Atplite

Manufactured by PerkinElmer

Sourced in United States, United Kingdom, Italy

The ATPlite is a luminescence-based assay kit designed to quantify the presence of ATP, a universal energy currency in living cells. It provides a rapid and sensitive method for detecting ATP levels in a variety of sample types.

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Lab products found in correlation

Viewlux plate reader, by PerkinElmer (9 mentions) Victor multilabel counter, by PerkinElmer (5 mentions) Victor plate reader, by PerkinElmer (4 mentions) El406, by Agilent Technologies (3 mentions) Viewplate, by PerkinElmer (3 mentions) Spectramax i3x platform, by Molecular Devices (3 mentions) Luminoskan ascent, by Thermo Fisher Scientific (3 mentions) Spectramax m5, by Molecular Devices (2 mentions) Atplite, by Bio-Rad (2 mentions) Infinite m1000 pro multilabel microplate reader, by PerkinElmer (2 mentions) Tc20 automated cell counter, by Bio-Rad (2 mentions) 384 well microtiter plates, by Thermo Fisher Scientific (2 mentions) Caspase glo 3 7 assay, by Promega (2 mentions) Enspire, by PerkinElmer (2 mentions) Dc protein assay kit, by Bio-Rad (2 mentions) Enspire multilabel reader, by PerkinElmer (2 mentions) Victorx4 reader, by PerkinElmer (2 mentions) Tristar lb 941, by Berthold Technologies (2 mentions) Mtt assay, by Merck Group (2 mentions) Ripa buffer, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

ATPlite kit (89 citations) ATPlite Luminescence ATP Detection Assay System (56 citations) ATPlite assay kit (45 citations) ATPlite luminescence assay kit (37 citations) ATPlite luminescence assay (26 citations) ATPlite 1step assay (9 citations) ATPlite luminescence-based assay (8 citations) ATPLite luminescent assay (5 citations) ATPLite Luminescence Assay System kit (4 citations) ATPlite Luminiescent Assay kit (4 citations)

146 protocols using atplite

Quantifying metabolic activity and proliferation of 2D and 3D cancer cell cultures

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Metabolic activity of 2D and 3D MDA-MB-231 cell cultures was assessed with CellTiter-Glo® cell viability reagent (Promega, #G7570) according to the manufacturer's instructions without any modification: the diluted CellTiter Glo substrate was added to the culture wells at the end of the treatment period, followed by a 10 min incubation, and luminescence quantification on a FLUOstar Optima (BMG labtech).
The Adenosine TriPhosphate (ATP)-monitoring luminescence assay ATPLite TM (PerkinElmer, #6016943) was used to quantitatively assess proliferation and cytotoxicity of 3D MCF-7 cultures. The ATPLite TM assay followed standard recommended no wash one step protocol and was read on an EnSight TM Multimode Plate Reader (PerkinElmer), without any deviation from the manufacturers protocol: 50 μL cell lysis solution was added to the 3D cell cultures and incubated for 5 minutes on an orbital shaker, followed by the addition of 50 μL ATPLite substrate solution and a further 5-minute shaking. The culture plates were then incubated for 10 minutes in the dark, before the quantification of their luminescence intensity.

Engel M., Belfiore L., Aghaei B, & Sutija M. (2022). Enabling high throughput drug discovery in 3D cell cultures through a novel bioprinting workflow. SLAS technology, 27(1).

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Measuring ATP Secretion in Enteric Glial Cells

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Luciferin-luciferase assay was used to monitor basal secretion of ATP according to the manufacturer’s instructions (ATP-lite, Perkin Elmer) using 100μl of the supernatant.
EGCs were grown in 12-well dishes (2×10⁴ cells in each well) in DMEM supplemented with 10% FBS and 1% penicillin-streptomycin until confluence was reached (7–10 days). Cell cultures were grown individually from 4 different surgical specimens and were used at passages 4–7. EGCs isolation was performed from MP of 3 surgical patients (2 jejunum, 1 colon) and SMP of one patient (colon). Preliminary analysis did not reveal any differences in amount of ATP secretion in each surgical specimen and therefore, data in different surgical specimens were pooled together. To study the effect of treatment on ATP secretion, cells were incubated with LPS+IFNγ in 400μl of DMEM with 10% FBS and 1% penicillin-streptomycin. For controls the medium alone was used. Supernatants (300μl) were collected and immediately frozen in liquid nitrogen for measurement of ATP (ATPlite, Perkin Elmer).

Liñán-Rico A., Turco F., Ochoa-Cortes F., Harzman A., Needleman B.J., Arsenescu R., Abdel-Rasoul M., Fadda P., Grants I., Whitaker E., Cuomo R, & Christofi F.L. (2016). Molecular Signaling and Dysfunction of the Human Reactive Enteric Glial Cell Phenotype: Implications for GI infection, IBD, POI, Neurological, Motility and GI Disorders. Inflammatory bowel diseases, 22(8), 1812-1834.

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Evaluating Safety of Ophthalmic Compounds

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Adult retinal pigment epithelial cells (ARPE-19; American Type Culture Collection, Manassas, VA) were cultured at 37°C (humidified atmosphere with 5% CO₂) in Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (DMEM:F12) medium (30-2006; American Type Culture Collection) and 100 U/mL penicillin, 100 μg/mL streptomycin, and 10% fetal bovine serum. The study was divided into two phases:

Phase I—Evaluation of the safety profiles of single-compound (LB-VD and TA) formulations on ARPE-19 cells with the following endpoints: cell morphology evaluation by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Chemicon International, Temecula, CA), lactate dehydrogenase (LDH) release assay, and ATPlite (PerkinElmer, Waltham, MA) after 1, 5, and 30 minutes of treatment with formulations

Phase II—Evaluation of the safety profiles of the same formulations in combination with ultrapure PFD, with the following endpoints: MTT and ATPlite after 1 and 5 minutes of treatment with the formulations and 24 hours of treatment with PFD. We also attempted to evaluate the LDH release; however, we were not able to detect LDH, probably due to a strong interference with PFD in the medium.

Lazzara F., Conti F., Ferrara M., Lippera M., Coppola M., Rossi S., Drago F., Bucolo C, & Romano M.R. (2023). Safety Profile of Lutein- Versus Triamcinolone Acetonide–Based Vitreous Staining. Translational Vision Science & Technology, 12(1), 5.

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Melanoma Cell Growth Inhibition Assay

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Cell growth was assayed using ATPlite (Perkin Elmer). Briefly, melanoma cells (5,000 cells/well) were cultured in 96-well plates with a transparent bottom. Twenty-four hours after seeding, inhibitors were added for 72 or 120 hr. Cell growth or viability was measured using ATPlite (Perkin Elmer) and quantified by monitoring luminescence intensity.

Kim H., Frederick D.T., Levesque M.P., Cooper Z.A., Feng Y., Krepler C., Brill L., Samuels Y., Hayward N.K., Perlina A., Piris A., Zhang T., Halaban R., Herlyn M.M., Brown K.M., Wargo J.A., Dummer R., Flaherty K.T, & Ronai Z.A. (2015). Downregulation of the Ubiquitin Ligase RNF125 Underlies Resistance of Melanoma Cells to BRAF Inhibitors via JAK1 Deregulation. Cell reports, 11(9), 1458-1473.

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