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2 protocols using p toluensulfonic acid monohydrate

1

Extraction and Analysis of Bioactive Compounds

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Sodium hydroxide, sodium chloride, aluminum chloride hexahydrate, p-toluensulfonic acid monohydrate (≥98.5), 2-chloro-4,4,5,5-tetramethyl-1,3,2-dioxaphospholane (95%), pyridine, chromium(iii)acetylacetonate, oleuropein, tyrosol, levulinic acid, 5-(hydroxymethyl)furfural, 3-(trimethylsilyl)propionic-2,2,3,3-d4 acid sodium salt, and pyrazine were purchased from Sigma-Aldrich (St. Louis, MO, USA). Hydrochloric acid 37% was provided from Panreac (Barcelona, Spain). Ethyl acetate was obtained from Scharlau (Barcelona, Spain) while maleic acid, CDCl3 (+0.03% vol. TMS), deuterium oxide, and cyclohexanol were purchased from Fluka™ (St. Gallen, Switzerland), VWR Chemicals (Solon, OH, USA), and Acros Organics (Geel, Belgium), respectively.
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2

Synthesis and Characterization of Functionalized Pluronic Hydrogels

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Pluronic F127® (Average Mw= 12600), acryloyl chloride, 4-dimethyl-aminopyridine (DMAP), 2,2-bis(hydroxymethyl)propionic acid (bis-MPA), acryloyl chloride, triethylamine (TEA), 1,3-dicyclohexylcarbodiimide (DCC), benzaldehyde dimethyl acetal, p-toluensulfonic acid monohydrate, 2-hydroxy-1-(4-(hydroxyethoxy) phenyl)-2methyl-1-propanone (Irgacure 2959, I2959), rhodamine B, CuSO 4 .5H 2 O, (L)-ascorbate and camptothecin were obtained from Sigma-Aldrich. Pluronic F127® was dried during 3 hours at 100 ⁰C under vacuum prior to use. All solvents were purchased from Sigma-Aldrich. 4-(dimethylamino) pyridinium 4-toluenesulfonate (DPTS), 6-azido-hexan-1ol, 2-(but-3-ynoyl)-2-methylpropane-1,3-diyl distearate (HCC-2C 18 ) [11] and tris(benzyltriazolylmethyl)amine [20] (TBTA) were synthesized according to previous reported procedures.
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