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Goat anti mouse rabbit igg

Manufactured by Southern Biotech
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Goat anti-mouse/rabbit IgG is a secondary antibody that binds to primary antibodies raised in mouse or rabbit. It is commonly used in various immunological techniques, such as Western blotting, ELISA, and immunohistochemistry, to detect and quantify target proteins.

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Lab products found in correlation

Alkaline phosphatase, by Jackson ImmunoResearch (2 mentions) Maxisorp, by Thermo Fisher Scientific (2 mentions) A microplate reader, by Agilent Technologies (1 mentions) Microplate reader, by Agilent Technologies (1 mentions)

2 protocols using goat anti mouse rabbit igg

1

SARS-CoV-2 RBD Binding Inhibition Assay

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SARS-CoV-2 mouse/rabbit Fc-tagged RBDs (final concentration 20 ng/ml) were incubated with serially diluted recombinant mAbs (from 25 µg/ml) and incubated for 1 h 37°C. The complex RBD:mAbs was then added to a pre-coated hACE2 (2 µg/ml in PBS) 96-well plate MaxiSorp (Nunc) and incubated 1 hour at room temperature. Subsequently, the plates were washed, and a goat anti-mouse/rabbit IgG (Southern Biotech) coupled to alkaline phosphatase (Jackson Immunoresearch) added to detect mouse Fc-tagged RBDs binding. After further washing, the substrate (p-NPP, Sigma) was added, and plates read at 405 nm using a microplate reader (Biotek). The percentage of inhibition was calculated as follow: (1-((OD sample-OD neg ctr)/(OD pos. ctr-OD neg. ctr))*100
Park Y.J., Pinto D., Walls A.C., Liu Z., Marco A.D., Benigni F., Zatta F., Silacci-Fregni C., Bassi J., Sprouse K.R., Addetia A., Bowen J.E., Stewart C., Giurdanella M., Saliba C., Guarino B., Schmid M.A., Franko N., Logue J., Dang H.V., Hauser K., Iulio J.D., Rivera W., Schnell G., Rajesh A., Zhou J., Farhat N., Kaiser H., Montiel-Ruiz M., Noack J., Lempp F.A., Janer J., Abdelnabi R., Maes P., Ferrari P., Ceschi A., Giannini O., de Melo G.D., Kergoat L., Bourhy H., Neyts J., Soriaga L., Purcell L.A., Snell G., Whelan S.P., Lanzavecchia A., Virgin H.W., Piccoli L., Chu H., Pizzuto M.S., Corti D, & Veesler D. (2022). Imprinted antibody responses against SARS-CoV-2 Omicron sublineages. bioRxiv.
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2

SARS-CoV-2 RBD-ACE2 Binding Inhibition Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
SARS-CoV and SARS-CoV-2 mouse/rabbit Fc-tagged RBDs (final concentration 20 ng/ml) were incubated with serially diluted recombinant mAbs (from 25 μg/ml) and incubated for 1 h 37°C. The complex RBD:mAbs was then added to a pre-coated hACE2 (2 μg/ml in PBS) 96-well plate MaxiSorp (Nunc) and incubated 1 hour at room temperature. Subsequently, the plates were washed and a goat anti-mouse/rabbit IgG (Southern Biotech) coupled to alkaline phosphatase (Jackson Immunoresearch) added to detect mouse Fc-tagged RBDs binding. After further washing, the substrate (p-NPP, Sigma) was added, and plates read at 405 nm using a microplate reader (Biotek). The percentage of inhibition was calculated as follow: (1-((OD sample-OD neg ctr)/(OD pos. ctr-OD neg. ctr))*100
Park Y.J., De Marco A., Starr T.N., Liu Z., Pinto D., Walls A.C., Zatta F., Zepeda S.K., Bowen J., Sprouse K.S., Joshi A., Giurdanella M., Guarino B., Noack J., Abdelnabi R., Foo S.Y., Lempp F.A., Benigni F., Snell G., Neyts J., Whelan S.P., Virgin H.W., Bloom J.D., Corti D., Pizzuto M.S, & Veesler D. (2021). Antibody-mediated broad sarbecovirus neutralization through ACE2 molecular mimicry. bioRxiv.
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