A rotarod apparatus (Ugo Basile) was used to assess the motor coordination skills, as previously done.44 The rotarod apparatus consists of five 3 cm diameter cylinders, which are suitably machined to provide grip. Six 25 cm diameter dividers make for five lanes, each 5.7 cm wide, enabling five mice to be on the rotor simultaneously. Mice were placed on the rotating rod suspended horizontally at a height of 16 cm from the floor. Four trials per day with an ITI of 15 min and a cutoff of 5 min at 4 rpm speed were performed for each animal and the mean latency to fall was calculated.
Rotarod apparatus
Manufactured by Ugo Basile
Sourced in Italy
The Rotarod apparatus is a device used to assess motor coordination and balance in laboratory animals. It consists of a rotating rod or cylinder that the animal must walk on to avoid falling. The speed of rotation can be adjusted to increase the difficulty of the task.
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Lab products found in correlation
116 protocols using rotarod apparatus
1
Rotarod Assessment of Motor Coordination
2
Rotarod Test for Motor Coordination
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The Rotarod test was carried out every 5 days from PND 25–28. As was previously done, a rotarod apparatus (Ugo Basile, Varese, Italy) was used to assess the motor coordination skills [14 (link)].
The rotarod apparatus consisted of five 3 cm diameter cylinders, which were suitably machined to provide grip. Six 25 cm diameter dividers made five lanes, each 5.7 cm wide, enabling five mice to be on the rotor simultaneously. The mice were placed on the rotating rod suspended horizontally at a height of 16 cm from the floor. Four trials per day with an inter-trial interval (ITI) of 15 min and a cut-off of 5 min at 4 rpm speed were performed for each animal and the mean latency to fall was calculated.
The rotarod apparatus consisted of five 3 cm diameter cylinders, which were suitably machined to provide grip. Six 25 cm diameter dividers made five lanes, each 5.7 cm wide, enabling five mice to be on the rotor simultaneously. The mice were placed on the rotating rod suspended horizontally at a height of 16 cm from the floor. Four trials per day with an inter-trial interval (ITI) of 15 min and a cut-off of 5 min at 4 rpm speed were performed for each animal and the mean latency to fall was calculated.
3
Rotarod Evaluation of Motor Coordination
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The rotarod apparatus (Ugo Basile, Comerio, Italy) was used to measure motor coordination, balance, and motor learning ability (Miana-Mena et al., 2005 (link); Crawley, 2008 (link)). A good performance requires a high degree of sensorimotor coordination. It consists of a computer-controlled motor-driven rotating spindle and five lanes for five mice. Falls of the mice are detected automatically by pressure on a plastic plate at the bottom. After training for three times at a constant speed of 15 rpm and for the duration of 180 s, mice were tested every 3 days beginning on d80 and the time for which an animal can remain on the rotating rod is measured. For animals which could not remain 180 s on the rotarod, a second and third trial with a break of 10 min was applied. Means of all trials of each animal were included in the statistical analysis. The time of 180 s is chosen as an arbitrary cut-off time because the majority of significant differences in motor coordination are detected in this time frame.
4
Rotarod Assay for Mouse Locomotor Function
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On the first 2 days, mice were placed on a rotarod apparatus (Ugo Basile, Italy) that accelerated 5 to 20 rpm for 5 min and were trained to maintain their balancing walking. On the third day, rod accelerated 5 to 40 rpm, and mice were tested with a maximum time of 300 s. The latencies of animals to fall off were recorded. All behavioral testing and analysis were done by an observer blinded to the experimental groups.
5
Evaluating Motor Deficits in Tau Mouse Model
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Motor deficits in wild-type and THY-Tau22 mice were evaluated by testing them on a rotarod apparatus (Ugo Basile), as described previously. 25 Briefly, animals were first submitted to training sessions (three trials per day during 3 consecutive days before injection) during which they were placed on the rod rotating with a progressive acceleration from 4 to 40 rpm. Animals were individually separated the day before the test and evaluated using the same experimental setting throughout 300 seconds. The latency to fall off the rotarod was recorded. Animals staying >300 seconds were removed from the rotarod, and their latency fall recorded as 300 seconds. Mice were tested 1, 2, and 3 months after injection.
6
Rotarod Assessment of Motor Coordination
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Motor coordination of mice was assessed using a rotarod apparatus (UGO BASILE, Comerio, VA, Italy) at an accelerated speed (4~44 rpm) over a period of 6 min. The animals were pre-trained for one trial at an accelerated speed (4~44 rpm) for 5 min 2 days before the real test to allow them to become acquainted with the rotarod apparatus. Heterozygous (n = 15) and homozygous (n = 8) Hdh(CAG)150 mice and their littermates (n = 15) were then tested every 3 months from 7 to 19 months of age. The rotarod test of R6/2 mice (n = 15) and the WT littermates (n = 15) were performed every week from 6~12 weeks of age. R6/2 mice treated with (n = 18) and without NAC (n = 18) and the WT littermates (n = 18) treated with saline were tested every week from 7 to 12 weeks of age. Each mouse was tested for a maximum of 6 min per trial for 3 trials with an interval of 30 min in a day and mean of the 3 trials was used for comparison between groups. Latency to falling was automatically recorded.
7
Evaluating Motor Coordination in Mice
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Motor coordination was evaluated in a Rotarod apparatus (Ugo Basile) with increasing acceleration. The apparatus consisted of a horizontal motor-driven rotating rod in which the animals were placed perpendicular to the long axis of the rod, with the head placed against the direction of rotation so that the mouse must move ahead in order to avoid falling. The trial was stopped when the animal fell down or after a maximum of 5 min. The time spent in the rotating rod was recorded for each animal and trial. Animals received a pretraining session to familiarize them with the procedure prior to evaluation. Thereafter, a total of six consecutive trials were conducted done for each animal. The data are presented as the average time spent before falling from the apparatus.
8
Rotarod Test for Motor Function
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Rotarod test was performed based on the previously validated methods [32 (link)] with minor modifications. All animals were subjected to the rotarod test by a blinded investigator. On the testing day, animals were first pre-trained on the rotarod apparatus (UgoBasile) 1 h before the main trial. Mice were located on a rotating rod accelerating from 5 to 20 rpm for a 300 s time period. The results were recorded as the time in seconds at which the mice fell off from the rung of the rod, and the mice that completed the task over 300 s scored as 300.
9
Rotarod Performance Evaluation
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The motor coordination was assessed using a Rotarod apparatus (UGO BASILE, Comerio, Italy) at a constant speed (12 rpm for 2 min). All mice were tested three times per week. Each test session comprised three trials for each mouse. The latency to fall from the rotating rod, up to a maximum of 2 min, was recorded for each trial. Weekly maximum performance for each mouse was used for statistical analysis.
10
Assessing Coordination Using Rotarod
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A rotarod apparatus (Ugo Basile) was used to assess coordination skills. After two acclimation sessions, the mice underwent three trial sessions at least 20 min apart, using a standard acceleration protocol.
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