Infinite m1000 plate reader

Manufactured by Tecan

Sourced in Switzerland, United States, Austria, Germany

The Infinite M1000 is a microplate reader designed for a wide range of absorbance, fluorescence, and luminescence-based applications. It features a flexible monochromator-based optical system, allowing users to select arbitrary wavelengths for measurements. The Infinite M1000 supports 6- to 384-well microplates and provides accurate and sensitive detection across multiple detection modes.

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Lab products found in correlation

Alamarblue reagent, by Thermo Fisher Scientific (5 mentions) Celltiter glo, by Promega (3 mentions) Caspase glo 3 7 assay, by Promega (3 mentions) Prism 5, by GraphPad (3 mentions) Spectramax 340pc, by Tecan (2 mentions) Nunc maxisorp, by Thermo Fisher Scientific (2 mentions) Celltiter glo luminescent cell viability assay kit, by Promega (2 mentions) K 3200, by Echelon Biosciences (2 mentions) Rnasin, by Promega (2 mentions) Tween 20, by Merck Group (2 mentions) Thioflavint dye, by Merck Group (2 mentions) Nunc 96 well black optical bottom plates, by Thermo Fisher Scientific (2 mentions) Pierce 660 nm protein assay, by Thermo Fisher Scientific (2 mentions) Rabbit reticulocyte lysate system, by Promega (2 mentions) Camp glo sensor 20f, by Promega (2 mentions) Luciferase assays substrate, by Promega (2 mentions) Caspase glo 3 7 assay kit, by Promega (2 mentions) Celltiter glo reagent, by Promega (1 mentions) Kaleidagraph 4, by Synergy Software (1 mentions) 75 cm2 culture flask, by Corning (1 mentions)

Spelling variants of this product

Infinite M1000 (648 citations) M1000 (189 citations) Infinite M1000 Pro plate reader (183 citations) M1000 plate reader (100 citations) Infinite plate reader (36 citations) Infinite M1000 reader (23 citations) Infinite M1000 Pro Multimode Plate Reader (6 citations) Infinite M1000 plate (4 citations) Infinite M1000 multimode plate reader (4 citations) Reader M1000 (2 citations)

164 protocols using infinite m1000 plate reader

Mitochondrial Function Assays

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Mitochondrial membrane potential was determined using tetramethylrhodamine (TMRM), a potentially sensitive dye. Cells were transfected with si-NT or si-m/hVDAC1-B and, 48 h post-transfection, were seeded in 96 white plates, incubated with TMRM (800 nM, 20 min, 37 °C, 5% CO₂). The cells were then washed twice with PBS and incubated with media (contains 1% FBS) without phenol red. TMRM fluorescence was measured with an Infinite M1000 plate reader (Tecan, Männedorf, Switzerland).
Cellular ATP levels were estimated using a luciferase-based assay (CellTiter-Glo, Promega) according to the manufacturer’s protocol, and luminescence was recorded using an Infinite M1000 plate reader (Tecan, Männedorf, Switzerland).

Alhozeel B., Pandey S.K., Shteinfer-Kuzmine A., Santhanam M, & Shoshan-Barmatz V. (2024). Silencing the Mitochondrial Gatekeeper VDAC1 as a Potential Treatment for Bladder Cancer. Cells, 13(7), 627.

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Cell Viability Measurement Assays

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Viability was assessed using AlamarBlue® reagent (Invitrogen) or Cell-Titer-Glo® reagent (Promega) assay according to manufacturer’s protocol. Fluorescence was detected at 560 nm on InfiniteM1000 plate reader and luminescence on InfiniteM1000 plate reader (Tecan) or a Spectramax 340PC.

Pu S.Y., Wouters R., Schor S., Rozenski J., Bentov R.B., Prugar L.I., O’Brien C.M., Brannan J.M., Dye J.M., Herdewijn P., De Jonghe S, & Einav S. (2018). Optimization of isothiazolo[4,3-b]pyridine-based inhibitors of cyclin G associated kinase (GAK) with broad-spectrum antiviral activity. Journal of medicinal chemistry, 61(14), 6178-6192.

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Cell Viability Assay Protocol

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Viability was assessed using alamarBlue reagent (Invitrogen) or CellTiter-Glo reagent (Promega) assay according to manufacturer’s protocol. Fluorescence was detected at 560 nm on an InfiniteM1000 plate reader and luminescence on an InfiniteM1000 plate reader (Tecan) or a SpectraMax 340PC.

Pu S., Schor S., Karim M., Saul S., Robinson M., Kumar S., Prugar L.I., Dorosky D.E., Brannan J., Dye J.M, & Einav S. (2020). BIKE regulates dengue virus infection and is a cellular target for broad-spectrum antivirals. Antiviral research, 184, 104966.

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Fluorescence Anisotropy Assays for Protein-DNA Binding

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Fluorescence anisotropy assays were performed in assay buffer (50 mM Tris/HCl pH 8.0, 150 mM NaCl, 1 mM TCEP, 0.1 mg/ml BSA) using a TECAN infinite M1000 plate reader (Tecan Group Ltd., Männedorf, Switzerland) and 480 nm excitation/520 nm emission wavelengths. His₆-tagged AvrBs3 and variants were purified under reducing conditions and used at concentrations sufficient to saturate dsDNA E1U20 fragment [6 (link)] binding at the highest concentration. A protein dilution series (factor 0.6) was titrated with 0.5 nM fluoresceine-labeled DNA. DNA and protein mixtures were incubated in a 96-well plate for 10 min at RT before readout. Values are the means of three independent measurements wherein each measured point was set up in duplicate. Binding data were analyzed using Kaleidagraph 4.0 (Synergy Software, Reading, USA) and corrected with a linear equation for unspecific binding and using a simple model assuming one binding site per DNA fragment and a 1:1 stoichiometry. For comparison, fluorescence polarization units were normalized; 100% represents saturated binding.

Schreiber T., Sorgatz A., List F., Blüher D., Thieme S., Wilmanns M, & Bonas U. (2015). Refined Requirements for Protein Regions Important for Activity of the TALE AvrBs3. PLoS ONE, 10(3), e0120214.

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Quantifying Active MMP-2 Levels

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MMP-2 activity was measured using the Amersham Biotrak Activity Assay System (GE Healthcare UK Ltd, Buckinghamshire, UK). Cells were plated, allowed to attach in complete growth media, washed with PBS and changed to serum-free media. As well as a serum-free media only control, one group received IGF-1 (50 ng/ml) and the third received IGF-1 (50 ng/ml) and Nutlin-3 (1 μM). Cells were incubated with their respective medias for 12 h, after which time the conditioned media was collected and cells discarded. Levels of active MMP-2 in the collected conditioned media were assayed by following the MMP-2 Biotrack Activity Assay manufacturer’s protocol. Briefly; conditioned media was added to an anti-MMP-2-coated 96-well microplate to capture endogenous MMP-2. Following washing steps, MMP-2 levels are detected by way of a detection reagent and optical density 405-nm reading using a TECAN infinite M1000 plate reader (Tecan Group Ltd), and signal conversion using a standard curve of known MMP-2 concentration. Quantification of the total and endogenously active MMP-2 was performed by adding p-aminophenylmercuric acetate solution to obtain a ‘Total MMP-2’ for that sample.

Worrall C., Suleymanova N., Crudden C., Trocoli Drakensjö I., Candrea E., Nedelcu D., Takahashi S.I., Girnita L, & Girnita A. (2017). Unbalancing p53/Mdm2/IGF-1R axis by Mdm2 activation restrains the IGF-1-dependent invasive phenotype of skin melanoma. Oncogene, 36(23), 3274-3286.

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Evaluating Thyroid Hormone Activity with Luciferase Assay

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Thyroid hormone activity was measured by the assay described previously with slight modifications.^{14 (link)} GH3.TRE-Luc cells were seeded at 80% confluency in 75 cm² culture flasks (Corning, Schiphol-Rijk, Netherlands) in regular growth medium. Cells were collected and seeded into 24-well plates for an additional period of 24 h, and incubated for 24 h in the presence or absence of T₃, with the indicated test chemical in DMSO. The DMSO concentration in 500 μL of exposure medium was always the same for all exposures within an experiment and always kept ≤ 0.5% (v/v) to avoid cytotoxicity. Cell viability in each well was determined by measuring total protein concentration using the bicinchoninic acid (BCA) assay following the manufacturer's protocol. Luciferase activity was measured on lysed cells on an Infinite M1000 plate reader (Tecan Group AG, Zürich, Switzerland).

Kitamura S., Hvorecny K.L., Niu J., Hammock B.D., Madden D.R, & Morisseau C. (2016). Rational design of potent and selective inhibitors of an epoxide hydrolase virulence factor from Pseudomonas aeruginosa. Journal of medicinal chemistry, 59(10), 4790-4799.

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Glutathione Measurement in Cells

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The level of glutathione was measured using an EarlyTox Glutathione Assay Kit (Molecular Devices, Sunnyvale, CA, USA). ~2 × 10⁴ cells were seeded per well (100 μl culture medium) of a 96-well black clear F-bottom plate (Greiner bio-one GmbH, Frickenhausen, Germany) and incubated at 37°C and at 21% and 5% O₂, respectively, overnight. Cells treated with 2 μM staurosporine (Cell Signaling Technology, Danvers, MA, USA) which inhibits protein C kinase and other kinases leading to cell death, and a decrease in GSH served as a positive control for a decrease in GSH level. The assay was performed 1 h and 18 h after X-ray irradiation or 6 h and 24 h after Aβ peptide addition by adding 40 μM monochlorbimane (MCB) directly to the cell culture media. Cells were incubated at 37°C, and fluorescence of the MCB-S-glutathione conjugate was measured using an Infinite M1000 plate reader (Tecan Group Ltd., Männedorf, Switzerland) with the 394 nm excitation filter and 490 nm emission filter. The intensity of the fluorescence signal is directly proportional to the level of GSH in the cells.

Džinić T, & Dencher N.A. (2018). Oxygen Concentration and Oxidative Stress Modulate the Influence of Alzheimer's Disease Aβ 1–42 Peptide on Human Cells. Oxidative Medicine and Cellular Longevity, 2018, 7567959.

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Cinacalcet Modulates Calcium Signaling

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T84 cells were plated in 96-well, black-walled microplates (Corning Life Sciences) and used at 72 hours after plating. Confluent cells were loaded with calcium indicator Fluo-4 NW (Invitrogen, Thermo Fisher Scientific) per manufacturer’s instructions. Fluo-4 fluorescence was measured in each well continuously with a Tecan Infinite M1000 plate reader (Tecan Group) at excitation/emission wavelengths of 495 nm/516 nm after manual addition of 30 μM cinacalcet (or 1% DMSO vehicle control). In some studies cells were pretreated with PLC inhibitor U73122 (10 μM) for 5 minutes prior to addition of cinacalcet. For cAMP assay, T84 cells were grown in clear 24-well plates and pretreated for 20 minutes with 30 μM cinacalcet with or without 500 μM IBMX or vehicle control (0.2% DMSO). Then cells were treated with 10 μM forskolin (for 5 minutes) and lysed by repeated freeze/thaw and centrifuged to remove cell debris. The supernatant was assayed for cAMP using the cAMP Parameter immunoassay kit according to the manufacturer’s instructions (R&D Systems, Bio-Techne).

Oak A.A., Chhetri P.D., Rivera A.A., Verkman A.S, & Cil O. (2021). Repurposing calcium-sensing receptor agonist cinacalcet for treatment of CFTR-mediated secretory diarrheas. JCI Insight, 6(4), e146823.

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Collagen-induced Platelet Adhesion Assay

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Washed platelets (1 × 10⁸ platelet/mL) were added to 96-well assay plates (Nunc MaxiSorpTM Thermo Fisher Scientific, Waltham, MA, USA) previously coated overnight with 5 μg/mL Horm® collagen (Takeda Austria GmbH, Linz, Austria), and incubated for 1 h at 37 °C. Unbound platelets were separated by washing the plates with PBS three times. Then, p-nitrophenyl phosphate-based solution was added to plates for 40 min with agitation. Reactions were stopped with 50 μL of 3 M NaOH and the absorbance (405 nm) was measured in a Tecan Infinite^® M1000 plate reader (Tecan Group Ltd., Männedor, Switzerland). Each assay was run three times and absorbance values (arbitrary units) were normalized with the negative control (BSA condition).

Barrachina M.N., Izquierdo I., Hermida-Nogueira L., Morán L.A., Pérez A., Arroyo A.B., García-Barberá N., González-Conejero R., Troitiño S., Eble J.A., Rivera J., Martínez C., Loza M.I., Domínguez E, & García Á. (2021). The PI3Kδ Inhibitor Idelalisib Diminishes Platelet Function and Shows Antithrombotic Potential. International Journal of Molecular Sciences, 22(7), 3304.

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Caspase-3/7 Activity Quantification

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Caspase activity was measured by the luminescent Caspase-Glo® 3/7 Assay (Promega). This homogeneous, luminescent assay provides a luminogenic caspase 3/7 substrate, which contains the tetrapeptide sequence DEVD, in a reagent optimized for caspase activity, luciferase activity, and cell lysis. The protocol was performed according to the manufacturer’s guidelines. In short, for in vitro applications, equal amounts of Caspase-Glo® 3/7 reagent and PBS were added to the cultured neurons upon removal of the medium. Buffer and cells were mixed and incubated for 30–45 min. For whole cortex measurements, cortices were isolated and lysed in 10 µl PBS per mg of cortex. Equal volumes of cell lysate and Caspase-Glo® 3/7 reagent were mixed and incubated for 90 min. Luminescence was measured with a Tecan infinite M1000 plate reader (Tecan Group) and normalized to controls for statistical analysis.

Schroer J., Warm D., De Rosa F., Luhmann H.J, & Sinning A. (2023). Activity-dependent regulation of the BAX/BCL-2 pathway protects cortical neurons from apoptotic death during early development. Cellular and Molecular Life Sciences, 80(6), 175.

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