Fine chemicals

The cDNA clones used in this study have been published elsewhere, Slc34a2a and Slc34a2a (as) by Nalbant et al. [40 (link)], Slc34a2b by Graham et al. [53 (link)], Sonic Hedgehog (Shh) by Danesin et al. [54 (link)] and Engrailed 2 (Eng2) was a kind gift from C. Houart. Fine chemicals were purchased from Sigma Aldrich.

Solvents, and fine chemicals were purchased from SigmaAldrich (Gillingham, Dorset, UK) except phosSTOP tablets were purchased from Roche (Welwyn, Hertfordshire, UK; stored at 4 °C). Chemicals for the growth medium were purchased from Penta (Chrudim, CZ).

Fine chemicals were purchased from SIGMA-Aldrich (St. Louis, MO), ATP and ADP were obtained from Roche Applied Science, Mannheim, Germany. Radicicol (RA) was obtained from IRIS Biotech GmbH, Marktredwitz, Germany. Deuterium oxide was purchased from Euriso-top, Gif-sur-Yvette, France. His-tagged Ulp1 was prepared in-house.

Fine chemicals and yeast synthetic drop-out medium supplements without uracil were
procured from Sigma. All other media components were obtained from BD (Difco).
Oligonucleotides were custom synthesised from Sigma-Genosys, India. Restriction
enzymes, DNA polymerases and other DNA modifying enzymes were obtained from New
England Biolabs, and DNA purification kits were obtained from Qiagen.

The chemicals, reagents and solvents used were of analytical grade and obtained from Sisco Research Laboratories (Mumbai, Maharashtra, India) and Merck (Whitehouse Station, NJ, USA). Fine chemicals, cell culture media, KiCQ start PCR primers and anti-β-actin antibody were obtained from Sigma-Aldrich (St. Louis, MO, USA). Fetal Bovine Serum was procured from PAN Biotech (GmbH, Germany). DNA extraction kit and cDNA conversion kit were procured from Qiagen (Venlo, Netherlands) and Applied Biosystems (Foster city, CA, USA) respectively. SyBR green was procured from Takara Bio (Kusatsu, Japan). Electrophoresis and Western blot reagents were from Bangalore Genie (Bangalore, Karnataka, India), Bio-Rad Laboratories (Hercules, CA, USA) and Millipore (Billerica, MA, USA). ATP: ADP assay kit was obtained from Abcam (Cambridge, MA, USA). All primary antibodies used for immunoblotting and immunocytochemistry (Supporting Table 1) were obtained from Abcam and Cell Signaling Technology (Danvers, MA, USA). HRP-conjugated secondary antibodies were obtained from Bangalore Genei. Reagent kits and other consumables for transcriptomics (cDNA microarray) were obtained from Agilent Technologies (Santa Clara, CA, USA). HPLC columns were obtained from Waters Corporation (Milford, MA, USA) and other consumables from Sigma-Aldrich and Sisco Research Laboratories.

Unless specified otherwise, cell culture reagents (consumables and media) were from Gibco/Thermo Fisher Scientific (Waltham, USA), and fine chemicals (inhibitors, substrates) were from Sigma-Aldrich (Steinheim, GER). CAS identifiers of the tested set of chemicals are compiled in Suppl. Fig. 1.