C57bl 6 b6 mice

Manufactured by Jackson ImmunoResearch

Sourced in United States, Montenegro, Japan

C57BL/6 (B6) mice are a widely used inbred mouse strain. They are a common laboratory animal model for research purposes.

Automatically generated - may contain errors

Lab products found in correlation

131 protocols using c57bl 6 b6 mice

Murine Model of Viral Infection Study

Check if the same lab product or an alternative is used in the 5 most similar protocols

Specific pathogen-free female C57BL/6 (B6) mice (The Jackson Laboratory, JAX:000664) at 5 to 6 weeks of age were purchased from Japan SLC, Inc., and maintained under barrier conditions in a biosafety level 3 (BSL-3) biohazard animal room at the Medical Research Center of Yonsei University College of Medicine and B6 mice at 6 to 8 weeks of age were used for experiments. P25 (C57BL/6-Tg(H-2Kb-Tcra,Tcrb)P25Ktk/j)(The Jackson Laboratory, JAX:011005) mice were purchased from Jackson Laboratory (Bar Harbor, ME)^{45 (link)}. Ifnar1^−/− mice (B6.129S2-Ifnar1tm1Agt/Mmjax) (The Jackson Laboratory, JAX:32045) were kindly gifted from Dr. Heung Kyu Lee (KAIST, Daejun, Republic of Korea). CD45.1 mice (B6.SJL-Ptprca Pep3b/BoyJ) (The Jackson Laboratory, JAX:002014) were obtained from laboratory of Young-Chul Sung (POSTECH, Republic of Korea). For experiments, each female mice at 6 to 8 weeks of age were used and background strain of all mice used is C57BL/6 (B6) mice (The Jackson Laboratory, JAX:000664). Animal maintenance and procedures were performed with approval of the IACUC of Yonsei University College of Medicine (permit number: 2016-0305). All animal studies were performed in accordance with Korean Food and Drug Administration (KFDA) guidelines. The mice were housed at temperature (23 ± 3°C), humidity range 40–60, and 12 h light-dark cycle (7 am on and 7 pm off).

Kang T.G., Kwon K.W., Kim K., Lee I., Kim M.J., Ha S.J, & Shin S.J. (2022). Viral coinfection promotes tuberculosis immunopathogenesis by type I IFN signaling-dependent impediment of Th1 cell pulmonary influx. Nature Communications, 13, 3155.

+ Open protocol

+ Expand

Transgenic Mouse Models for Immunological Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols

C57BL/6 mice (B6) from Jackson Laboratory (Figs. 2, 3, 5D; Bar Harbor, ME) or Taconic Farms (Figs. 1, 4, 5A-C, 5E, 6; Germantown, NY or Cambridge City, IN) were used. OT-II mice were purchased from Jackson Laboratory. Il22^−/− mice (24 (link)) were bred at Taconic Farms (Cambridge City, Indiana). Mice with a targeted deletion for all six exons of RegIIIγ were generated as described below, and backcrossed to B6 for ten generations. Mice were housed at Louisiana State University Health Sciences Center and the University of Pittsburgh under specific pathogen-free conditions, and were handled in accordance to NIH federal guidelines.

McAleer J.P., Nguyen N.L., Chen K., Kumar P., Ricks D.M., Binnie M., Armentrout R.A., Pociask D.A., Hein A., Yu A., Vikram A., Bibby K., Umesaki Y., Rivera A., Sheppard D., Ouyang W., Hooper L.V, & Kolls J.K. (2016). Pulmonary Th17 anti-fungal immunity is regulated by the gut microbiome. Journal of immunology (Baltimore, Md. : 1950), 197(1), 97-107.

+ Open protocol

+ Expand

Qa-1 Knockout Mice Study

Check if the same lab product or an alternative is used in the 5 most similar protocols

C57BL/6 mice (B6) were purchased from the Jackson Laboratory. Qa-1^b−/− mice were gift from Dr. Harvey Cantor (Harvard Medical School) (35 (link)). All the mice were maintained in the University of Utah specific pathogen-free animal facility and used according to the protocol approved by the Institutional Animal Care and Use Committee of University of Utah.

Chen L., Reyes-Vargas E., Dai H., Escobar H., Rudd B., Fairbanks J., Ho A., Cusick M.F., Kumánovics A., Delgado J., He X, & Jensen P.E. (2014). Expression of the mouse MHC class Ib H2-T11 gene product, a paralog of H2-T23 (Qa-1) with shared peptide-binding specificity. Journal of immunology (Baltimore, Md. : 1950), 193(3), 1427-1439.

+ Open protocol

+ Expand

Transgenic Mouse Strains for Immunology

Cited 4 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Inbred B6 and CD45.1⁺ congenic C57BL/6 (B6) mice were originally obtained from The Jackson Laboratory (Bar Harbor, Maine, USA). TCRβ-transgenic EF4.1 mice (21 (link)), Ifngr1^−/− mice (25 (link)), Ifnar1^−/− mice (26 (link)), Tnfr1^−/− mice (27 (link)), Tnfrsf4^−/− mice (28 (link)), Il21r^−/− mice (29 (link)), Il12a^−/− mice (30 (link)) and Nur77-green fluorescent protein GFP transgenic mice (31 (link)) were all on the B6 genetic background. Mice with dendritic cell (DC)-specific deletion of MHC class II were obtained by crossing mice with a conditional H2-Ab1 allele (H2-Ab1^fl) (32 (link)) with mice expressing Cre under the Cd11c promoter (33 (link)). In the latter strain, Cre-mediated recombination is observed in nearly all CD11c⁺ DCs, but not in CD11c⁻ monocytes/macrophages, whereas only partial recombination is observed in CD11c^low monocytes, attributed to their differentiation into DCs (33 (link)). All animal experiments were approved by the ethical committee of the NIMR, and conducted according to local guidelines and UK Home Office regulations under the Animals Scientific Procedures Act 1986 (ASPA).

Thorborn G., Ploquin M.J., Eksmond U., Pike R., Bayer W., Dittmer U., Hasenkrug K.J., Pepper M, & Kassiotis G. (2014). Clonotypic Composition of the CD4+ T Cell Response to a Vectored Retroviral Antigen Is Determined by Its Speed. The Journal of Immunology Author Choice, 193(4), 1567-1577.

+ Open protocol

+ Expand

Conditional Bcor Allele Generation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The conditional Bcor allele (Bcor^fl), which contains LoxP sites flanking Bcor exons 9 and 10, was generated by homologous recombination (Wamstad et al, manuscript in preparation). Cre-mediated deletion results in a premature stop codon and a null allele. Bcor^fl/+ mice were backcrossed with C57BL/6NCr mice (NCI Frederick) for >6 (Fig. 1) or >10 generations (Fig. 3–4). B6.Cg-Tg(Lck-cre)3779Nik/J (The Jackson Laboratory) males were bred to Bcor^fl/+ females to generate wild-type (WT;Bcor^+/Y or Bcor^fl/Y Lck-Cre⁻) and T cell BCOR-deficient (Bcor^fl/Y Lck-Cre⁺) males. C57BL/6 (B6 mice) (The Jackson Laboratory) used in Fig. 2 were housed in specific pathogen-free conditions while other mice were housed in a conventional facility at the University of Minnesota. All experimental protocols were performed in accordance with guidelines of the University of Minnesota Institutional Animal Care and Use Committee and National Institutes of Health.

Yang J.A., Tubo N.J., Gearhart M.D., Bardwell V.J, & Jenkins M.K. (2015). BCL6 interacting corepressor contributes to germinal center T follicular helper cell formation and B cell helper function. Journal of immunology (Baltimore, Md. : 1950), 194(12), 5604-5608.

+ Open protocol

+ Expand

Sourcing Transgenic Mouse Strains

Check if the same lab product or an alternative is used in the 5 most similar protocols

C57BL/6 (B6) mice were purchased from the Jackson Laboratory. B6-Kb⁰Db⁰;B7.2 transgenic (B7tg) mice were obtained with permission from Drs. Alexander Sette (La Jolla Institute for Allergy and Immunology, La Jolla, CA) and Francois Lemonnier (Institut Pasteur, Paris, France). PD-1^−/− mice were obtained with permission from Dr. Tasuku Honjo (Kyoto University, Kyoto, Japan). All animals were bred and maintained in specific pathogen-free conditions in accordance with the Vanderbilt Institutional Animal Care and Use Committee. 6–12 week old age- and gender-matched animals were used in all experiments.

Erickson J.J., Rogers M.C., Tollefson S.J., Boyd K.L, & Williams J.V. (2016). Multiple inhibitory pathways contribute to lung CD8+ T cell impairment and protect against immunopathology during acute viral respiratory infection. Journal of immunology (Baltimore, Md. : 1950), 197(1), 233-243.

+ Open protocol

+ Expand

Breeding C57BL/6 Mice for Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

C57BL/6 (B6) mice were purchased from The Jackson Laboratory in Bar Harbor, ME, USA, and bred in the animal care facility at the C.S. Mott Center for Human Growth and Development at Wayne State University, Detroit, MI, USA. All mice were kept under a circadian cycle (light:dark = 12:12h). Females, 8–12 weeks old, were mated with males of the same phenotype. Female mice were checked daily between 8:00 a.m. and 9:00 a.m. for the appearance of a vaginal plug, which indicated 0.5 days post coitum (dpc). Females were then housed separately from the males, their weight was monitored, and a gain of two or more grams by 12.5 dpc confirmed pregnancy. All procedures were approved by the Institutional Animal Care and Use Committee (IACUC) at Wayne State University (Protocol Nos. A-09-08-12 and A-07-03-15).

Gomez-Lopez N., Romero R., Plazyo O., Panaitescu B., Furcron A.E., Miller D., Roumayah T., Flom E, & Hassan S.S. (2016). Intra-amniotic Administration of HMGB1 Induces Spontaneous Preterm Labor and Birth. American journal of reproductive immunology (New York, N.Y. : 1989), 75(1), 3-7.

+ Open protocol

+ Expand

Adoptive T-cell Therapy for Melanoma

Check if the same lab product or an alternative is used in the 5 most similar protocols

On day 0 C57BL/6 (B6) mice (female, eight weeks, Jackson Laboratories) were injected (s.c.) with 10⁶ B16-SIY melanoma cells into the right flank. Five days later all tumor-bearing mice received 5×10⁶ activated 2C cells (i.v.) and 30.000 U/mouse IL-2 (i.p.). Mice were treated on days 6, 7 and 8 with 10⁷ aAPC or aAPC^CD47+ (i.v.) and 30.000 U/mouse IL-2 (i.p.). Tumor growth was monitored every other day utilizing digital caliper. aAPC were generated with ^SIY-K^b-Ig, anti-mouse-CD28 and rm-CD47-Ig (aAPC^CD47+). B6, NOD/SCID and 2C TCR Rag^−/− transgenic mice were maintained in the Johns Hopkins animal facilities and procedures involving animals and their care were in conformity with institutional guidelines that comply with national and international laws and policies. Statistical analysis was performed in GrapPad Prism5.

Bruns H., Bessell C., Varela J.C., Haupt C., Fang J., Pasemann S., Mackensen A., Oelke M., Schneck J.P, & Schütz C. (2015). CD47 enhances in vivo functionality of artificial antigen-presenting cells. Clinical cancer research : an official journal of the American Association for Cancer Research, 21(9), 2075-2083.

+ Open protocol

+ Expand

Ophthalmic Research Protocol in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

This research protocol was approved by the Baylor College of Medicine Center for Comparative Medicine, and it conformed to the standards of the Association for Research in Vision and Ophthalmology Statement for the Use of Animals in Ophthalmic and Vision Research. Female C57BL/6 (B6) mice were purchased from Jackson Laboratories (Bar Harbor, ME) and were used at 6-8 weeks of age.

de Paiva C.S., Jones D.B., Stern M.E., Bian F., Moore Q.L., Corbiere S., Streckfus C.F., Hutchinson D.S., Ajami N.J., Petrosino J.F, & Pflugfelder S.C. (2016). Altered Mucosal Microbiome Diversity and Disease Severity in Sjögren Syndrome. Scientific Reports, 6, 23561.

+ Open protocol

+ Expand

Animal Care and Experimental Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice were cared for at the UConn Health animal facility and fed ad libitum. All experimental protocols conform to federal regulatory standards, and were approved by the UConn Health Institutional Animal Care Committee (IACUC), Farmington, CT. C57BL/6 (B6) mice were purchased from Jackson Laboratory, Bar Harbor ME. TEα and CD11c-Eα^{hi and low} transgenic mice were bred in-house. Experiments described in Tables S1 and S2, Figures 1, 2, 3, 4, and 5 and S1 and S2 utilized both male and female adult mice that ranged between 6 weeks to 6 months of age. Adult 6-week to 3-month-old females were used exclusively for the tumor immunotherapy studies described in Figures 6 and 7 and S3 and S4.

Del Valle C.M., Maxwell J.R., Xu M.M., Menoret A., Mittal P., Tsurutani N., Adler A.J, & Vella A.T. (2019). Costimulation Induces CD4 T Cell Antitumor Immunity via an Innate-like Mechanism. Cell reports, 27(5), 1434-1445.e3.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!