After the rats were sacrificed, the injured aortic tissues were fixed with 4% paraformaldehyde solution, embedded in paraffin, and cut into 6 μm sections, which were prepared and incubated with rabbit anti-CD31 (Servicebio, China) overnight at 4 °C. After routine immunohistochemistry, the sections were observed under a microscope (Olympus, Tokyo, Japan). Image-Pro Plus 6.0 software was used to calculate the ratio of CD31-expressing cells to the total number of cells in the arterial lumen wall to measure the re-endothelialization rate for each cross-section.
Rabbit anti cd31
Manufactured by Wuhan Servicebio Technology
Sourced in China
Rabbit anti-CD31 is a primary antibody that recognizes the CD31 protein, also known as platelet endothelial cell adhesion molecule (PECAM-1). CD31 is a transmembrane glycoprotein expressed on the surface of endothelial cells, platelets, and certain immune cells. This antibody can be used for the detection and analysis of CD31-positive cells in various research applications.
Automatically generated - may contain errors
Lab products found in correlation
4 6 diamidino 2 phenylindole (dapi), by Wuhan Servicebio Technology (1 mentions)
Cy3 conjugated goat anti rabbit igg, by Wuhan Servicebio Technology (1 mentions)
Mouse anti α sma, by Wuhan Servicebio Technology (1 mentions)
Vs120 s6 w, by Olympus (1 mentions)
Confocal laser scanning microscopy, by Nikon (1 mentions)
Mouse anti brdu, by Merck Group (1 mentions)
Mouse anti neun, by Wuhan Servicebio Technology (1 mentions)
Rabbit anti nestin, by Santa Cruz Biotechnology (1 mentions)
4 protocols using rabbit anti cd31
1
Quantifying Aortic Re-endothelialization in Rats
2
Immunofluorescent Analysis of Angiogenesis
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Briefly, after deparaffinization, hydration, and antigen retrieval, the skin tissue sections were blocked with 5% BSA at room temperature for 1 h and then incubated with the primary antibodies rabbit anti-CD31(1:300, Servicebio, Wuhan, China) and mouse anti-α-SMA (1:200, Servicebio) (dissolved in 1% BSA) at 4 °C overnight. After washing with PBS, the sections were incubated with Cy3-conjugated goat anti-rabbit IgG (1:300, Servicebio) and Alexa Flour 488-conjugated goat anti-mouse IgG (1:400, Servicebio) at room temperature for 1 h. Afterward, DAPI (Servicebio) was added dropwise and incubated for 10 min. Finally, sections were captured by the virtual slide microscope (VS120-S6-W, OLYMPUS), and the analysis of CD31 and α-SMA positive staining cells was quantified using ImageJ software.
3
Histological Evaluation of Angiogenesis
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All skin specimens were dehydrated and subsequently embedded in paraffin. For bone specimens, a decalcification process was added before dehydration. After sectioning the tissues, hematoxylin & eosin (HE) staining or Masson staining was performed. For angiogenesis analysis, immunohistochemical and immunofluorescence staining were carried out with the following primary antibodies: rabbit anti-CD31 (1:2000, Servicebio, China) and rabbit anti-αSMA (1:2000, Boster Biological Technology, USA). Target fields from each staining sample were recorded.
4
Histological Analysis of Brain Tissue Post-Cell Transplantation
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Two days after cell transplantation, the head of the rats was severed, and the left brain tissue was removed and fixed in 4% paraformaldehyde for 1–3 days. For dehydration, 30% sucrose paraformaldehyde was applied. Paraffin sections, 4-μm thick, were prepared, and morphological and histopathological observations were carried out under a light microscope after HE staining. For double-immunofluorescence labeling, brain tissue was sectioned to 15 μm after dehydration, and these sections were immunostained with monoclonal mouse anti-BrdU (Sigma–Aldrich, USA), rabbit anti-Nestin (Santa Cruz, USA), rabbit anti-CD31 (Servicebio, China), and mouse anti-NeuN (Servicebio, China) antibodies at 4 °C for 20 h. The primary antibodies were visualized using Alexa Fluor 488 chicken anti-mouse IgG and Cy3-conjugated Affinipure goat anti-rabbit IgG. Laser scanning confocal microscopy (Nikon, Japan) was applied to capture images.
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