The blood of mice was harvested at indicated time points after the CCL4 injection. The blood was placed at 4℃ overnight, followed by centrifuging to obtain serum. Then, the concentrations of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the obtained serum were detected by using an automatic biochemical analyzer (Hitachi High-Technologies Crop., Minato-ku, Tokyo, Japan).
Automatic biochemical analyzer
Manufactured by Hitachi
Sourced in Japan, China
The Automatic Biochemical Analyzer is a laboratory instrument designed to automatically perform biochemical analyses on various samples. It is capable of processing multiple samples simultaneously and providing quantitative measurements of specific analytes, such as enzymes, proteins, and metabolites, in the samples.
Automatically generated - may contain errors
Lab products found in correlation
Blood glucose meter, by Bayer (2 mentions)
Complement c3 and c4 kits, by Beckman Coulter (1 mentions)
Microplate reader, by Bio-Rad (1 mentions)
Insulin elisa kit, by Solarbio (1 mentions)
Glucose content assay kit, by Applygen (1 mentions)
Bca kit, by Beyotime (1 mentions)
Rat crp elisa kit, by Merck Group (1 mentions)
Xe 5000, by Sysmex (1 mentions)
Glucometer, by Roche (1 mentions)
Msp 2s, by Hitachi (1 mentions)
Ht7700, by Hitachi (1 mentions)
Su8100, by Hitachi (1 mentions)
Hydrogen peroxide assay kit, by NJJCBIO (1 mentions)
Coulter stks, by Beckman Coulter (1 mentions)
D glucose, by Merck Group (1 mentions)
Insulin, by Roche (1 mentions)
Primus ultra 2, by Trinity Biotech (1 mentions)
Urine protein test kit, by Nanjing Jiancheng (1 mentions)
Turbidimetric immunoassay, by Hitachi (1 mentions)
Quantichrom creatinine assay kit, by BioAssay Systems (1 mentions)
128 protocols using automatic biochemical analyzer
1
Hepatic Injury Marker Quantification
2
Serum Biomarker Measurement Protocol
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The levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), and total cholesterol (TC) were measured using an automatic biochemical analyzer (Hitachi High-Technologies Corporation, Tokyo, Japan). The concentration of LPS in serum was measured with the Endpoint Chromogenic Endotoxin Detection LAL Kit.
3
Autoimmune Antibody and Complement Assays
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ANA and anti-ds-DNA antibody kits were purchased from Trinity Biotech, Inc. (Jamestown, NY, USA); Microplate reader was purchased from Bio-Rad Laboratories, Inc. (Hercules, CA, USA); complement C3 and C4 kits were purchased from Beckman Coulter, Inc. (Brea, CA, USA); Hitachi automatic biochemical analyzer was purchased from Hitachi, Ltd. (Tokyo, Japan).
4
Comprehensive Biochemical Analysis of Plasma in Study
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The plasma total free fatty acid (FFA) was measured by enzyme calorimetry using an assay kit (Waco Chemicals, Waco, TX) (Zhu et al., 2010 (link)). The plasma triglyceride and cholesterol levels were analyzed by the Boehringer Mannheim/Hitachi 912 analyzer (Roch Diagnostics, Indianapolis, IN) according to a previous description (Long et al., 2007 ). The 3-hydroxybutyrate dehydrogenase was used to measure the plasma BHB using spectrophotometry (Wu et al., 1991 ). The levels of plasma alkaline phosphatase (AKP), albumin (ALB), total protein (TP), alanine transaminase (ALT) and aspartate transaminase (AST) were measured by the Hitachi automatic biochemical analyzer (7600-020, Hitachi Ltd., Tokyo, Japan) using the commercially available AKP (060701, Prodia diagnostics, Germany), ALB (20110622, Kehua Biological Cor. Shanghai, China), TP (20110422, KehuaBiological Cor. Shanghai, China), ALT (050801, Prodia diagnostics, Germany), and AST (070201, Prodia diagnostics, Germany) detection kits, respectively. The globulin (GLB) content was determined as the difference between TP and ALB. The specific commercial ELISA kits (CUSABIO; MEIMIAN) were used to measure the serum amyloid protein A (SAA) and paraoxonase (PON) levels.
5
Evaluating Intraportal Oridonin Hepatotoxicity
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To evaluate potential hepatotoxicity caused by intraportal oridonin, serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) were tested by using the Hitachi automatic biochemical analyzer (cat. no. 7600; HANGZHOU RUIXI TECHNOLOGY CO., LTD., Hangzhou, China).
6
Bovine Serum Biochemical and Antioxidant Analysis
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At the end of this trial, blood samples from each steer were collected from the coccygeal vessels before the morning feeding into two 10 mL vacutainer tubes (one for blood biochemical analysis and another for serum metabolomics analysis) and then centrifuged at 4000 r/min for 15 min at 4 °C to prepare serum. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), globulin (GLB), total protein (TP), albumin (ALB), urea, total bilirubin (TBIL), blood glucose (GLU), total cholesterol (TC), and triglycerides (TGs) were determined according to the instructions of the kit (Nanjing Jiancheng Institute of Bioengineering, Nanjing, China) using a Hitachi automatic biochemical analyzer (model 7600, Hitachi, Japan). Serum antioxidant indices, including total antioxidant capacity (T-AOC) and malondialdehyde (MDA), were determined using the colorimetric method according to the kit instructions (Nanjing Jiancheng Institute of Bioengineering, Nanjing, China).
7
Metabolic Profile Evaluation Protocol
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Serum levels of alanine aminotransferase (ALT), total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) and fasting blood glucose (FBG) were measured by a Hitachi automatic biochemical analyzer (Hitachi, Tokyo, Japan). Fasting blood insulin (FBI) was assessed by an insulin ELISA kit (Solarbio, Beijing, China). Homeostasis model assessment of insulin resistance (HOMA-IR) was calculated by the formula “HOMA-IR=FBI×FBG/22.5”.
8
Serum Cyr61 and Inflammatory Markers
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Serum Cyr61 levels were measured using a commercially available ELISA kit (DRG Diagnostics, Marburg, Germany) according to the manufacturer's instruction. Blood glucose, lipids and other related indicators were also detected simultaneously with a Hitachi Automatic Biochemical Analyzer (Hitachi Co., Tokyo, Japan). Serum high sensitivity C-reactive protein (hs-CRP) levels were measured with the commercial ELISA kit (Dade Behring Diagnostics GmbH, Germany).
9
Glucose Uptake Assay in L6 Myotubes
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L6 cells were seeded in a 96-well transparent plate with low density in 100 µL DMEM. After 6 days of differentiation, as described above. L6 myotubes cells were treated with B4 (64, 16, 4 μg/mL), and PBS buffer dissolved in 100 μL DMEM medium, six wells were repeated in each group. After 24 h of treatment, the cell supernatant was taken, and the glucose concentration was detected by the glucose oxidase method using an automatic biochemical analyzer (HITACHI, Japan). Glucose consumption in the culture medium reflects intracellular glucose uptake.
L6 cells were cultured in a 6-well transparent plate. There were four wells in the normal group and the B4-treated groups. After B4 (64, 16, 4 μg/mL) treatment for 24 h, cells were collected and the intracellular glucose was lysed according to the instructions of the glucose content assay kit (APPLYGEN, Beijing, China), the supernatant of lysate was used to measure the glucose content using the oxidase method, and the protein in the precipitate was detected by a BCA kit (Beyotime, Beijing, China). The amount of glucose per gram of protein was calculated and deemed as glucose uptake.
L6 cells were cultured in a 6-well transparent plate. There were four wells in the normal group and the B4-treated groups. After B4 (64, 16, 4 μg/mL) treatment for 24 h, cells were collected and the intracellular glucose was lysed according to the instructions of the glucose content assay kit (APPLYGEN, Beijing, China), the supernatant of lysate was used to measure the glucose content using the oxidase method, and the protein in the precipitate was detected by a BCA kit (Beyotime, Beijing, China). The amount of glucose per gram of protein was calculated and deemed as glucose uptake.
10
Serum Biomarker Levels in Rats
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The rat serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL), and mitochondrial AST (ASTm) were measured using an automatic biochemical analyzer (Hitachi, Japan) according to the manufacturer’s instructions.
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