All experimental animal protocols were performed following the Animal Care and Ethical Committee of Hebei Medical University (Shijiazhuang, China). C57BL/6 mice (C57) were obtained from the Charles River Labs. Atoh1-Cre: Restflox/flox conditional knockout mice (Rest cKO) were created by crossing Atoh1-Cre (Jackson Laboratories, Bar Harbor, ME, stock No. 011104) with Restflox/flox (kindly provided by Prof. Buckley and Prof. Gamper) mice. Rest cKO mice were in C57 background. BALB/c mice were purchased from Beijing Vital River Laboratory Animal Technology Co. Ltd (Beijing, China). PASS Sample Size Software was used to evaluate sample size. The experimental mice were grouped by a simple random sampling method and the relative assays were performed blindly. The mice were bred in-house under a 12:12 h light-dark cycle.
Atoh1 cre
Manufactured by Jackson ImmunoResearch
Atoh1-Cre is a Cre recombinase driver line that expresses Cre recombinase under the control of the Atoh1 (Hath1) promoter. Atoh1 is a transcription factor involved in the development of various cell types, including hair cells in the inner ear and cerebellar granule cells.
Automatically generated - may contain errors
Lab products found in correlation
Sox2 cre mice, by Jackson ImmunoResearch (1 mentions)
Pcp2 cre, by Jackson ImmunoResearch (1 mentions)
Nestin cre, by Jackson ImmunoResearch (1 mentions)
Neurod1 cre, by Jackson ImmunoResearch (1 mentions)
Balb c mice, by Charles River Laboratories (1 mentions)
C57bl 6j mice, by Jackson ImmunoResearch (1 mentions)
5 protocols using atoh1 cre
1
Conditional Knockout Mouse Model for Atoh1-Rest
2
Mouse Lines for Atoh1 Lineage Tracing
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The following mouse lines were used in this study: Atoh1lacZ/+ (catalog #005970, The Jackson Laboratory); Atoh1Cre/+ (Yang et al., 2010 (link)); Atoh1FlpO/+ (catalog #036541, The Jackson Laboratory); Atoh1flox/+(catalog #008681, The Jackson Laboratory); mRx-Cre (Klimova et al., 2013 (link)); Ai65 (catalog #021875, The Jackson Laboratory); and Ai14 (catalog #007914, The Jackson Laboratory). Ai65F mice were generated by crossing the Ai65 mice to Sox2-Cre mice (catalog #008454, The Jackson Laboratory). All mice were housed in a level 3, American Association for Laboratory Animal Science-certified facility on a 14 h light/dark cycle. Husbandry, housing, killing, and experimental guidelines were approved by the institutional animal care and use committee at Baylor College of Medicine.
3
Transgenic Mice Models for Cellular Analyses
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PTPMT1fl/+ mice were generated in our previous study (20 (link)). Nestin-Cre+ (21 (link)), Nestin-Cre+-Esr1 (25 (link)), PCP2-Cre+ (22 (link)), Atoh1-Cre+ (23 (link)), CAG-Cre+-Esr (24 (link)), and p53+/− (36 (link)) mice were purchased from the Jackson Laboratory. Mice of the same age, sex, and genotype were mixed and then randomly grouped for subsequent analyses (investigators were not blinded). All mice were kept under specific pathogen–free conditions in Division of Animal Resources at Emory University. All animal procedures complied with the National Institutes of Health Guide for the Care and Use of Laboratory Animals and were approved by the Institutional Animal Care and Use Committee.
4
Transgenic Mouse Models in Neuroscience
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Animals were maintained in an AAALAC-approved animal facility and all procedures were performed in accordance with IACUC guidelines (protocol number 17013). Pcp2/L7-Cre, Neurod1-Cre and Atoh1-Cre mice were obtained from The Jackson Laboratory (catalog numbers: 004146, 028364 and 011104). Memo1 mutant mice were generated using ES-cells from the European Conditional Mouse Mutagenesis (EUCOMM) repository as described previously (Van Otterloo et al., 2016 (link)). Mouse studies were allocated by genotype, and were not blinded.
5
Multispecies RNA-sequencing Protocols
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Animal procedures were approved by the Stanford University or the University of California Davis Animal Care and Use Committee and were carried out in accordance with NIH standards. We used 8-12 week old male C57BL/6J mice (Jackson Labs, #000664) for all mouse experiments, except for STARmap sequencing of rhombic lip-derived cells (Figs. 2C,S10). For this experiment, we used 8-12 week old male Atoh1-cre (Jackson Labs, #011104) × Ai14 (Jackson Labs, #007914) animals. Chicken snRNAseq was performed on adult (~20-week-old) male chickens that were the F1 progeny of a Line 6 × Line 7 cross from the Avian Disease and Oncology Laboratory (ADOL). Human samples were obtained from Donor Network West and were deemed exempt from IRB regulations by Stanford University. Both donor H1 and donor H2 were 65-year-old white males, and donor H3 was a 39-year-old black female. All died of cancer, with no brain involvement.
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