Mli 2

Manufactured by Abcam

MLi-2 is a lab instrument designed for molecular biology applications. It functions as a fluorescence microplate reader, capable of detecting and quantifying fluorescent signals in multi-well plates.

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Lab products found in correlation

Lrrk2 in 1, by Bio-Techne (2 mentions) Rock inhibitor y 27632, by Bio-Techne (1 mentions) Ethylene diamine tetraacetic acid (edta), by Thermo Fisher Scientific (1 mentions) Go6983, by Merck Group (1 mentions) Ap21967, by Takara Bio (1 mentions) Mitotracker red cmxros, by Cell Signaling Technology (1 mentions) Gsk2578215a, by Bio-Techne (1 mentions) Colchicine, by Merck Group (1 mentions) Poly 1 c, by InvivoGen (1 mentions) Recombinant human ifn γ, by R&D Systems (1 mentions) Recombinant human nrg1, by BioLegend (1 mentions) Mg132, by Merck Group (1 mentions) Ionomycin, by Merck Group (1 mentions) Phorbol myristate acetate (pma), by Merck Group (1 mentions) Lipopolysaccharides (lps), by InvivoGen (1 mentions)

3 protocols using mli 2

Protein Detection: Antibodies and Reagents

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A summary of the primary antibodies used in this study can be found in SI Appendix, Table S1. The following secondary antibodies conjugated to horseradish peroxidase were used for immunoblotting: RRID:AB_2099233, RRID:AB_330924, and RRID:AB_228395. Immunofluorescence experiments used the following secondary antibodies: RRID:AB_2535794 and RRID:AB_141637. The following chemicals were used: Y-27632 Rock inhibitor (Tocris Bioscience), MLi-2 (Abcam #254528), LRRK2-IN-1 (Tocris Bioscience # 4273), and EDTA (Invitrogen #15575-038).

Yadavalli N, & Ferguson S.M. (2023). LRRK2 suppresses lysosome degradative activity in macrophages and microglia through MiT-TFE transcription factor inhibition. Proceedings of the National Academy of Sciences of the United States of America, 120(31), e2303789120.

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Mitochondrial Dynamics Modulation Assay

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The following reagents were used at final concentrations as indicated: xhloroquine (CQ) (50 μM, Sigma-Aldrich), phorbol 12-myristate 13-acetate (PMA) (100 nM, Santa Cruz Bioctechnology), MLi-2 (50 nM, Abcam), Go6983 (200 nM, Sigma-Aldrich), AP21967 (1 μM, TaKaRa) and MitoTracker Red CMXRos (100 nM, Cell Signaling Technology).

Komori T., Kuwahara T., Fujimoto T., Sakurai M., Koyama-Honda I., Fukuda M, & Iwatsubo T. (2023). Phosphorylation of Rab29 at Ser185 regulates its localization and role in the lysosomal stress response in concert with LRRK2. Journal of Cell Science, 136(14), jcs261003.

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Stimulating Cell Responses with Diverse Agents

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Where indicated, cells were treated with recombinant human IFN-γ (R&D Systems), LPS (LPS from E. coli O111:B4), poly(I:C) (both Invivogen), ionomycin, MG132, colchicine, PMA (all from Sigma-Aldrich), LRRK2-IN-1 and GSK2578215A (both Tocris), Mli-2 (Abcam), MCV1 (HPVIVIT, Calbiochem), recombinant human NRG1 (BioLegend), and PTX (STEMCELL Technologies).

Panagiotakopoulou V., Ivanyuk D., De Cicco S., Haq W., Arsić A., Yu C., Messelodi D., Oldrati M., Schöndorf D.C., Perez M.J., Cassatella R.P., Jakobi M., Schneiderhan-Marra N., Gasser T., Nikić-Spiegel I, & Deleidi M. (2020). Interferon-γ signaling synergizes with LRRK2 in neurons and microglia derived from human induced pluripotent stem cells. Nature Communications, 11, 5163.

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