Macrocap sp

Momordica charantia L. seeds were provided by the Institute of Agricultural Science and Technique of Sichuan Province, China. Matrices for SDS-PAGE and IEF were products of Bio-Rad Laboratories (Hercules, CA, USA). The chromatography medium for MacroCap SP was purchased from GE Healthcare Bio-Sciences AB (Uppsala, Sweden). 2-(N-Morpholino)ethanesulfonic acid (MES) and agarose were the products of Sigma-Aldrich (St Louis, MO, USA). mPEG-SC (10 kDa) was obtained from Shearwater Polymers (Huntsville, AL, USA). Human lung adenocarcinoma epithelial A549 cells were provided by the West China Center of Medical Sciences of Sichuan University (Chengdu, China). All other chemical reagents were standard commercial products of analytical grade.

Momordica charantia L. seeds were obtained from the Institute of Agricultural Science and Technique of Sichuan Province, China. Electrophoresis reagents were products of Sigma-Aldrich (St Louis, MO). Macro-Cap-SP, SP-Sepharose FF, Sephacryl S-100HR and ampholyte were purchased from GE Healthcare Bio-Sciences AB (Uppsala, SE). Dulbecco's Modified Eagle's Medium (DMEM) and fetal bovine serum (FBS) used in cell culture were from Gibco BRL (Grand Island, NE). The lung adenocarcinoma A549 cell line was obtained from American Type Culture Collection (ATCC CCL-185) (Manassas, VA). LMW Calibration Kit was supplied by SIBAS (Shanghai, China). pUC18 DNA used in detection of topological activity was obtained from TAKARA (Dalian, China). All other chemical reagents were standard commercial products of analytical grade.