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Vetscan vs2 chemistry analyzer

Manufactured by Abaxis
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Sourced in United States, United Kingdom

The VetScan VS2 Chemistry Analyzer is a compact, self-contained laboratory instrument designed for the analysis of a variety of clinical chemistry tests. The analyzer utilizes optical technology to perform these tests on small sample volumes, providing rapid results to support veterinary healthcare professionals.

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Lab products found in correlation

Procyte dx, by IDEXX (6 mentions) Vetscan comprehensive diagnostic profile reagent rotor, by Abaxis (4 mentions) Vetscan hm5 hematology analyzer, by Abaxis (3 mentions) Comprehensive diagnostic profile rotor for geriatric testing, by Abaxis (2 mentions) Lithium heparin coated tubes, by Abaxis (2 mentions) Comprehensive diagnostic profile rotor, by Abaxis (2 mentions) Bioplex 200 mouse cytokine array chemokine array 32 plex, by Eve Technologies (2 mentions) Coag dx analyzer, by IDEXX (1 mentions) Vetscan comprehensive diagnostic profile, by Abaxis (1 mentions) Serum separator sst microtainers, by BD (1 mentions) Ast elisa kit, by Cloud-Clone (1 mentions) C57bl 6j, by Charles River Laboratories (1 mentions) Hemavet multispecies hematology analyzer, by Drew Scientific (1 mentions) Vetscan comprehensive diagnostic profile rotors, by Abaxis (1 mentions) Hm5 hematology system, by Abaxis (1 mentions) Mammalian liver profile, by Abaxis (1 mentions)

31 protocols using vetscan vs2 chemistry analyzer

1

Comprehensive Blood Analysis Protocol

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On Days 0 (pre-challenge), 5, 10, 14, 21, and 28, blood was collected from the anterior vena cava into serum separator tubes and tubes containing anticoagulant (ethylenediaminetetraacetic acid [EDTA] and sodium citrate) for clinical chemistry, hematology, and coagulation analyses. Additional terminal collections were performed on animals that required euthanasia due to advanced clinical disease. Clinical chemistry was performed on harvested serum using Abaxis VetScan Comprehensive Diagnostic Profile reagent rotors (Abaxis, Inc., Union City, CA, USA) in conjunction with the Abaxis VetScan VS2® Chemistry Analyzer (Abaxis, Inc.). Hematology was performed on EDTA blood using the Abaxis VETSCAN® HM5 Hematology Analyzer (Abaxis, Inc.). Sodium citrate blood was analyzed for Activated Partial Thromboplastin Time (aPTT) and Prothrombin Time (PT) using the IDEXX Coag Dx™ Analyzer (IDEXX Laboratories, Inc., Westbrook, ME, USA) and associated aPTT/PT test cartridges. All clinical pathology instrumentation was operated per manufacturer instructions.
Brasel T., Comer J.E., Massey S., Smith J., Smith J., Hyde M., Kocsis A., Gainey M., Niemuth N., Triplett C, & Rudge T J.r. (2021). Mucosal Challenge Ferret Models of Ebola Virus Disease. Pathogens, 10(3), 292.
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2

Serum Biochemistry Analysis Protocol

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For serum biochemistry, terminal cardiac puncture was used to collect blood into serum separator SST microtainers (BD Biosciences, San Jose, CA). Sample tubes were inverted 8-10 times immediately after collection and held at room temperature for 30 min prior to centrifugation at 1200 × g for 10 min. Serum was collected and immediately stored at -80 °C until use. All samples that contained significant hemolysis were excluded. Serum metabolites and enzymes were measured using both the VetScan Comprehensive Diagnostic Profile and Mammalian Liver Profile reagent rotors (Abaxis, Union City, CA) on the Abaxis VetScan VS2 chemistry analyzer (Abaxis, Union City, CA) following the manufacturer's instructions. Amino aspartate transferase (AST) levels in serum were measured with the AST ELISA kit (Cloud-Clone Corp., Wuhan China) following the manufacturer's instructions.
Yu F.P.S., Molino S., Sikora J., Rasmussen S., Rybova J., Tate E., Geurts A.M., Turner P.V., Mckillop W.M, & Medin J.A. (2019). Hepatic pathology and altered gene transcription in a murine model of acid ceramidase deficiency. Laboratory investigation; a journal of technical methods and pathology, 99(10).
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3

Serum Biochemistry Profile Analysis

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Serum samples were tested for concentrations of albumin, amylase, alanine aminotransferase, total bilirubin, alkaline phosphatase, glucose, total protein, blood urea nitrogen, creatinine, phosphorus, calcium (Ca2+), sodium (Na+), potassium (K+), and globulin by using a VetScan VS2 Chemistry Analyzer with comprehensive Diagnostic Profile Reagent Rotor Package (Abaxis).
Gilchuk P., Kuzmina N., Ilinykh P.A., Huang K., Gunn B.M., Bryan A., Davidson E., Doranz B.J., Turner H.L., Fusco M.L., Bramble M.S., Hoff N.A., Binshtein E., Kose N., Flyak A.I., Flinko R., Orlandi C., Carnahan R., Parrish E.H., Sevy A.M., Bombardi R.G., Singh P.K., Mukadi P., Muyembe-Tamfum J.J., Ohi M.D., Saphire E.O., Lewis G.K., Alter G., Ward A.B., Rimoin A.W., Bukreyev A, & Crowe JE J.r. (2018). Multifunctional Pan-ebolavirus Antibody Recognizes a Site of Broad Vulnerability on the Ebolavirus Glycoprotein. Immunity, 49(2), 363-374.e10.
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4

Whole Blood Analysis of Mice

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Whole blood was collected into lithium-heparin coated tubes (Abaxis, UK) from the carotid artery following cervical dislocation of mice. 120-140 μL of whole blood was then added into either the comprehensive diagnostic profile rotor for geriatric testing (Abaxis, UK) and ran on the VetScan VS2 Chemistry Analyzer (Abaxis, UK).
Guerrero A., Herranz N., Sun B., Wagner V., Gallage S., Guiho R., Wolter K., Pombo J., Irvine E.E., Innes A.J., Birch J., Glegola J., Manshaei S., Heide D., Dharmalingam G., Harbig J., Olona A., Behmoaras J., Dauch D., Uren A.G., Zender L., Vernia S., Martínez-Barbera J.P., Heikenwalder M., Withers D.J, & Gil J. (2019). Cardiac glycosides are broad-spectrum senolytics. Nature metabolism, 1(11), 1074-1088.
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5

Whole Blood Analysis of Mice

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Whole blood was collected into lithium-heparin coated tubes (Abaxis, UK) from the carotid artery following cervical dislocation of mice. 120-140 μL of whole blood was then added into either the comprehensive diagnostic profile rotor for geriatric testing (Abaxis, UK) and ran on the VetScan VS2 Chemistry Analyzer (Abaxis, UK).
Guerrero A., Herranz N., Sun B., Wagner V., Gallage S., Guiho R., Wolter K., Pombo J., Irvine E.E., Innes A.J., Birch J., Glegola J., Manshaei S., Heide D., Dharmalingam G., Harbig J., Olona A., Behmoaras J., Dauch D., Uren A.G., Zender L., Vernia S., Martínez-Barbera J.P., Heikenwalder M., Withers D.J, & Gil J. (2019). Cardiac glycosides are broad-spectrum senolytics. Nature metabolism, 1(11), 1074-1088.
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6

Avian Hematology and Chemistry Analysis

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Blood (6–12 mL) was collected from the cervical sinus and preserved in lithium heparin. Blood films were made and stained using Dif Quik (Siemens, Munich, Germany), and centrifugally spun hematocrit was obtained using a hematocrit tube from the collected sample immediately after collection. Manual white blood cell counts were obtained using Leukopet WBC (Vetlab Supply, Palmetto Bay, Florida). Differentials were calculated after preparing the solution by mixing the collected blood sample with the stain and allowing the mixture to sit for the required amount of time. Heterophils and eosinophils were counted in both chambers of a Neubauer hemacytometer under the 10× objective to determine total leukocyte count. A chemistry panel was run on an Abaxis VetScan VS2 chemistry analyzer (Abaxis, Union City, California) within 1 h after collection using an Avian/Reptilian Profile Plus cartridge (Covetrus, Portland, Maine).
Greene W., Chan B., Bromage E., Grose J.H., Walsh C., Kortright K., Forrest S., Perry G., Byrd L, & Stamper M.A. (2021). The Use of Bacteriophages and Immunological Monitoring for the Treatment of a Case of Chronic Septicemic Cutaneous Ulcerative Disease in a Loggerhead Sea Turtle Caretta caretta. Journal of Aquatic Animal Health, 33(3), 139-154.
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7

Serum ALT Quantification Protocol

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Blood was collected into nonanticoagulant, clot activator tubes and spun down at 1860g for 10 min to separate the serum from the clotted blood. Serum (100 μl) was assessed by the Abaxis Vetscan VS2 Chemistry Analyzer for the quantitative analysis of ALT.
Ricciardi M.J., Rust L.N., Pedreño-Lopez N., Yusova S., Biswas S., Webb G.M., Gonzalez-Nieto L., Voigt T.B., Louw J.J., Laurino F.D., DiBello J.R., Raué H.P., Barber-Axthelm A.M., Chun K., Uttke S., Raphael L.M., Yrizarry-Medina A., Rosen B.C., Agnor R., Gao L., Labriola C., Axthelm M., Smedley J., Julander J.G., Bonaldo M.C., Walker L.M., Messaoudi I., Slifka M.K., Burton D.R., Kallas E.G., Sacha J.B., Watkins D.I, & Burwitz B.J. (2023). Therapeutic neutralizing monoclonal antibody administration protects against lethal yellow fever virus infection. Science translational medicine, 15(689), eade5795.
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8

Serum ALT Quantification Protocol

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Blood was collected into nonanticoagulant, clot activator tubes and spun down at 1860g for 10 min to separate the serum from the clotted blood. Serum (100 μl) was assessed by the Abaxis Vetscan VS2 Chemistry Analyzer for the quantitative analysis of ALT.
Ricciardi M.J., Rust L.N., Pedreño-Lopez N., Yusova S., Biswas S., Webb G.M., Gonzalez-Nieto L., Voigt T.B., Louw J.J., Laurino F.D., DiBello J.R., Raué H.P., Barber-Axthelm A.M., Chun K., Uttke S., Raphael L.M., Yrizarry-Medina A., Rosen B.C., Agnor R., Gao L., Labriola C., Axthelm M., Smedley J., Julander J.G., Bonaldo M.C., Walker L.M., Messaoudi I., Slifka M.K., Burton D.R., Kallas E.G., Sacha J.B., Watkins D.I, & Burwitz B.J. (2023). Therapeutic neutralizing monoclonal antibody administration protects against lethal yellow fever virus infection. Science translational medicine, 15(689), eade5795.
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9

Routine Liver Function Profiling in Mice

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Mice were anesthetized and 100 μL of whole blood was collected via retro-orbital bleeding in lithium heparin blood collection tubes and transferred to single use VetScan mammalian liver profile reagent rotors. The levels of multiple analytes, including alkaline phosphatase (ALP), alanine aminotransferase (ALT), gamma glutamyl transferase (GGT), bile acids (BA), total bilirubin (TBIL), albumin (ALB), blood urea nitrogen (BUN), total cholesterol (CHOL) were quantified using a VetScan VS2 Chemistry Analyzer (Abaxis North America, USA).
Veeriah V., Lee S.H, & Levine F. (2022). Long-term oral administration of an HNF4α agonist prevents weight gain and hepatic steatosis by promoting increased mitochondrial mass and function. Cell Death & Disease, 13(1), 89.
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10

Hematology and Serum Chemistry Evaluation

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Hematology analysis was completed on a ProCyte DX (IDEXX Laboratories, Westbrook, ME, USA), and the following parameters were evaluated: red blood cells (RBCs), hemoglobin (Hb), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular Hb (MCH), MCH concentration (MCHC), red cell distribution weight (RDW), platelets, mean platelet volume (MPV), white blood cells (WBCs), neutrophil count (abs and %), lymphocyte count (abs and %), monocyte count (abs and %), eosinophil count (abs and %), and basophil count (abs and %). Serum chemistries were completed on a VetScan VS2 Chemistry Analyzer (Abaxis, Union City, CA, USA), and the following parameters were evaluated: glucose, blood urea nitrogen (BUN), creatinine, calcium, albumin, total protein, ALT, AST, ALP, total bilirubin, globulin, sodium, potassium, chloride, and total carbon dioxide. The complete blood hematology and chemistry data are provided in Table S2.
Hawman D.W., Meade-White K., Leventhal S., Appelberg S., Ahlén G., Nikouyan N., Clancy C., Smith B., Hanley P., Lovaglio J., Mirazimi A., Sällberg M, & Feldmann H. (2022). Accelerated DNA vaccine regimen provides protection against Crimean-Congo hemorrhagic fever virus challenge in a macaque model. Molecular Therapy, 31(2), 387-397.
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