Rabbit igg hrp g 21234

Gambogic acid (GA) was purchased from Biorbyt (San Francisco, CA, UK). Manganese (III) tetrakis (4-benzoic acid) porphyrin chloride (MnTBAP) was from Calbiochem (EMD Millipore Corp., Billerica, MA, USA). MitoTracker-Red (MTR), propidium iodide (PI), 5-(and-6)-chloromethyl-2′,7′-dichlorofluorescein diacetate (CM-H₂DCF-DA), and tetramethylrhodamine methyl ester (TMRM) along with secondary antibodies (rabbit IgG HRP (G-21234) and mouse IgG HRP (G-21040)) were from Molecular Probes (Eugene, OR, USA). The primary antibody against SDHA (ab14715) was from Abcam (Cambridge, MA, USA) and Noxa (OP180) was from Calbiochem. β-actin (sc-47778), ubiquitin (sc-8017), Mcl-1 (sc-819), ATF4 (sc-200) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Phospho-SAPK/JNK (#9251), SAPK/JNK (#9252), phospho-ERK1/2 (#9101), ERK1/2 (#9102), TCF11/NRF1 (#8052), CHOP/GADD153 (#2895), phospho-eIF2α (#9721), and eIF2α (#9722) were from Cell Signaling Technology (Danvers, MA, USA). PDI (ADI-SPA-890-F) was form Enzo Life Science (Farmingdale, NY, USA). Other reagents were from Sigma-Aldrich (St Louis, MO, USA).

Lercanidipine, Amlodipine, Nicardipine, Niguldipine, and Felodipine were purchased from Tocris (Tocris Bioscience, Bristol, UK). Bortezomib and Carfilzomib were from Selleckchem (Houston, TX, USA). Ixazomib (MLN9708) was purchased from Cayman Chemical (Ann Arbor, MI, USA). Necrostatin-1, 3-methyladenine (3-MA), bafilomycin A1, 2-bis(o-aminophenoxy)ethane-N, N, N’N’tetraacetic acid acetoxymethyl ester (BAPTA-AM), cycloheximide and ruthenium red (RR) were from Sigma-Aldrich (St Louis, MO, USA). Ru360 was from Calbiochem (EDM Millipore Corp., Billerica, MA, USA). MitoTracker-Red (MTR), propidium iodide (PI), Fluo-3-AM, Rhod-2-AM and tetramethylrhodamine methyl ester (TMRM) were purchased from Molecular Probes (Eugene, OR, USA). z-VAD-fmk was from R&D systems (Minneapolis, MN, USA). The primary antibody against ubiquitin (sc-8017), ATF4 (sc-200) and β-actin (sc-47778) and were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). p62 (610833) was purchased from BD sciences (San Jose, CA, USA). Cleaved caspase-3 (#9661), LC3B (#2775), PERK (#5683), p-eIF2α (#9721), eIF2α (#9722), and CHOP/GADD153 (#2895) were purchased from Cell Signaling Technology (Danvers, MA, USA). Secondary antibodies (rabbit IgG HRP(G-21234) and mouse IgG HRP (G-21040)) were from Molecular Probes.

Chemicals and reagents were obtained as follows: bortezomib (Bz) and carfilzomib (Cfz) from Selleckchem (Houston, TX, USA); Necrostatin-1, bafilomycin A1, N-acetyl-L-cysteine (NAC), and H₂O₂ from Sigma-Aldrich (St Louis, MO, USA); cycloheximide (CHX), SP600125, PD98059, and Manganese (III) tetrakis (4-benzoic acid) porphyrin chloride (MnTBAP) from Calbiochem (EDM Millipore Corp., Billerica, MA, USA); Auranofin (AF) from AdipoGen (Liestal, CH, Switzerland); MitoTracker-Red (MTR), propidium iodide, and 5-(and-6)-chloromethyl-2′,7′-dichlorofluorescein diacetate (CM-H₂DCF-DA) from Molecular Probes (Eugene, OR, USA); z-VAD-fmk from R&D systems (Minneapolis, MN, USA); Glutathione reduced ethyl ester (GSH-OEt) from Chemodex (Gallen, CH, Switzerland); SB203580 from Tokyo Chemical Industry (TCI, Tokyo, Japan); CDDO-ME from Cayman (Michigan, USA). The following primary antibodies were used: TrxR1 (HPA001395) from Sigma-Aldrich; Ubiquitin (sc-8017) and β-actin (sc-47778) from Santa Cruz Biotechnology (Santa Cruz, CA, USA); ATF4 (#11815), p-eIF2α (#9721), eIF2α (#9722), and CHOP/GADD153 (#2895) from Cell Signaling Technology (Danvers, MA, USA); CHAC1 (15207-1-AP) from Proteintech (Rosemont, IL, USA). The secondary antibodies (rabbit IgG HRP(G-21234) and mouse IgG HRP (G-21040)) were from Molecular Probes.