Fluorescently labeled antibodies (Abs) to CD3 (145-2C11), CD25 (PC51), CD44 (IM7), CD16/CD32 (2.4G2), CD14 (rmC5-3), CD45R/B220 (RA3-6B2), and IgG2a isotype control were purchased from BD Biosciences (San Jose, CA). Fluorescently labeled Abs to GATA3 (TWAJ) and IgG1 isotype control were purchased from eBioscience (San Diego, CA). Fluorescently labeled Annexin-V, IgG2b isotype control, and 7-AAD viability staining solution were purchased from BioLegend (San Diego, CA). Ghost Dye™ Red 780 was purchased from TONBO Biosciences (San Diego, CA). Recombinant mouse proteins, including IL-2, IL-7, IL-25, TSLP, and IFN-β were from R&D Systems (Minneapolis, MN). Mouse IL-33 and IFN-α2 were from eBioscience, and mouse IFN-γ was from PeproTech (Rocky Hill, NJ). High molecular weight poly (I:C), CpG A (ODN1585), and R848 were from InvivoGen (San Diego, CA). The culture filtrate extract of A. alternata was from Greer Laboratories (Lenoir, NC); the extract contained detectable, but minimal, amounts of endotoxin (i.e., 3 ng endotoxin/mg extract). Anti-IFNAR1 (MAR1-5A3) and isotype-matched control IgG for blocking experiments were purchased from BioXcell (West Lebanon, NH).
Igg2b isotype control
Manufactured by BioLegend
Sourced in United States
The IgG2b isotype control is a laboratory reagent used as a negative control in flow cytometry and other immunoassays. It serves as a reference to distinguish specific antibody binding from non-specific binding.
Automatically generated - may contain errors
Lab products found in correlation
Fluorescently labeled antibodies, by BD (1 mentions)
Mouse ifn γ, by Thermo Fisher Scientific (1 mentions)
Il 25, by R&D Systems (1 mentions)
7 aad viability staining solution, by BioLegend (1 mentions)
Ghost dye red 780, by Cytek Biosciences (1 mentions)
Anti ifnar1 mar1 5a3, by BioXCell (1 mentions)
Isotype matched control igg, by BioXCell (1 mentions)
Igg2a, by BD (1 mentions)
Interleukin 7 (il 7), by R&D Systems (1 mentions)
Interleukin 2 (il 2), by R&D Systems (1 mentions)
Ifn β, by R&D Systems (1 mentions)
Anti human cd44 antibody, by BioLegend (1 mentions)
Ethylene diamine tetraacetic acid (edta), by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by BioLegend (1 mentions)
Anti mouse ly6g antibody, by BioLegend (1 mentions)
4 protocols using igg2b isotype control
1
Murine Immune Cell Staining Protocol
2
Organoid Single-Cell Immunophenotyping
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Organoids were dissociated into single cells following the methods outlined above (see Human Organoid Passaging and Maintenance Culture in EHS Matrix and Enteroid Passaging in HELP Matrices). The cells were centrifuged for 5 min at 500 x g to pellet. The media was then removed from each pellet and the cells were resuspended in FACS buffer (PBS + 1 × 10−3m EDTA (Invitrogen) + 2% v/v FBS (Atlanta Bio) + 1% penicillin/streptomycin (Gibco)) supplemented with fluorophore‐conjugated primary antibodies (BioLegend anti‐human CD44 antibody, BioLegend IgG2B isotype control). Antibody staining was performed for 30 min at 4 °C in the dark. Following staining, the cells were washed twice using FACS buffer and resuspended in 200 µL FACS buffer with DAPI (1:10000, BioLegend) to select for live cells. Flow cytometry was performed on a Beckman Coulter CytoFlex analyzer (Stanford Stem Cell Institute FACS Core). To analyze the data, gates were determined using forward and side scatter with height and width used to identify cell doublets. Subsequently, live DAPI‐negative cells were gated for all marker analyses and population frequency calculations.
3
Modulating PD-L1 in Islet Xenografts
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To study the role of PD-L1 on islet xenograft survival, islet recipients were given two doses of 100 μl of PBS containing anti-mouse monoclonal anti–PD-L1 antibody (BioXCell, West Lebanon, NH, USA) or IgG2b isotype control (BioLegend, San Diego, CA, USA) at 2.5 mg/kg via intraperitoneal route on days 10 and 20 after transplantation.
4
Neutrophil Depletion for BSA-NP Study
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To deplete neutrophils, mice were treated with a 400 μg intraperitoneal injection of anti-mouse Ly6G antibody (108454, Biolegend) or IgG2b isotype control (400675, Biolegend) 24 h prior to injection of BSA-NPs. Subsequent 200 μg antibody injections were administered accompanied by BSA-NPs. Animals were euthanized after 16 h to collect peritoneal lavage fluid, ectopic lesions, and eutopic endometrium. Neutrophil depletion was confirmed by flow cytometry as described below.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!