Cell apoptosis and cell cycle analysis were examined on the Muse Cell Analyzer (EMD Millipore, Billerica, MA, USA). MDA-MB-231 cells were plated into 24-well plates at the density of 5 × 104 cells/well. Cells were incubated with MOL extract (150 µg/mL), fractions no. 1–11 (150 µg/mL), 7-octenoic acid (2.5 and 4 mg/mL), oleamide (70 and 100 μg/mL), 1-phenyl-2-pentanol (600 and 700 μg/mL), or doxorubicin (1.5 μM) for 24 h. Cell apoptosis was examined by staining with Muse™ Annexin V and Dead Cell reagent (EMD Millipore, Billerica, MA, USA; cat. no. MCH100105). For cell cycle analysis, cells were fixed in chilled 75% ethanol for 3 h at −20 °C. Then cells were washed with PBS and incubated with Muse™ Cell Cycle Reagent (Millipore, Billerica, MA, USA; cat. no. MCH100106) for 30 min in the dark at RT. Cells were read on the Muse cell analyser.
Muse cell cycle reagent
Manufactured by Merck Group
Sourced in United States, Germany
The Muse Cell Cycle Reagent is a laboratory product designed to assist in the analysis of cell cycle progression. It provides a standardized and consistent method for determining the distribution of cells within the different phases of the cell cycle.
Automatically generated - may contain errors
Lab products found in correlation
Muse cell analyzer, by Merck Group (27 mentions)
Macsquant analyzer, by Miltenyi Biotec (6 mentions)
Macsquantify software version 2, by Miltenyi Biotec (4 mentions)
Muse annexin 5 and dead cell reagent, by Merck Group (2 mentions)
Muse cell cycle assay kit, by Merck Group (2 mentions)
Flow cytometry, by Thermo Fisher Scientific (1 mentions)
Annexin 5 enhanced green fluorescent protein, by Thermo Fisher Scientific (1 mentions)
Propidium iodide, by Thermo Fisher Scientific (1 mentions)
Cytoflex flow cytometer, by Beckman Coulter (1 mentions)
Muse cell soft v1.4.0.0 analyzer assays, by Merck Group (1 mentions)
Muse analyzer, by Merck Group (1 mentions)
Muse analysis software, by Merck Group (1 mentions)
Macsquantify v2, by Miltenyi Biotec (1 mentions)
Muse 1.5 analysis software, by Merck Group (1 mentions)
Muse annexin 5, by Merck Group (1 mentions)
Muse cell analyzer system, by Merck Group (1 mentions)
Dead cell reagent, by Merck Group (1 mentions)
Macsquantify software, by Miltenyi Biotec (1 mentions)
Macsquant analyzer flow cytometer, by Miltenyi Biotec (1 mentions)
Propidium iodide, by BD (1 mentions)
50 protocols using muse cell cycle reagent
1
Apoptosis and Cell Cycle Analysis of MDA-MB-231 Cells
2
Cell Cycle Analysis by Flow Cytometry
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The cell cycle of cells in different groups was detected by Flow cytometry. Simply, cells were washed with PBS, fixed in 70% pre-cooled ethanol, and stained with Muse Cell Cycle Reagent (Millipore, USA). After 30 min of incubation under darkness, the percentage of cells in different cell cycle phases was analyzed on Flow cytometry (Invitrogen).
3
Cell Cycle Analysis of GMCs
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GMCs were fixed in 70% ethanol for 12 h at 4°C, and washed with phosphate buffer saline (PBS). After incubating with Muse Cell Cycle Reagent (Millipore, U.S.A.) in the dark for 30 min, cells were analyzed on MUSE cell analyzer (Millipore).
4
Cell Cycle Analysis of B16F10 Melanoma Cells
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B16F10 cells from each group were washed three times and resuspended in 50 µL PBS. Suspended cells were added into the tube containing 1 mL of ice cold 70% ethanol in a dropwise manner while vortexing at gentle speeds. The tubes were frozen at −20 °C for 3 h prior to staining. Subsequently, the cells were washed and treated with 200 µl of Muse® Cell Cycle reagent (Millipore) according to the manufacturer’s protocol. After 30 min of incubation at room temperature in the dark, cell suspension samples were transferred into 1.5 mL microcentrifuge tubes and analyzed using the Muse™ Cell Analyzer.
5
Erianin Induces Cell Cycle Arrest and Apoptosis in Liver Cancer
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The liver cancer cells were plated into 6-well plates at 2×105 cells/well and exposed to erianin at 40 and 80 nM for 12 h. After fixation in 70% ethanol at 4°C overnight, cells were stained with Muse™ Cell Cycle reagent (Millipore, Billerica, MA, USA) under darkness at room temperature for 30 min. Cell cycle progression was analyzed using the Muse® Cell Analyzer (Millipore, Billerica, MA, USA).
The liver cancer cells were plated into 6-well plates at 2×105 cells/well and exposed to erianin at 40 and 80 nM for 24 h. Cells were stained with Muse™ Annexin V and Dead Cell reagent (Millipore, Billerica, MA, USA) under darkness at room temperature for 20 min. Cell apoptosis was analyzed using the Muse® Cell Analyzer (Millipore, Billerica, MA, USA).
The liver cancer cells were plated into 6-well plates at 2×105 cells/well and exposed to erianin at 40 and 80 nM for 24 h. Cells were stained with Muse™ Annexin V and Dead Cell reagent (Millipore, Billerica, MA, USA) under darkness at room temperature for 20 min. Cell apoptosis was analyzed using the Muse® Cell Analyzer (Millipore, Billerica, MA, USA).
6
Cell Cycle Analysis of Sarcoma and Fibroblasts
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Sarcoma and fibroblast cells were treated with DMSO or 25 µmol/L AR‐A014418 for 24 hours. The cells were then trypsinized, washed twice with PBS and fixed with 70% ethanol for 3 hours at −20°C. The fixed cells (1 × 106) were labeled with Muse cell cycle reagent (Millipore) for 30 min at room temperature in the dark and then analyzed for cell cycle fractions by flow cytometry using Muse Cell Analyzer (Millipore). Expression of proteins involved in cell cycle regulation (cyclin D1, CDK4 and CDK6) was examined by western blotting.
7
Cell Cycle Analysis by Flow Cytometry
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A total of 1 × 106 cells were washed three times and resuspended in cold PBS. Resuspended cells were added into the tube containing 1 ml of ice cold 70% ethanol while vortexing at medium speed. The tubes were frozen at −20°C for 3–24 h prior to staining. Subsequently, cells were washed and treated with 200 μl of Muse™ Cell Cycle reagent (Millipore Corp.) according to the manufacturer's protocol. After 30 min of incubation at room temperature in the dark, cell suspension samples were transferred into 1.5‐ml microcentrifuge tubes and analyzed using the Muse™ Cell Analyzer (Millipore Corp.).
8
Cell Cycle Analysis by Flow Cytometry
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A total of 1×10e6 cells were collected and washed with PBS before being fixed in 70% ice-cold ethanol. After incubation for at least 3 hours at -20°C, the cells were washed again and incubated with 200 μl of Muse™ Cell Cycle Reagent (Millipore, Billerica, MA, USA. cat # MCH100106) containing propidium iodide (PI) for 30 min at room temperature in the dark. The percentages of cells in the G0/G1, S and G2/M phases were read on a Muse Cell Analyzer (Millipore, Billerica, MA, USA). Each experiment was performed in triplicate, and the data are expressed as the mean ± SD.
9
Cell Cycle and Apoptosis Analysis
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To assess the cell cycle status of the cells, transfected HEC-1A and HEC-1B cells were fixed in 70% pre-cooled ethanol and stained with Muse™ Cell Cycle Reagent (Millipore, USA) for 30 min in the dark. The number of cells in each phase of the cell cycle was detected with a MUSE Cell Analyzer (Millipore). To detect cell apoptosis, different groups of cells were sequentially stained with annexin V-Enhanced Green Fluorescent Protein (EGFP) and Propidium Iodide (PI) (Thermo Fisher Scientific, USA). After incubation for 15 min in the dark, the apoptotic rate was detected with a MUSE Cell Analyzer (Millipore).
10
Cell Cycle Analysis by Flow Cytometry
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Trypsinized cells were pelleted by centrifugation, fixed in 70% ice-cold ethanol overnight at −20°C, and incubated with Muse™ Cell Cycle reagent (Merck Milipore). DNA contents were analyzed using a MACSQuant Analyzer (Miltenyi Biotec GmbH, Germany) according to fluorescence of propidium iodide (PI).
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