The immunoblot analysis was performed using an enhanced chemiluminescence (ECL) system (Millipore, Billerica, MA, USA), and results were monitored using the LAS-4000 imaging system (Fujifilm, Tokyo, Japan). The primary antibodies, including polyclonal anti-RBM4 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), polyclonal anti-GAPDH (MDBio, Taipei, Taiwan), polyclonal anti-SP1 (Abcam, Taipei, Taiwan), monoclonal anti-SRPK1 (BD Transduction, San Jose, CA, USA), and monoclonal anti-MYC (MDBio) were applied in this study.
Polyclonal anti rbm4
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Polyclonal anti-RBM4 is a laboratory reagent used for the detection and analysis of the RBM4 protein in biological samples. RBM4 is an RNA-binding protein involved in various cellular processes. This antibody can be used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to study the expression and localization of RBM4 in cells and tissues.
Automatically generated - may contain errors
Lab products found in correlation
Ecl system, by Merck Group (3 mentions)
Las 4000 imaging system, by Fujifilm (3 mentions)
Monoclonal anti flag m2, by Merck Group (2 mentions)
Polyclonal anti erk1 2, by Cell Signaling Technology (2 mentions)
Monoclonal anti srpk1, by BD (1 mentions)
Monoclonal anti ptbp2, by Abnova (1 mentions)
Monoclonal anti nova1, by Abnova (1 mentions)
Monoclonal anti phospho akt, by Cell Signaling Technology (1 mentions)
3 protocols using polyclonal anti rbm4
1
Immunoblot Analysis of RBM4, SRPK1, and MYC
2
Quantitative Immunoblot Analysis Techniques
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The immunoblot analysis was conducted using an enhanced chemiluminescence (ECL) system (Millipore, Billerica, MA, USA), and images were analyzed with the LAS-4000 imaging system (Fujifilm, Tokyo, Japan). Primary antibodies used in this study included polyclonal anti-RBM4 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), monoclonal anti- PTBP2 (Abnova, Taipei, Taiwan), monoclonal anti-PTBP1 (EMD, Millipore), monoclonal anti-Nova1(Abnova), monoclonal anti-Actin (EMD, Millipore), monoclonal anti-FLAG M2 (Sigma-Aldrich, St. Louis, MO, USA), polyclonal anti-ERK1/2, and monoclonal anti-phospho-ERK1/2 (Cell Signaling Technology, Danvers, MA, USA). Intensities of the detected signals were determined using TotalLab Quant Software.
3
Immunoblot Analysis of Protein Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The immunoblot analysis was conducted using an enhanced chemiluminescence (ECL) system (Millipore, Billerica, MA, USA), and images were analyzed with the LAS-4000 imaging system (Fujifilm, Tokyo, Japan). Primary antibodies used in this study included polyclonal anti-RBM4 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), monoclonal anti-nPTB (Abnova, Taipei, Taiwan), polyclonal anti-GAPDH (MDBio, Taipei, Taiwan), monoclonal anti-FLAG M2 (Sigma-Aldrich, St. Louis, MO, USA), polyclonal anti-ERK1/2, polyclonal anti-AKT, monoclonal anti-phospho-ERK1/2 and monoclonal anti-phospho-AKT (Cell Signaling Technology, Danvers, MA, USA).
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