AuNRs were deposited on carbon-coated copper grids (Ted Pella) and air-dried prior to TEM (JEOL JEM-2100 TEM operated at 200 kV). Images were recorded on a Gatan 1.35 K × 1.04 K ×12 bit ES500W CCD camera.
Carbon coated copper grid
Manufactured by Ted Pella
Sourced in United States
Carbon-coated copper grids are lab equipment used as sample supports for transmission electron microscopy (TEM) imaging. They provide a stable and conductive surface for mounting specimens, enabling high-resolution imaging and analysis of microscopic samples.
Automatically generated - may contain errors
Lab products found in correlation
Uranyl acetate, by Zeiss (4 mentions)
Uranyl acetate, by Ted Pella (4 mentions)
Energy filter tem libra 120, by Zeiss (4 mentions)
Uranyl acetate, by Electron Microscopy Sciences (4 mentions)
Zetasizer nano z, by Malvern Panalytical (3 mentions)
Dts1070, by Malvern Panalytical (2 mentions)
Nanosight ns300, by Malvern Panalytical (2 mentions)
Ht7700 transmission electron microscope, by Hitachi (2 mentions)
H 7650, by Hitachi (2 mentions)
Jem 2100 tem, by JEOL (1 mentions)
Es500w ccd camera, by Ametek (1 mentions)
Nanosight system, by Malvern Panalytical (1 mentions)
Lambda 850 uv vis spectrophotometer, by PerkinElmer (1 mentions)
Jem 2100f, by JEOL (1 mentions)
Zetasizer, by Malvern Panalytical (1 mentions)
Ifs 66v s, by Bruker (1 mentions)
Jem 1100, by JEOL (1 mentions)
Nanoseries zetasizer zs 90, by Malvern Panalytical (1 mentions)
Tecnai f20, by Thermo Fisher Scientific (1 mentions)
Disposable polystyrene cuvette, by Sarstedt (1 mentions)
51 protocols using carbon coated copper grid
1
Characterizing AuNRs by Transmission Electron Microscopy
2
Characterization of PEG-coated Gold Nanoparticles
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The XRD pattern of the PEG-AuNP samples was recorded by a Jordan Valley’s D1 (Jordan Valley Semiconductors Ltd) equipped with a Cu Kα (λ = 1.5406 A°) radiation filter. The obtained PEG-AuNP XRD patterns were then compared with JCPDS cards (JCPDS # 04–0784) present in the XRD software. The chemical structure and functional groups of the PEG-AuNPs were analysed by Fourier transform infrared spectroscopy (FT-IR, Bruker Optics IFS 66 V/S, Germany). The spectrum was measured in transmission mode ranging from 4000–600 cm−1 wave numbers by KBr pellet method. UV–visible spectroscopy was performed with a double-beam Lambda 850 UV/VIS spectrophotometer (Perkin-Elmer Inc., USA). Conjugated and unconjugated samples were analysed in the 400–900 nm spectral range. All spectra were recorded using a 0.0125 mg/mL solution of the PEG-AuNPs. The assembly and morphology of PEG-AuNPs was studied with TEM (JEOL, JEM 2100F). TEM samples were prepared onto carbon-coated copper grids (Ted Pella, USA) and were dried at room temperature for microscopic observation. Electrophoretic mode of zetasizer (Malvern, UK) was used to measure the zeta potential (ζ) on PEG-AuNPs surface. The PEG-AuNPs hydrodynamic diameters were measured by using nanoparticle tracking analysis (NTA). NTA was carried out at room temperature by using a NanoSight system (Malvern, UK).
3
Negative Staining of Aβ Fibrils
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Aβ fibrils spread on carbon-coated copper grids (Ted Pella Inc., Redding, CA, USA) were negatively stained with 1% (w/v) uranyl acetate (Electron Microscopy Sciences, Hatfield, PA, USA) and examined with an HT7700 transmission electron microscope (HT7700, Hitachi High-Technologies, Tokyo, Japan) with an accelerating voltage of 100 kV. Images were acquired using a CCD (charge-coupled device) camera before being further processed in Adobe Photoshop CS6 (Adobe, San Jose, CA, USA).
4
Polyplexes Characterization via TEM
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Polyplexes (pDNA concentration 10 μg/mL) were formed in water instead of HBG. The preparation of carbon-coated copper grids (Ted Pella, USA, 300 mesh, 3.0-mm OD) and the staining procedure was performed as described previously.46 All grids were analyzed with a JEOL JEM-1100 (JEOL, Tokyo, Japan) electron microscope at 80 kV acceleration voltage.
5
Transmission Electron Microscopy of hUC-MSC-EVs
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Freshly isolated hUC-MSC-EVs in 150 μl of 1xPBS suspension were loaded onto carbon-coated copper grids (Ted Pella, USA) and incubated for 10 min. The grid was blotted with filter paper and stained with 2% uranyl acetate (Ted Pella, USA) for 1 min. Excessive uranyl acetate was removed, and the grid was let dry for 15 min before viewing using Energy Filter TEM Libra-120 (Carl Zeiss AG, Germany).
6
Characterizing PLGA-THPC Nanostar Morphology
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The morphology and size of PLGA-THPC nanostars were examined using a transmission electron microscope (TEM; Tecnai F20; FEI company, Hillsboro, OR, USA) at an accelerating voltage of 120 kV. A drop of 10 μL of a previously prepared PLGA-THPC nanostar suspension was pipetted onto carbon-coated copper grids (Ted Pella, Redding, CA, USA) and air-dried. The diameter of nanostars was measured using ImageJ (version 1.45S, NIH, Bethesda, MD, USA) for at least 20 particles. The size and surface charges of nanostars were measured by dynamic light scattering using a Nanoseries Zetasizer ZS 90 (Malvern Instruments Ltd., Malvern, Worcestershire, UK), with backscattering detection at 90°.
7
Characterization of Nanoparticle Formulations
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Particle sizing was carried out using dynamic light scattering with a Zetasizer Nano ZS (Malvern Instruments Ltd., Worcestershire, UK). For each measurement, the sample suspension without further treatment (~1.0 mL) was put in a disposable polystyrene cuvette (SARSTEDT AG & Co., Nümbrecht, Germany). The nanocarrier surface ζ-potential was measured using a Zetasizer Nano ZS by using disposable folded capillary cells (DTS1070, Malvern Instruments, Malvern, UK). Both the size and ζ-potential were recorded as the average of three measurements. The structure of the fabricated nanoparticle was evaluated by transmission electron microscopy (TEM; JEOL 1400 Plus, JEOL, Tokyo, Japan) at an acceleration voltage of 120 kV. The TEM samples were prepared by depositing 10 µL of the NP suspensions (~1.0 mg/mL) onto carbon-coated copper grids (200 mesh; Ted Pella, Inc., Redding, CA, USA). Samples were blotted away after 5 min incubation then air-dried prior to imaging.
8
Negative Staining of HCVpp for TEM
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Observation by TEM after negative staining with phosphotungstic acid (2% w/v, adjusted to pH 7.0 just before use) was performed on a JEOL JEM 1400 microscope operated at 80 kV and equipped with a high port digital camera Gatan Orius 600 (CIQLE facility, SFR Lyon Est). Carbon-coated copper grids were purchased from Ted Pella Inc. Prior to their use, grids were submitted to glow discharging, followed by immediate sample deposition. Labelled HCVpp retained their integrity, and displayed a similar morphology as that of unlabelled HCVpp, with neither particle aggregation nor lysis (Supplementary Fig. 4 ).
9
Transmission Electron Microscopy of Amyloid-beta Fibrils
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A carbon-coated copper grids (Ted Pella Inc., Redding, CA, USA) was put on a drop of sample containing Aβ25–35 for 5 min. Sample were then negatively stained with 1% (w/v) uranyl acetate (Electron Microscopy Sciences, Hatfield, PA, USA) before examination under a transmission electron microscope (HT7700, Hitachi High-Technologies, Tokyo, Japan) with an accelerating voltage of 100 kV. High-resolution images were acquired by a CCD (charge-coupled device) camera and processed further in the Adobe Photoshop CC (Adobe, San Jose, CA, USA).
10
Transmission Electron Microscopy of Phage Lysates
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High titer phage lysates (109 – 1011 PFU/ml) were chosen for transmission electron microscopy (TEM) and prepared for imaging as described previously (Deveau et al., 2006 (link)), with modifications. Briefly, one ml of lysate was spun at 4°C for 1.5 h at 21,000 × g. The supernatant was subsequently discarded and the last 100 μl was saved. Consequently, 1 ml of 0.1 M ammonium acetate (Amresco, Solon, OH, United States) was added and the suspension subsequently spun again at 4°C for 1.5 h at 21,000 × g. This purification was repeated twice, with the last 100 μl reserved for TEM.
For grid preparation, 3 μl of purified lysates were placed on carbon coated copper grids (Ted Pella, Redding, CA, United States) following glow-discharge. The phage preparations were subsequently negatively stained with 2% phosphotungstic acid (Ted Pella). A Hitachi H-7600 transmission electron microscope was used for acquiring the images at the University of British Columbia Bioimaging Facility. An accelerating voltage of 80 kV was used for imaging.
For grid preparation, 3 μl of purified lysates were placed on carbon coated copper grids (Ted Pella, Redding, CA, United States) following glow-discharge. The phage preparations were subsequently negatively stained with 2% phosphotungstic acid (Ted Pella). A Hitachi H-7600 transmission electron microscope was used for acquiring the images at the University of British Columbia Bioimaging Facility. An accelerating voltage of 80 kV was used for imaging.
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