and Technology of Jilin Province (Changchun, China). Mice were randomly divided into four groups (five animals per group), including the SMMC-7721 group, the NC group, the MR1-D4 group and the MR1-D9 group. Cells (2×106) were subcutaneously injected into the right flank of each mouse. For real-time near-infrared (NIR) imaging, the mice were anesthetized and imaged on day 21 post-injection, and in vivo NIR images were obtained using a Xenogen IVIS Spectrum system (PerkinElmer, Inc., Waltham, MA, USA) using Living Image software version 3.0 (Caliper Life Sciences, Alameda, CA, USA). All image analysis and NIR fluorescent signal quantification was performed using the region of interest (ROI) function of Living Image software (Caliper Life Sciences).
Xenogen ivis spectrum system
Manufactured by PerkinElmer
Sourced in United States
The Xenogen IVIS Spectrum system is a high-performance, in vivo imaging system designed for preclinical research. It provides quantitative, non-invasive, and real-time visualization of biological processes in living small animals. The system utilizes a sensitive charge-coupled device (CCD) camera to detect bioluminescent and fluorescent signals from labeled cells or reporter genes.
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Lab products found in correlation
3 protocols using xenogen ivis spectrum system
1
Tumor Xenograft Mouse Model Construction
2
In Vivo Cell Labeling and Imaging
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A total of 1.0 × 106 cells was labeled with XenoLight DiR (Perkin Elmer, Waltham, MA, USA). The staining procedure was performed according to the manufacturer’s instructions. Briefly, cells were treated with DiR for 30 min at 37 °C, centrifuged for 3 min at 400 × g at room temperature, and washed twice with PBS. In all cases, DiR-labeled cells were suspended in saline and then intravenously injected through the tail vein within 2 h of labeling. Before taking the fluorescence images, the hair of C57BL/6 mice was removed to avoid any interference in detecting the fluorescence signal. Fluorescence images were obtained at 1, 2, 4, and 6 h after injection using the Xenogen IVIS Spectrum system (Perkin Elmer). All images were acquired with excitation at 748 nm and emission at 780 nm.
3
Xenograft and Intracranial Mouse Models
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All experimental animal procedures were conducted strictly by the Guide for the Care and Use of Laboratory Animals and approved by the Animal Care and Use Committee of the Shandong provincial hospital affiliated to Shandong University. The sample size of the animal experiment was based on the preliminary experiments and similar well-designed experiments, and no statistical method was used. The male BALB/c nude mice were randomized divide into two groups, each group including six 4 weeks old nude mice. Investigators were blinded to the treatment groups during data collection and subsequent data analysis. In the subcutaneous xenograft model, 5 × 105 cells were subcutaneously injected in the right flanks of nude mice. In the orthotopic intracranial mouse model, each mouse was intracranially injected with 1 × 105 luciferase transfected U87MG cells in 10 μL PBS solution. Tumor growth was monitored by a Xenogen IVIS Spectrum system (PerkinElmer).
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