For pollen viability estimation through outer structure analysis, fresh pollen was observed with a Hitachi S-3000N variable pressure scanning electron microscope at 30 Pa and 15kV. For transmission electron microscopy (TEM), samples were fixed in 2.5% glutaraldehyde in 0.1M sodium cacodylate buffer pH 7.4, post-fixed in 2% osmium tetroxide (OsO4) in the same buffer, dehydrated in ethanol, and embedded in Spurr’s resin. Observations were made on a Hitachi H-7500 microscope. Statistics for pollen defects observed by scanning electron microscopy (SEM) were scored from ≥100 pollen grains per wild-type (WT) or transgenic line.
H 7500 microscope
Manufactured by Hitachi
Sourced in United States
The Hitachi H-7500 is a transmission electron microscope designed for high-resolution imaging and analysis of samples. It features a 100 kV accelerating voltage and advanced optics to provide detailed information about the structure and composition of materials at the nanoscale level.
Automatically generated - may contain errors
Lab products found in correlation
Eponate 12, by Ted Pella (2 mentions)
Ls 55 fluorescence spectrometer, by PerkinElmer (1 mentions)
Nano z, by Malvern Panalytical (1 mentions)
Fx pro imaging system, by Bruker (1 mentions)
Em uc7, by Leica (1 mentions)
Liquid releasing agent, by Electron Microscopy Sciences (1 mentions)
Nano zs laser particle size analyzer, by Malvern Panalytical (1 mentions)
K alpha x ray photoelectron spectrometer, by Thermo Fisher Scientific (1 mentions)
10 protocols using h 7500 microscope
1
Pollen Viability Estimation via SEM
2
Multidisciplinary Characterization of Nanomaterials
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Transmission electron microscopy (TEM) was carried out with a Hitachi H-7500 microscope operated at 80 kV. UV-visible (UV-vis) absorption spectra were performed using a Hewlett-Packard 8452 diode array spectrometer (U-3010). Particle size distribution and zeta potential were recorded by Malvern Nano-ZS (UK). Agarose gel electrophoresis (AGE) samples were acquired by using Powersupplies PowerPac HV (Bio-Rad, American) performed at 80 V for 50 min. Fluorescence spectra were measured by LS 55 Fluorescence Spectrometer (PerkinElmer, American). In vivo fluorescence imaging was scanned with Bruker FX PRO imaging system (Rochester, NY, USA).
3
Ultrastructural Analysis of Kidney Tissues
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Kidney tissues were fixed in 4% glutaraldehyde with 0.1M cacodylate buffer (pH 7.2) for 4 h. Samples were further rinsed in cacodylate buffer and post-fixed in 1% OsO4 with 0.1M cacodylate buffer (pH 7.4) for 24 h, dehydrated in a series of ethanol and infiltrated overnight in a 1:1 mixture of epoxy resin and propylene oxide. Specimens were embedded with Eponate 12 (Ted Pella, United States) and polymerized at 60°C for 24 h. Ultrathin sections were stained with lead citrate and uranyl acetate and viewed on a HITACHI H-7500 microscope.
4
Morphology and Pore Analysis of Porous Carbons
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The morphology
and pore size of the resultant porous carbons were examined on a field
emission scanning electron microscope (FE-SEM, JEOL JSM7000F, USA).
TEM was conducted using a Hitachi H-7500 microscope. The inorganic
residue in the resultant porous carbons and the carbon-to-inorganic
template ratio of the particle composition was characterized using
a TGA (TA Instruments Q50, USA), heated from 100 to 900 °C with
a ramp rate of 20 °C min–1 under an air or
nitrogen atmosphere. A micro Raman spectrometer from Renishaw with
a He–Ne laser source with a wavelength of 633 nm was used to
determine the structure of the porous carbons. The N2 adsorption–desorption
isotherms of all samples were taken at 77 K on a micrometric TriStar
II apparatus to estimate the pore sizes and surface areas. The pore-size
distribution curves were obtained from the analysis of adsorption
isotherms using the BJH (Barrett–Joyner–Halenda) method.
and pore size of the resultant porous carbons were examined on a field
emission scanning electron microscope (FE-SEM, JEOL JSM7000F, USA).
TEM was conducted using a Hitachi H-7500 microscope. The inorganic
residue in the resultant porous carbons and the carbon-to-inorganic
template ratio of the particle composition was characterized using
a TGA (TA Instruments Q50, USA), heated from 100 to 900 °C with
a ramp rate of 20 °C min–1 under an air or
nitrogen atmosphere. A micro Raman spectrometer from Renishaw with
a He–Ne laser source with a wavelength of 633 nm was used to
determine the structure of the porous carbons. The N2 adsorption–desorption
isotherms of all samples were taken at 77 K on a micrometric TriStar
II apparatus to estimate the pore sizes and surface areas. The pore-size
distribution curves were obtained from the analysis of adsorption
isotherms using the BJH (Barrett–Joyner–Halenda) method.
5
Ultrastructural Analysis of Rat Lung Tissue
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To examine ultrastructural changes in lung tissues, fresh lungs of the rats in 30-day group were cut into small pieces, fixed with 2.5% glutaraldehyde in 0.1 M phosphate buffer, post-fixed with 1% osmium tetroxide, dehydrated, and resin-embedded using an embedding apparatus (ZB-J0010; Zhongxing Bairui, Beijing, People’s Republic of China). Ultrathin (60 nm) sections of tissue were cut using an ultra-microtome (EM UC7; Leica, Wetzlar, Germany), collected on grids, and observed by TEM using a H7500 microscope (Hitachi) operated at 80 kV. Images were acquired with a charge-coupled device camera.
6
Ultrastructural Analysis of EYFP-Labeled Boutons
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Glutaraldehyde (0.1%) was added to the fixative for the rats used for the electron microscopic (EM) observation of EYFP‐labeled boutons, and the brains were cut into 50 μm thick horizontal sections on a Vibratome. The sections containing STN were equilibrated in 30% sucrose, freeze‐thawed in liquid nitrogen, and then processed similarly to those described for light microscopy, with the exception that all the procedures were performed at 4 °C, and the sections were not treated with Triton X‐100. After diaminobenzidine reaction, sections were postfixed with 1% osmium tetroxide, stained en bloc in 1.5% aqueous uranyl acetate, infiltrated with Epon‐Araldite resin, mounted on Liquid Releasing Agent (Electron Microscopy Sciences, 70880) coated glass slides, and coverslipped. Areas of STN containing EYFP‐labeled boutons were remounted on plastic blocks, and ultrathin (55–60 nm) sections were cut. The thin sections were examined and photomicrographed at 60 KV on a JEOL 1200 microscope and at 100 KV on a Hitachi H7500 microscope.
7
Transmission Electron Microscopy of Mouse Kidney
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Kidney tissues of mice were fixed in 4% glutaraldehyde with 0.1 M cacodylate buffer (pH 7.2) for 4 h. Samples were further rinsed in cacodylate buffer and post-fixed in 1% OsO4 with 0.1 M cacodylate buffer (pH 7.4) for 24 h, dehydrated in a series of ethanol and infiltrated overnight in a 1:1 mixture of epoxy resin and propylene oxide. Specimens were embedded with Eponate 12 (Ted Pella, United States) and polymerized at 60°C for 24 h. Ultrathin sections were stained with lead citrate and uranyl acetate and viewed on a HITACHI H-7500 microscope.
8
Transmission Electron Microscopy of Ly-MgO NPs
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Transmission electron
microscopic analysis was performed to determine
the size and morphology of Ly-MgO NPs using a Hitachi H-7500 microscope.
For TEM studies, the carbon-coated 200 mesh copper grid was dipped
into a solution of MgO NPs dispersed in DDW.
microscopic analysis was performed to determine
the size and morphology of Ly-MgO NPs using a Hitachi H-7500 microscope.
For TEM studies, the carbon-coated 200 mesh copper grid was dipped
into a solution of MgO NPs dispersed in DDW.
9
Characterization of Au Nanoparticles and Arrays
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TEM was carried out with a Hitachi H–7500 microscope operated at 80 kV. UV-vis absorption spectra were performed using on a Hewlett–Packard 8452 diode array spectrometer (U–3010). XPS spectra were obtained by an ESCALAB 250 X-ray photoelectron spectrometer (Axis Ultra DLD, UK). Particle size distribution was recorded by Malvern Nano-ZS Laser Particle Size Analyzer (UK). SERS spectra were acquired using a confocal microprobe Raman system (LabRAM Aramis, France) operated with a He–Ne laser (632.8 nm) and the data acquisition times were 10 and 20 s for the Au NAs and isolated NPs, respectively. The SERS samples were prepared by adding 3.8 μL of 10–4 M R6G into 15 μL of 4.9 × 10–9 M isolated NPs and 111, 212 and 222 Au NAs, respectively, and then the mixed solutions were dropped and dried on glass for SERS measurements.
10
Raman, TEM, and XPS Characterization
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Raman analysis was performed using Raman spectra Renishaw inVia micro-Raman equipped with 514 nm laser and a Leica microscope. All spectra were recorded with 5% laser power using ×50 objective lens. The samples for Raman analysis were prepared by drop-casting 10 μL of the respective samples on Si window (ThorLabs) and dried for 24 h at room temperature.
TEM images were performed with Hitachi H7500 microscope (Tokyo, Japan) with an accelerating voltage of 80 kV, equipped with an AMT Hamamatsu camera (Tokyo, Japan). X-ray Photoelectron Spectroscopy (XPS) analyses were performed on a Thermo Scientific K-Alpha X-ray photoelectron spectrometer with a basic chamber pressure of 10 -8 -10 -9 bar with an anode using Al Kα radiation (hν = 1486.6 eV). The samples were analyzed as powders. A spot size of 400 μm was used. The survey spectra are the average of 10 scans with a pass energy of 200.00 eV and a step size of 1 eV.
TEM images were performed with Hitachi H7500 microscope (Tokyo, Japan) with an accelerating voltage of 80 kV, equipped with an AMT Hamamatsu camera (Tokyo, Japan). X-ray Photoelectron Spectroscopy (XPS) analyses were performed on a Thermo Scientific K-Alpha X-ray photoelectron spectrometer with a basic chamber pressure of 10 -8 -10 -9 bar with an anode using Al Kα radiation (hν = 1486.6 eV). The samples were analyzed as powders. A spot size of 400 μm was used. The survey spectra are the average of 10 scans with a pass energy of 200.00 eV and a step size of 1 eV.
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