Cd3ε pe

Manufactured by BD

CD3ε-PE is a fluorescently labeled antibody that binds to the CD3 epsilon chain, a component of the T cell receptor complex. It is commonly used in flow cytometry applications to identify and quantify T cells in biological samples.

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Lab products found in correlation

Facscanto, by BD (1 mentions) Cd44 pe, by BD (1 mentions) Cd25 fitc, by BD (1 mentions) Cd45 apc, by BD (1 mentions) B220 apc cy7, by BD (1 mentions) Ki67 fitc, by BD (1 mentions) Cd2 fitc, by BD (1 mentions) Facscanto 2, by BD (1 mentions) B220 apc, by BD (1 mentions) Igm fitc, by BD (1 mentions)

Spelling variants of this product

PE-anti-CD3ε (4 citations) Anti–CD3ε-PE-Cy7 (2 citations)

2 protocols using cd3ε pe

Multiparameter Flow Cytometry Analysis

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Cells from the bone marrow, thymus, spleen, liver, and peripheral blood were stained and analyzed on a FACSCanto (BD Biosciences) running FACSDiva (v.6.1.3) and FlowJo (v.7.6.1, FlowJo LLC) software. Lymphocytes were identified and gated based on their forward-scattered light (FSC) and side-scattered light (SSC) profiles. Cell suspensions were treated with ACK lysis buffer (150 mmol/L NH₄Cl, 10 mmol/L KHCO₃, and 0.1 mmol/L disodium EDTA) and then stained with the following conjugated antibodies: B220-APC-Cy7 (BD Biosciences), CD2-FITC (BD Biosciences), CD3ε-PE (BD Biosciences), CD3ε-Alexaflore 647 (BD Biosciences), CD4-FITC (Caltag Laboratories), CD8a-APC (Caltag Laboratories), CD8a-Alexaflore 647 (BD Biosciences), CD25-FITC (BD Biosciences), CD44-PE (BD Biosciences), CD45-APC (BD Biosciences), Ki-67-FITC (BD Biosciences), Ly5.1-PerCP-Cy5.5 (BD Biosciences), Ly5.2-PerCP-Cy5.5 (BD Biosciences), NK1.1-APC (Caltag Laboratories), and Sca1-Alexaflore 647 (BD Biosciences).

Ginn S.L., Hallwirth C.V., Liao S.H., Teber E.T., Arthur J.W., Wu J., Lee H.C., Tay S.S., Hu M., Reddel R.R., McCormack M.P., Thrasher A.J., Cavazzana M., Alexander S.I, & Alexander I.E. (2016). Limiting Thymic Precursor Supply Increases the Risk of Lymphoid Malignancy in Murine X-Linked Severe Combined Immunodeficiency. Molecular Therapy. Nucleic Acids, 6, 1-14.

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Flow Cytometry Cell Analysis

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For flow cytometry, isolated cells were stained with the indicated antibodies and analyzed with a FACS-CantoII flow cytometer (BD Biosciences). IgM-FITC, CD3ε-PE, and B220-APC antibodies were purchased from BD Biosciences. Cell cycle analysis was performed as described.^{33 (link)} All results were analyzed with FlowJo software (FlowJo, LLC).

Ikeda M., Hayes C.K., Schaller S.J, & Longnecker R. (2022). Latent membrane proteins from EBV differentially target cellular pathways to accelerate MYC-induced lymphomagenesis. Blood Advances, 6(14), 4283-4296.

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