Log In Sign up
The largest database of trusted experimental protocols

Nfatc1

Manufactured by BioLegend
Visit Supplier
Sourced in United States

NFATc1 is a transcription factor that plays a crucial role in regulating gene expression in various cell types, including T cells, B cells, and other immune cells. It is involved in the activation and differentiation of these cells in response to calcium signaling.

Automatically generated - may contain errors

Lab products found in correlation

Incb3284, by Cayman Chemical (2 mentions) Phospho ikba ser32, by Cell Signaling Technology (2 mentions) G 6983, by Cayman Chemical (2 mentions) Cd154, by Santa Cruz Biotechnology (2 mentions) Bay11 7082, by Enzo Life Sciences (2 mentions) T bet, by Santa Cruz Biotechnology (2 mentions) Ruvbl1, by Proteintech (2 mentions) C jun, by Abcam (2 mentions) Runx1, by Thermo Fisher Scientific (2 mentions) Bhlhe40, by Novus Biologicals (2 mentions) Snf2h, by Abcam (2 mentions) Mitotracker red cmxros, by Thermo Fisher Scientific (1 mentions) Bodipy 493 503, by Thermo Fisher Scientific (1 mentions) Bodipy fl c16, by Thermo Fisher Scientific (1 mentions) Carboxyfluorescein succinimidyl ester (cfse), by BD (1 mentions) Annexin 5, by BD (1 mentions) Ctla 4, by BioLegend (1 mentions) Fabp5, by R&D Systems (1 mentions) Pparγ, by Abcam (1 mentions) Bcl xl, by Abcam (1 mentions)

Spelling variants of this product

Anti-NFATc1 clone-7A6 (3 citations)

7 protocols using nfatc1

1

Signaling Pathways in Cell Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following chemicals were obtained: Cyclosporin A (TCI Chemical cat no.C2408), Bay11–7082 (Enzo cat no.E1279), PP2 (Tocris cat no.1407), Gö 6983 (Cayman Chemical cat no.13311), INCB3284 (Cayman Chemical cat no.1163). 200,000 cells seeded in 24 well plates were treated with/without inhibitors. Protein cell lysates were prepared and immunoblotted using procedures described24 (link). Membranes were blotted with antibodies to: CD154 (Santa Cruz Biotechnology cat no. sc978), NFATc1 (Biolegend, cat no.649601), phospho-IKBa (ser32) (Cell Signaling Technology cat no. 9246), IΚBα or ACTIN (Sigma, cat no.A5441).
Brummer G., Fang W., Smart C., Zinda B., Alissa N., Berkland C., Miller D, & Cheng N. (2019). CCR2 signaling in breast carcinoma cells promotes tumor growth and invasion by promoting CCL2 and suppressing CD154 effects on the angiogenic and immune microenvironments. Oncogene, 39(11), 2275-2289.
+ Open protocol
+ Expand
2

Multiparametric Immune Profiling of T Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were stained with CD3 (BioLegend, clone: HIT3a), CD8 (BioLegend, clone: SK1), CD45RO (BioLegend, clone: UCHL1), T-bet (BioLegend, clone: 4B10), Eomes (R&D Systems, clone: 644730), NFATC1 (BioLegend, clone: 7A6), PD-1 (BioLegend, clone: EH12.2H7), TIM-3 (BioLegend, clone: F38-2E2), CTLA4 (BioLegend, clone: L3D10), LAG3 (BioLegend, clone: 11C3C65), Bcl-XL (Abcam, clone:7B2.5), FABP5 (R&D Systems, clone: 311215)，CPT1α (Proteintech, 15184-1-AP), CD137 (BioLegend, clone: 4B4-1), mito Tracker Red CMXROS (Invitrogen, M7512), TMRM (Invitrogen, M20036), LIVE/DEAD Viability/Cytotoxicity Kit (Invitrogen, L3224), CD27 (BioLegend, clone: M-T271), CD127 (eBioscience, clone: eBioRDR5), IFNγ (BD Pharmingen, clone: B27), TCF7 (BioLegend, clone: 7F11A10), IRF4 (BioLegend, clone: IRF4.3E4), Blimp1 (BD Pharmingen, clone: 6D3), BCL2 (Cell Signaling Technology, clone: 124), Ki67 (Abcam, clone: B126.1), PPARγ (Abcam, clone: EPR18516), Annexin V (BD Pharmingen, 556547), CFSE (BD Pharmingen, 565082), KLRG1 (BioLegend, clone: 2F1/KLRG1), as well as BODIPY 493/503 (Invitrogen, D3922) and BODIPY FL C16 (Invitrogen, D3821). For intracellular staining, cells were given PMA/ionomycin (BioLegend, 423303) re-stimulation and then intracellular staining was performed as previously described.6 (link) FACS analysis was performed on a BD FACS Aria II flow cytometer and analyzed by FlowJo V.6 software.
Liu F., Liu W., Zhou S., Yang C., Tian M., Jia G., Wang H., Zhu B., Feng M., Lu Y., Qiao T., Wang X., Cao W., Wang X., Shi Y, & Wu D. (2020). Identification of FABP5 as an immunometabolic marker in human hepatocellular carcinoma. Journal for Immunotherapy of Cancer, 8(2), e000501.
+ Open protocol
+ Expand
3

Multiparametric Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following human-specific antibodies were purchased and used for flow detection, including against CD4(PE-CY7), CD27(APC), CD19(APC-CY7), CD1d(APC), IgM(FITC), CD24(PerCP/CY5.5), CD147(PE), CD25(FITC), CD71(PE), CD73(PerCP/CY5.5), CD39(FITC), TIM-1(PE), NFAT1(PE), and NFATc1(FITC), which were purchased from BioLegend, and GSK-3β(BV421), which was purchased from BD Pharmingen. Antibodies against IL-10(PE), TGF-β(FITC), GSK-3β(BV421), NFAT1(PE), and NFATc1(FITC) were used for intracellular flow cytometry assays, while the others were used for cell surface staining. Using CATON II instrument (BD Bioscience) for sufficient sample acquisition, using FlowJo VII (TreeStar) to analyze the data.
Li J., Gao J., Zhou H., Zhou J., Deng Z., Lu Y., Rao J., Ji G., Gu J., Yang X., Xia Y, & Wang X. (2020). Inhibition of Glycogen Synthase Kinase 3β Increases the Proportion and Suppressive Function of CD19+CD24hiCD27+ Breg Cells. Frontiers in Immunology, 11, 603288.
+ Open protocol
+ Expand
4

Investigating FasL-Mediated Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
FasL (ALX-522-020-C005) was purchased from Enzo Life Sciences (Farmingdale, NY, USA). LY294002 and FK506 were purchased from Cell Signaling (Beverly, MA, USA) and InvivoGen (San Diego, CA, USA), respectively. DcR3 (catalogue No. 4758; 1:1000 dilution), phosphorylated Akt Serine 473 (pAkt; catalogue No. 4060/clone D9E; 1:1000 dilution), Akt (catalogue No. 9272; 1:1000 dilution), NFATc1 (catalogue No. 8032/clone D15F1; 1:1000 dilution), pan-cadherin (catalogue No. 4073/clone 28E12; 1:1000 dilution), phosphorylated GSK-3β Serine 9 (pGSK-3β; catalogue No. 5587/clone D1A3; 1:1000 dilution) and GSK-3β (catalogue No. 9315/clone 27C10; 1:1000 dilution) antibodies were purchased from Cell Signaling (Beverly, MA, USA). GAPDH (catalogue No. SC-25778/clone FL335; 1:2000 dilution) and lamin A/C (catalogue No. MABE 481/clone EP4520; 1:2000 dilution) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA) and Millipore (Bedford, MA, USA), respectively. For immunohistochemistry, DcR3 (catalog No. 333202/clone B08-35; 1:100 dilution) and NFATc1 (catalog No. 649601/clone 7A6; 1:100 dilution) antibodies were purchased from Biolegend (San Diego, CA, USA).
Im J., Kim K., Hergert P, & Nho R.S. (2016). Idiopathic pulmonary fibrosis fibroblasts become resistant to Fas ligand-dependent apoptosis via the alteration of decoy receptor 3. The Journal of pathology, 240(1), 25-37.
+ Open protocol
+ Expand
5

CUT&RUN Antibody Profiling Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used for CUT&RUN: Runx1 (Thermo Fisher: PA5–19638), TCF1 (Cell Signaling Technology: 2203S), Ets1 (Cell Signaling Technology: 14069), T-bet (Santa Cruz Biotechnology: sc-21003 X), IRF4 (Santa Cruz Biotechnology: sc-6059), c-Jun (Abcam: ab31419), NFATc1 (Biolegend: 649607), Bhlhe40 (Novus Biologicals: NB100–1800SS), Brg1 (Abcam: ab110641), Chd4 (Abcam: ab72418), Ruvbl1 (Proteintech group: 10210–2-AP), Snf2h (Abcam: ab3749).
The following clones of fluorescently conjugated antibodies were obtained from BD Biosciences, Biolegend, Thermo Fisher, or Tonbo Bioscience and used for flow cytometry at a 1:400 dilution: CD45 (30-F11), TCRβ (H57–597), CD4 (RM4–5), CD8a (53–6.7), CD44 (IM7), CD62L (MEL-14).
Zhong Y., Walker S.K., Pritykin Y., Leslie C.S., Rudensky A.Y, & van der Veeken J. (2021). Hierarchical Regulation of the Resting and Activated T Cell Epigenome by Major Transcription Factor Families. Nature immunology, 23(1), 122-134.
+ Open protocol
+ Expand
6

CUT&RUN Antibody Profiling Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used for CUT&RUN: Runx1 (Thermo Fisher: PA5–19638), TCF1 (Cell Signaling Technology: 2203S), Ets1 (Cell Signaling Technology: 14069), T-bet (Santa Cruz Biotechnology: sc-21003 X), IRF4 (Santa Cruz Biotechnology: sc-6059), c-Jun (Abcam: ab31419), NFATc1 (Biolegend: 649607), Bhlhe40 (Novus Biologicals: NB100–1800SS), Brg1 (Abcam: ab110641), Chd4 (Abcam: ab72418), Ruvbl1 (Proteintech group: 10210–2-AP), Snf2h (Abcam: ab3749).
The following clones of fluorescently conjugated antibodies were obtained from BD Biosciences, Biolegend, Thermo Fisher, or Tonbo Bioscience and used for flow cytometry at a 1:400 dilution: CD45 (30-F11), TCRβ (H57–597), CD4 (RM4–5), CD8a (53–6.7), CD44 (IM7), CD62L (MEL-14).
Zhong Y., Walker S.K., Pritykin Y., Leslie C.S., Rudensky A.Y, & van der Veeken J. (2021). Hierarchical Regulation of the Resting and Activated T Cell Epigenome by Major Transcription Factor Families. Nature immunology, 23(1), 122-134.
+ Open protocol
+ Expand
7

Signaling Pathways in Cell Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following chemicals were obtained: Cyclosporin A (TCI Chemical cat no.C2408), Bay11–7082 (Enzo cat no.E1279), PP2 (Tocris cat no.1407), Gö 6983 (Cayman Chemical cat no.13311), INCB3284 (Cayman Chemical cat no.1163). 200,000 cells seeded in 24 well plates were treated with/without inhibitors. Protein cell lysates were prepared and immunoblotted using procedures described24 (link). Membranes were blotted with antibodies to: CD154 (Santa Cruz Biotechnology cat no. sc978), NFATc1 (Biolegend, cat no.649601), phospho-IKBa (ser32) (Cell Signaling Technology cat no. 9246), IΚBα or ACTIN (Sigma, cat no.A5441).
Brummer G., Fang W., Smart C., Zinda B., Alissa N., Berkland C., Miller D, & Cheng N. (2019). CCR2 signaling in breast carcinoma cells promotes tumor growth and invasion by promoting CCL2 and suppressing CD154 effects on the angiogenic and immune microenvironments. Oncogene, 39(11), 2275-2289.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!