Gtx127345

Manufactured by GeneTex

Sourced in United States, Germany

GTX127345 is a precision laboratory centrifuge designed for efficient separation of biological samples. It features a high-speed motor and adjustable rotor for versatile applications. The centrifuge operates at controlled temperatures to maintain sample integrity during processing.

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Lab products found in correlation

Deferoxamine dfo d9533, by Merck Group (1 mentions) Mitoq, by Abcam (1 mentions) Ferrostatin 1, by Merck Group (1 mentions) Epr3776, by Abcam (1 mentions) Rhodamine conjugated anti mouse igg, by Jackson ImmunoResearch (1 mentions) Sc 9996, by Santa Cruz Biotechnology (1 mentions) E cadherin, by Cell Signaling Technology (1 mentions) Ab49254, by Abcam (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Gtx100619, by GeneTex (1 mentions) Fitc conjugated anti rabbit igg, by Jackson ImmunoResearch (1 mentions) Gtx112794, by GeneTex (1 mentions) Gtx128508, by GeneTex (1 mentions) Alkaline phosphatase conjugated anti rabbit igg antibody, by Jackson ImmunoResearch (1 mentions) Sc 7269, by Santa Cruz Biotechnology (1 mentions) N cadherin, by GeneTex (1 mentions) Tgf β1, by Thermo Fisher Scientific (1 mentions) Anti gapdh gtx627408, by GeneTex (1 mentions) Gtx111643, by GeneTex (1 mentions) Gtx26308, by GeneTex (1 mentions)

4 protocols using gtx127345

Antibodies and Inhibitors in Cell Research

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The antibodies used were as follows: rabbit antibody against human E-cadherin (24E10; Cell Signaling Technology, Danvers, MA, United States); rabbit antibody against human N-cadherin (GTX127345; GENETEX, Inc., Irvine, CA, United States); rabbit antibody against human vimentin (EPR3776; Abcam, Cambridge, United Kingdom); rabbit antibody against human β-actin (4967; Bioss Antibodies, Woburn, MA, United States); and HRP-conjugated secondary antibody against rabbit IgG (7074; Cell Signaling Technology). The inhibitors used were as follows: deferoxamine (DFO; D9533; Sigma-Aldrich, St. Louis, MO, United States), ferrostatin-1 (SML0583; Sigma-Aldrich), and MitoQ (B1309; Biovision, Milpitas, CA, United States).

Takahashi M., Mizumura K., Gon Y., Shimizu T., Kozu Y., Shikano S., Iida Y., Hikichi M., Okamoto S., Tsuya K., Fukuda A., Yamada S., Soda K., Hashimoto S, & Maruoka S. (2022). Iron-Dependent Mitochondrial Dysfunction Contributes to the Pathogenesis of Pulmonary Fibrosis. Frontiers in Pharmacology, 12, 643980.

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Western Blotting and Immunofluorescence Staining Protocols

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Western blotting staining and immunohistochemical (fluorescence) staining were performed as described previously 17, 19. The primary antibodies used in this study were Snail (1:1,000 dilution; MABE167; Merck, Darmstadt, Germany), E‐cadherin (1:1,000 dilution; #3195S; Cell Signaling Technology), PCAF (1:1,000 dilution; #3378S; Cell Signaling Technology), vimentin (1:2,000 dilution; GTX100619; GeneTex), β‐actin (1:10,000 dilution; #4967L; Cell Signaling Technology), HA (1:1,000 dilution; #3724S; Cell Signaling Technology), GFP (1:500 dilution; SC‐9996; Santa Cruz Biotechnology), ISX (1:200 dilution; sc‐398934; Santa Cruz Biotechnology), TWIST1 (1:200 dilution; ab49254; Abcam), BRD4 (1:1,000 dilution; #13440S; Cell Signaling Technology), acetylated lysine (1:500 dilution; #9441S Cell Signaling Technology), fibronectin (1:500 dilution; GTX112794; GeneTex), mCherry (1:500 dilution; GTX128508; GeneTex), Slug (1:1,000 dilution; GTX128796; GeneTex), VEGF (1:200 dilution; sc‐7269; Santa Cruz Biotechnology), and N‐cadherin (1:1,000 dilution; GTX127345; GeneTex). FITC‐conjugated anti‐rabbit IgG, rhodamine‐conjugated anti‐mouse IgG, and alkaline phosphatase‐conjugated anti‐rabbit IgG antibody (1:500 dilution; Jackson ImmunoResearch Laboratories, West Grove, PA, USA) were also used. All experiments were repeated at least three times.

Wang L., Liu K., Jeng W., Chiang C., Chai C., Chiou S., Huang M., Yokoyama K.K., Wang S., Huang S, & Hsu S. (2020). PCAF‐mediated acetylation of ISX recruits BRD4 to promote epithelial‐mesenchymal transition. EMBO Reports, 21(2), e48795.

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Extracellular Vesicle Protein Detection

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TGFβ1 was purchased from Peprotech (Rocky Hill, NJ). The primary antibodies were used at 1:1000 ratio. Monoclonal anti-CD63 (H5C6, The Developmental Studies Hybridoma Bank, Iowa City, IA), anti-CD81 (MAB 4615, R&D Systems, Minneapolis, MN), anti-Thy-1.2 (HO-13-4, ATCC and 550402, BD Biosciences, San Diego, CA), anti-GAPDH (GTX627408, GeneTex, Irvine, CA) and rabbit polyclonal anti-calnexin (2679S, Cell Signaling, Danvers, MA) were used to detect EV and cellular proteins. Monoclonal anti-FN-EDA (ab6328, Abcam, Cambridge, MA), monoclonal anti-collagen I (GTX26308, GeneTex) and rabbit polyclonal anti-α-SMA (CBL171-I, EMD Millipore, Temecula, CA) anti-collagen III (GTX111643, GeneTex) and anti-N-cadherin (GTX127345, GeneTex) were used in western blotting studies.

Shentu T.P., Huang T.S., Cernelc-Kohan M., Chan J., Wong S.S., Espinoza C.R., Tan C., Gramaglia I., van der Heyde H., Chien S, & Hagood J.S. (2017). Thy-1 dependent uptake of mesenchymal stem cell-derived extracellular vesicles blocks myofibroblastic differentiation. Scientific Reports, 7, 18052.

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Bronchial Epithelial Cell Culture Protocol

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Normal human bronchial epithelial cells (NHBE, Lonza) were maintained in Keratinocyte SFM basal medium supplemented with 5 μg/L human recombinant epithelial growth factor (EGF), 50 mg/L bovine pituitary extract (BPE), 5 mg/L insulin, and 25 nM hydrocortisone at 37°C in a 5% CO₂ atmosphere. Cells were passaged every 3 days and plated for experimental treatment within 6 passages.
NaAsO₂, montelukast, fluticasone, N-acetylcysteine, and BAY117082 were purchased from Sigma–Aldrich (United States). Antibodies against fibronectin (Sigma, F3648), MMP-2 (GeneTex, GTX 104577), GAPDH (GeneTex, GTX100118), SMAD2/3 (GeneTex, GTX111123), β-catenin (R&D, AF1329), N-Cadherin (GeneTex, GTX127345), and E-Cadherin (GeneTex, GTX100443) were used.

Chen H.C., Chiou H.Y., Tsai M.L., Chen S.C., Lin M.H., Chuang T.C., Hung C.H, & Kuo C.H. (2022). Effects of Montelukast on Arsenic-Induced Epithelial-Mesenchymal Transition and the Role of Reactive Oxygen Species Production in Human Bronchial Epithelial Cells. Frontiers in Pharmacology, 13, 877125.

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