Thirty minutes before the final detection, cultures were supplemented with propidium iodide at a concentration of 10 µM, in order to visualize dead cells. Cytotoxicity (PI staining of cells with damaged membranes) for hippocampal slices in all cultures was detected by fluorescence microscopy (AxionCam MRm, ZEISS, Germany). PI fluorescence reflects staining of necrotic or end-stage apoptotic cells. PI has a maximum excitation wavelength of 536 nm, and the emission of PI in the visual range is 620 nm (Kawalec et al. 2011 (link)).
Axioncam mrm
Manufactured by Zeiss
Sourced in Germany
The AxionCam MRm is a high-performance digital camera system designed for microscopy applications. It features a monochrome sensor with a resolution of 1.4 megapixels and a pixel size of 6.45 μm. The camera offers a high dynamic range and a frame rate of up to 45 frames per second, making it suitable for a wide range of microscopy techniques.
Automatically generated - may contain errors
Lab products found in correlation
6 protocols using axioncam mrm
1
Visualizing Cell Death in Hippocampal Slices
2
Histological Analysis of Liver Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Liver fragments were fixed in 10% formalin for 24 h, before being processed and embedded in paraffin. Five sections of 4–5 m from each group were cut and mounted on glass slides. The slices were stained with hematoxylin-eosin and examined by an inverted microscope (Observer Z1, Zeiss MicroImaging, GmbH) equipped with a camera and 4.7.4 image analysis software (AxionCam MRm Zeiss) at a magnification of 400x [24 (link)].
3
Fluorescent Imaging of Cell Morphology
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell morpholog y was assessed by direct fluorescence. After 24 and 72 h, the cells exposed to OCM-BioS-2P and OCM-Control were fixed in 4% paraformaldehyde (Merck, Darmstadt, Germany) for 10 min and permeabilized with Triton X-100 0.5% (Acros Organics, Geel, Belgium). Alexa Fluor 488 conjugated with phalloidin (Molecular Probes, Eugene, USA) 1:200, and 4',6-diamidino-2-phenylindole, dihydrochloride 300 (DAPI) (Molecular Probes) were used to stain the actin cytoskeleton and nuclei, respectively. Cell morphology was examined under epifluorescence using an Axio Imager microscope (Zeiss) attached to an AxionCam MRm (Zeiss) digital camera. The images were merged using the AxionVision 4.8 software (Zeiss) (n = 3).
4
Immunohistochemical Evaluation of PD-1, PD-L1, and sHLA-G
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sections of 5 µm thickness from cervical biopsies were submitted to IHC reactions using a Dako EnVision™ FLEX+ kit (Dako, Code: K8002, Santa Clara, CA, USA), following the manufacturer’s instructions. The primary antibodies were mouse anti-PD-1 antibody (ab52587; [NAT105] Abcam, Cambridge, UK), rabbit anti-PD-L1 monoclonal (ab213524; [EPR19759] Abcam), and mouse anti-soluble HLA-G (sHLA-G) isoforms 5 and 6 (5A6G7-Exbio, Vestec, Czech Republic). Protein expression was visualised and recorded using a microscope (Observer Z1, Zeiss MicroImaging, GmbH, Jena, Germany) equipped with a camera and image analysis software 2.1.0 (AxionCam MRm Zeiss). Brownish-brown staining revealed positive samples. Each slice was photographed in four fields. Three independent experts determined the pixel value acquired from the images using the Gimp 2.10.18 program (GNU Image Manipulation Program, UNIX platforms, www.gimp.org (accessed on 5 September 2023) and the mean values used in the analysis of protein expression in response to topical treatment with IMQ.
5
Histological Analysis of Liver Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Liver fragments were fixed in 10% formalin for 24 hours, before being processed and embedded in paraffin. Sections of 4–5μm were cut and mounted on glass slides. The sections were stained with hematoxylin-eosin (HE) and assessed with an inverted microscope (Observer Z1, Zeiss MicroImaging GmbH), equipped with a camera and 4.7.4 image analysis software (AxionCam MRm Zeiss), at a magnification of 400 x.
6
Histological Analysis of Liver Fibrosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Liver fragments were fixed in 10% formalin for 24 hours, before being processed and embedded in paraffin [30 (link)]. Five sections of 4-5 μm from each group were cut and mounted on glass slides. The slices were stained with hematoxylin-eosin and examined by an inverted microscope (Observer Z1, Zeiss MicroImaging, GmbH) equipped with a camera and 4.7.4 image analysis software (AxionCam MRm Zeiss) at a magnification of 400x. The fibrosis areas were quantified in five random fields on each slide using GIMP 2.6 imaging software [15 (link)].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!