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Fitc conjugated anti murine igm antibody

Manufactured by Thermo Fisher Scientific
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FITC-conjugated anti-murine IgM antibody is a detection reagent used to identify and quantify murine IgM antibodies in biological samples. The antibody is conjugated with the fluorescent dye FITC, which allows for the visualization and analysis of IgM-positive cells using flow cytometry or fluorescence microscopy.

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Lab products found in correlation

Recombinant murine il 4, by R&D Systems (2 mentions) Pe conjugated anti murine iga antibody clone 11 44 2, by Thermo Fisher Scientific (2 mentions) Cytofix cytoperm and perm wash buffer, by BD (2 mentions) Recombinant human tgf β1, by R&D Systems (2 mentions) Facscalibur, by BD (2 mentions)

Close spelling variants of this product

FITC-conjugated anti-IgM Anti-IgM-FITC Anti-IgM antibodies IgM-FITC

2 protocols using fitc conjugated anti murine igm antibody

1

Knockdown Effects on Class Switch Recombination

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Class switch recombination was performed in CH12F3-2a cells as described previously50 (link). Briefly, RNF8, RNF168, L3MBTL2 or a combination of these, was knocked down using shRNAs. 40 hours later, cells were stimulated with ligands [1 ng/ml of recombinant human TGF-β1 (R&D Systems), 10 ng/ml of recombinant murine IL-4 (R&D Systems), and 250 ng/ml recombinant murine CD40 ligand (PerproTech)]. For analyzing class switch from IgM (IgM+/IgA−) to IgA (IgM−/IgA+), CH12F3-2 cells were collected after 60 hours, intracellularly stained with PE-conjugated anti-murine IgA antibody (clone 11–44-2, eBiosciences, Cat# 12-5994-82). Membrane IgM expression was assessed using FITC-conjugated anti-murine IgM antibody (eBiosciences; Cat# 11-5890-82). Cytofix/Cytoperm and Perm/Wash buffers (BD Biosciences, catalog # 554714) was utilized. Cells were then analyzed on a FACS Calibur (BD Biosciences) at the Mayo Clinic Flow Cytometry Core. Data was analyzed using FlowJo software (TreeStar).
Nowsheen S., Aziz K., Aziz A., Deng M., Qin B., Luo K., Jeganathan K.B., Zhang H., Liu T., Yu J., Deng Y., Yuan J., Ding W., van Deursen J.M, & Lou Z. (2018). L3MBTL2 orchestrates ubiquitin signaling by dictating the sequential recruitment of RNF8 and RNF168 after DNA damage. Nature cell biology, 20(4), 455-464.
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2

Knockdown Effects on Class Switch Recombination

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Class switch recombination was performed in CH12F3-2a cells as described previously50 (link). Briefly, RNF8, RNF168, L3MBTL2 or a combination of these, was knocked down using shRNAs. 40 hours later, cells were stimulated with ligands [1 ng/ml of recombinant human TGF-β1 (R&D Systems), 10 ng/ml of recombinant murine IL-4 (R&D Systems), and 250 ng/ml recombinant murine CD40 ligand (PerproTech)]. For analyzing class switch from IgM (IgM+/IgA−) to IgA (IgM−/IgA+), CH12F3-2 cells were collected after 60 hours, intracellularly stained with PE-conjugated anti-murine IgA antibody (clone 11–44-2, eBiosciences, Cat# 12-5994-82). Membrane IgM expression was assessed using FITC-conjugated anti-murine IgM antibody (eBiosciences; Cat# 11-5890-82). Cytofix/Cytoperm and Perm/Wash buffers (BD Biosciences, catalog # 554714) was utilized. Cells were then analyzed on a FACS Calibur (BD Biosciences) at the Mayo Clinic Flow Cytometry Core. Data was analyzed using FlowJo software (TreeStar).
Nowsheen S., Aziz K., Aziz A., Deng M., Qin B., Luo K., Jeganathan K.B., Zhang H., Liu T., Yu J., Deng Y., Yuan J., Ding W., van Deursen J.M, & Lou Z. (2018). L3MBTL2 orchestrates ubiquitin signaling by dictating the sequential recruitment of RNF8 and RNF168 after DNA damage. Nature cell biology, 20(4), 455-464.
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