The metabolic stability of selected hits was tested using human, bovine, horse and mouse liver microsomes (Corning) of phase-I (CYP450 and NADPH dependent enzymes) and phase-II (UGT enzymes) metabolism as described before (Hulpia et al., 2019a (link)). In short, samples were collected after 0, 15, 30 and 60 min and the corresponding loss of parent compound was determined using ultra-performance liquid chromatography (UPLC) (Waters AquityTM) coupled with tandem quadrupole mass spectrometry (MS2) (Waters XevoTM), equipped with an electrospray ionization (ESI) interface and operated in multiple reaction monitoring (MRM) mode.
Xevo
Manufactured by Waters Corporation
The Xevo™ is a mass spectrometry instrument designed for analytical and research applications. It provides accurate and reliable mass measurements for the identification and quantification of various chemical compounds. The Xevo™ utilizes advanced ionization techniques and high-resolution mass analyzers to deliver precise and sensitive results.
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Lab products found in correlation
2 protocols using xevo
1
Evaluating Compound Metabolic Stability
2
Targeted UPLC-MS/MS for Compound Analysis
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Chromatography was performed on a Waters Acquity UPLC system coupled to a Waters Xevo TM dependent acquisition where the 8 most intense ions detected in the MS spectrum were selected as MS/MS precursors. Typically, the threshold for MS/MS targeting was set to 50 with targeted ions subsequently excluded from targeting for 15 seconds. MS/MS scans were obtained for selected ions with CID fragmentation, isolation width of 2.0, normalized collision energy of 36 V, activation Q of 0.250, and activation time of 30 ms.
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