Anti tyrosinase antibody

Manufactured by Santa Cruz Biotechnology

Sourced in United States

The Anti-tyrosinase antibody is a laboratory reagent used for the detection and quantification of tyrosinase, an enzyme involved in the production of melanin. This antibody can be utilized in techniques such as Western blotting, immunohistochemistry, and ELISA to study the expression and localization of tyrosinase in various biological samples.

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Intissue tek o c t compound, by Sakura Finetek (1 mentions)

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Tyrosinase (24 citations) Anti-tyrosinase (10 citations)

2 protocols using anti tyrosinase antibody

Effects of Peptides on Melanocyte Tyrosinase Expression

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Example 4

Melanocytes (B16F10 cell line) were incubated on 6-well culture plates in an incubator for 24 hours, and were treated with the peptide of the present invention with different concentrations. After the incubation for 24 hours, the cells were dissolved, and the expression of tyrosinase, which is an essential enzyme involved in melanogenesis, was observed by western blot assay using a specific antibody. The test was conducted using an anti-tyrosinase antibody (Santa Cruz Biotechnology, USA).

When melanocytes B16F10 were treated with the peptide having the amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 2 and the expression aspect of the tyrosinase protein involved in melanogenesis was observed over time, it was verified that the expression of the protein was increased compared with a control in view of the 6-hour treatment and 24-hour treatment (FIGS. 4a and 4b).

US10093698B2. Peptide having efficacy for remedying hypopigmentation and inhibiting adipogenesis, and use of same (2018-10-09). CAREGEN CO., LTD. [KR]. Inventors: Yong Ji Chung [KR], Eun Mi Kim [KR], Eung Ji Lee [KR], Kyoung Mi Cho [KR].

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DOPA Reaction and Tyrosinase Immunostaining

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The DOPA reaction test was referred to the method of Gershoni-Baruch et
al. [10 (link)]. The whole brain was divided
sagittally and notches were coronally made at 1-mm intervals, and fixed immediately in a
solution which consisted of 10% formalin and 15% sucrose in 10 mM phosphate-buffered
saline (pH 7.4) at 4°C for 3 h. After fixation, each brain was embedded in
Tissue-Tek^® O.C.T. Compound (Sakura Finetek USA ; Torrance, CA, USA). The
cryostat sections with a 20-µm thickness adhered to a slide glass were
rinsed in PBS and incubated in 0.1% L-3,4-dihydroxyphenylalanine (DOPA) in 10 mM
phosphate-buffered saline (pH 7.4) at 37°C for 24 h, and then they were rinsed in water.
After the DOPA reaction, specimens were examined by light microscopy. Subsequently,
immunohistochemistry was carried out with anti-tyrosinase antibody (Santa Cruz) as a
primary antibody and RITC-conjugated donkey polyclonal antibody against goat IgG (1:100,
sc-2094: Santa Cruz) as a secondary antibody. Then, specimens were examined by
fluorescence microscopy.

Yamamuro Y, & Ogura S. (2018). Regional expression of tyrosinase in central catecholaminergic systems of colored mice. Experimental Animals, 68(1), 49-56.

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