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Ab6507

Manufactured by Abcam
Sourced in United Kingdom

Ab6507 is a monoclonal antibody that recognizes the Cytokeratin 18 protein. It is designed for use in immunohistochemistry applications.

Automatically generated - may contain errors

2 protocols using ab6507

1

Monoclonal Antibodies for HSV-1 and HSV-2

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Anti-HSV-1 + HSV-2 gB mouse monoclonal (ab6506), anti-HSV-1 ICP4 mouse monoclonal (ab6514), anti-HSV-1 ICP8 mouse monoclonal (ab20194), anti-HSV1 + HSV2 gD mouse monoclonal (ab6507), and anti-HSV-1 + HSV-2 ICP5 mouse monoclonal (ab6508) were purchased from Abcam (Cambridge, UK). Horseradish peroxidase (HRP)-conjugated anti-mouse IgG (SA00001-1, Proteintech, Wuhan, China) were used for immunoblotting, and goat anti-mouse 488 Alexa Fluor secondary antibody (4408S, CST, Danvers, MA, USA) was used for immuno-fluorescence. DAPI solution (C0060, Solarbio, Beijing, China) was used to stain the nuclei of the cells.
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2

Western Blot Analysis of GFP and HSV gD

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Samples were denatured in 5× loading buffer at 99 °C for 8 min, and separated by using 10% SDS-PAGE electrophoresis and then transferred onto polyvinylidene fluoride (PVDF) membranes. GFP proteins were detected using a rabbit anti-GFP antibody (ab290, Abcam) at a 1:2000 dilution. HSV gD proteins were detected using a mouse monoclonal antibody (ab6507, Abcam) at a 1:5000 dilution. A monoclonal antibody against β-tubulin (Proteintech) was used at a 1:8000 dilution. Secondary detection antibodies were used at a 1:5000 dilution. The images were collected and analyzed by gel-imaging (BioRad, ChemiDocTM MP).
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