The dual Src/Abl inhibitor, bosutinib, was purchased from LC Laboratory (B-1788, Woburn, MA, USA). Doxorubicin (dox, D1515), etoposide (VP-16, E1383), and anti-β-Actin antibody (A2228) were purchased from Sigma (Sigma-Aldrich Corp, St. Louis, MO, USA). The antibodies against p-Src (Y416, 6943S), Src (2108S), p-c-Abl (Y245, 2868S), c-Abl (sc-56887), p-STAT3 (Y705, 4904S), STAT3 (4904S), p-ERK (T202/Y204, 9106L), ERK (4695S), p-S6 (Ser235/236) ribosomal protein (4858S), S6 ribosomal protein (2217S), Caspase 3 (9662S), PARP (9532S), and anti-Mouse (7076S) or anti-Rabbit (7074S) IgG were purchased from Cell Signaling Technology (Danvers, MA, USA).
Anti rabbit 7074s igg
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-Rabbit (7074S) IgG is a secondary antibody that specifically binds to rabbit primary antibodies. It is designed for use in various immunoassay techniques such as Western blotting, ELISA, and immunohistochemistry.
Automatically generated - may contain errors
Lab products found in correlation
Anti mouse 7076, by Cell Signaling Technology (4 mentions)
Anti β actin antibody a2228, by Merck Group (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Z vad fmk, by Adooq (1 mentions)
4 protocols using anti rabbit 7074s igg
1
Inhibition of Src/Abl Kinases in Cancer
2
Doxorubicin-induced Apoptosis Signaling
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TAK1 inhibitor NG25 (HY-15434) was purchased from MedChem Express (Monmouth Junction, NJ, USA) and was prepared according to the manufacturer’s recommendations. The antibodies against PARP (9532), Caspase 3 (9662), Caspase 7 (12827), IκBα (9242), phospho-p38 MAP kinase (Thr180/Tyr182) (9211), p38 MAP kinase (8690), anti-Mouse (7076S) and anti-Rabbit (7074S) IgG were purchased from Cell Signaling Technology (Danvers, MA, USA). Doxorubicin (Dox, D1515) and antibody against β-actin (A2228) were obtained from Sigma-Aldrich Corp (St. Louis, MO, USA).
3
Investigating Ponatinib and Doxorubicin Signaling Pathways
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Ponatinib was purchased from LC Laboratory (P-7022, Woburn, MA, USA). Purified mouse anti-basic FGF was purchased from BD biosciences (BDB610072, San Jose, CA, USA). Doxorubicin (Dox, D1515) and anti-β-Actin antibodies (A2228) were purchased from Sigma (Sigma-Aldrich Corp, St. Louis, MO, USA). The remaining antibodies—rabbit monoclonal p-AKT (Ser473) (4060S), rabbit polyclonal AKT (9272), rabbit monoclonal p-S6 (Ser235/236) ribosomal protein (4858S), rabbit monoclonal S6 ribosomal protein (2217S), rabbit monoclonal p-STAT3 (Y705) (9145L), rabbit monoclonal STAT3 (4904S), rabbit monoclonal PARP (9532S), rabbit polyclonal Caspase-3 (9662S), and anti-Mouse (7076S) or anti-Rabbit (7074S) IgG were purchased from Cell Signaling Technology (Danvers, MA, USA).
4
Evaluation of Bosutinib in Esophageal Cancer
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Cell lines. ECA109 and KYSE450 cell lines (purchased from the Cell Bank of the Chinese Academy of Sciences, Shanghai, China) were cultured in RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Zhejiang Tianhang Biological Technology Co., Zhejiang, China), penicillin (100 U/ml) and streptomycin (100 mg/ml) (Gibco; Thermo Fisher Scientific, Inc.). All cells were maintained in humidified incubator at a constant temperature of 37 °C and 5% CO 2 .
Antibodies and reagents. Bosutinib was purchased from LC Laboratory (B-1788, Woburn, MA, USA). Doxorubicin (dox, D1515) and anti-β-Actin antibody (A2228) were purchased from Sigma (Sigma-Aldrich, St. Louis, USA). ZVAD-FMK was from Adooq Bioscience (CA, USA). The antibodies against p-Src (Y416, 6943S), Src (2108S), p-c-Abl (Y245, 2868S), c-Abl (sc-56887), p-STAT3 (Y705, 4904S), STAT3 (4904S), p-S6 (Ser235/236) ribosomal protein (4858S), S6 ribosomal protein (2217S), Caspase 3 (9662S), PARP (9532S), and anti-mouse (7076S) or antirabbit (7074S) IgG were purchased from Cell Signaling Technology (Danvers, MA, USA).
Antibodies and reagents. Bosutinib was purchased from LC Laboratory (B-1788, Woburn, MA, USA). Doxorubicin (dox, D1515) and anti-β-Actin antibody (A2228) were purchased from Sigma (Sigma-Aldrich, St. Louis, USA). ZVAD-FMK was from Adooq Bioscience (CA, USA). The antibodies against p-Src (Y416, 6943S), Src (2108S), p-c-Abl (Y245, 2868S), c-Abl (sc-56887), p-STAT3 (Y705, 4904S), STAT3 (4904S), p-S6 (Ser235/236) ribosomal protein (4858S), S6 ribosomal protein (2217S), Caspase 3 (9662S), PARP (9532S), and anti-mouse (7076S) or antirabbit (7074S) IgG were purchased from Cell Signaling Technology (Danvers, MA, USA).
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