Creatinine assay kit

Manufactured by Nanjing Jiancheng

Sourced in China, United Kingdom

The Creatinine Assay Kit is a laboratory tool used to measure the concentration of creatinine in various biological samples, such as serum, plasma, or urine. Creatinine is a byproduct of muscle metabolism and is filtered by the kidneys, making it an important marker for evaluating kidney function. The kit provides the necessary reagents and protocols to perform a quantitative analysis of creatinine levels.

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77 protocols using creatinine assay kit

Effects of Klotho on Uremic Inflammation

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THP-1 cells were incubated in the presence of PMA (160 nM) for 48 h. After that, the cells were incubated in PMA-free medium for 24 h, and then transfected with the Klotho expression plasmid in the presence or absence of 2 mM IS for 24 h. Cell culture supernatants were then collected and the levels of IL-10, TNFα, IL-6, and IL-1β cytokines quantified by ELISA (ExCellBIO, Shanghai China) according to the manufacturer’s protocol. In addition, the levels of BUN and CR in serum and urine of mice were analyzed using Urea and Creatinine Assay Kits, respectively (Nanjing Jiancheng Bioengineering Institute, Jiangsu, China), according to the manufacturer’s protocols. Furthermore, the level of B-type natriuretic peptide (BNP) in serum of mice was analyzed using the brain natriuretic peptide assay kit according to the manufacturer’s protocol.

Lv J., Chen J., Wang M, & Yan F. (2020). Klotho alleviates indoxyl sulfate-induced heart failure and kidney damage by promoting M2 macrophage polarization. Aging (Albany NY), 12(10), 9139-9150.

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Measuring BUN and Serum Creatinine

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The blood urea nitrogen (BUN) levels and serum creatinine (Scr) levels were measured using urea nitrogen test kits and creatinine assay kits (Jiancheng Bioengineering Institute, Nanjing, China). The experimental procedures outlined in the instruction manual were followed to determine the blood urea nitrogen and creatinine content.

Yu D., Huang W., Sheng M., Zhang S., Pan H., Ren F., Luo L., Zhou J., Huang D, & Tang L. (2024). Angiotensin-(1-7) Modulates the Warburg Effect to Alleviate Inflammation in LPS-Induced Macrophages and Septic Mice. Journal of Inflammation Research, 17, 469-485.

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Serum Biomarker Quantification Protocol

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Serum creatinine and urea nitrogen were detected using creatinine assay kits (No: C011-2-1 and No: C013-2-1) purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Urinary protein levels were assayed with the Quick Start Bradford Protein Assay kit (1-800-424-6723) from Bio-Rad (Hercules, California, USA).

Chen X.C., Wu D., Wu H.L., Li H.Y., Yang C., Su H.Y., Liu Z.J., Huang X.R., Lu X., Huang L.F., Zhu S.P., Pan Q.J., An N, & Liu H.F. (2022). Metformin improves renal injury of MRL/lpr lupus-prone mice via the AMPK/STAT3 pathway. Lupus Science & Medicine, 9(1), e000611.

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Serum Creatinine and Urea Analysis

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Blood from the heart was collected into homemade anticoagulant tubes with 3.8% sodium citrate, and serum was isolated by centrifugation at 3,000 × g for 10 min at 4°C. Serum creatinine (SCr) and blood urea nitrogen (BUN) were measured using creatinine assay kits and urea assay kits in accordance with the manufacturer's instructions (Nanjing Jiancheng Bioengineering Institute).

Jiang G., Xin R., Yuan W., Zhang L., Meng X., Sun W., Han H., Hou Y., Wang L, & Du P. (2020). Ligustrazine ameliorates acute kidney injury through downregulation of NOD2-mediated inflammation. International Journal of Molecular Medicine, 45(3), 731-742.

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Urine Analysis in Rat Metabolic Cages

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On Day 0 (for identifying the model establishment) or Day 28 (for evaluating the effects of Huashi Pill), the rats were fed in metabolic-cages and the 24-hour urine was harvested by incubating with 0.02% sodium azide (SinoPharm Co., Ltd., Shanghai, China) to prevent the appearance of bacteria. The urinary pH and urinary volume were evaluated and then the urine was used for the other urine biochemical analysis. The urine protein (urine protein quantitative kit, Cat. No. C035-2, Nanjing Jiancheng Bioengineering Inst., Nanjing, China), uric acid (uric acid assay kit, Cat. No. C012-1, Nanjing Jiancheng Bioengineering Inst.), calcium (calcium assay kit, Cat. No. C004-3, Nanjing Jiancheng Bioengineering Inst.), magnesium (magnesium assay kit, Cat. No. C005, Nanjing Jiancheng Bioengineering Inst.), phosphorus (phosphate assay kit, Cat. No. C006, Nanjing Jiancheng Bioengineering Inst.) were examined by utilizing the commercial kit on the semiautomatic photometer (Mode: 750, Hitachi, Tokyo, Japan) following to the instructions of the manufacturers.
Moreover, the levels of serum urea nitrogen (BUN) and creatinine (Cr) were also evaluated using the urea assay kit (Cat. No. C013-1, Nanjing Jiancheng Bioengineering Inst.) and creatinine assay kit (Cat. No. C011-2, Nanjing Jiancheng Bioengineering Inst.), according to the manufacturer’s instructions.

Yang A., Guo H., Fu M, & Liu M. (2019). Inhibitive Effects of Huashi Pill on Formation of Renal Stones by Modulating Urine Biochemical Indexes and Osteopontin in Renal Stone Rat Models. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 25, 8335-8344.

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Renal Function Evaluation Protocol

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To evaluate renal function, serum BUN and Cre concentrations were detected by the colorimetric and the creatinine assay kit (Nanjing Jiancheng, China), according to standard methods, respectively. NGAL concentration was measured by using the human lipocalin-2/NGAL ELISA kit (Boster Biological Technology Ltd., Wuhan, China) according to standard methods.

Qiongyue Z., Xin Y., Meng P., Sulin M., Yanlin W., Xinyi L, & Xuemin S. (2022). Post-treatment With Irisin Attenuates Acute Kidney Injury in Sepsis Mice Through Anti-Ferroptosis via the SIRT1/Nrf2 Pathway. Frontiers in Pharmacology, 13, 857067.

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Measurement of Urinary Phthalate Metabolites

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Seven urinary phthalate metabolites including MMP, MEP, MBP, monobenzyl phthalate (MBzP), MEHP, MEOHP, and mono (2-ethyl-5-hydroxyhexyl) phthalate (MEHHP) were measured through high-performance liquid chromatography-tandem mass spectrometry (6410LC-MS, Agilent Technologies Co., Santa Clara, CA, USA), using a previously reported methodology with modifications^{58 (link), 59 (link)}. Concentrations below the limit of detection (LOD) were assigned with LOD/√2 for calculations. Urine creatinine was measured using a creatinine assay kit based on the picric kinetic method (Jiancheng Bioengineering Institute, Nanjing, China).

Gao H., Zhang Y.W., Huang K., Yan S.Q., Mao L.J., Ge X., Xu Y.Q., Xu Y.Y., Sheng J., Jin Z.X., Zhu P., Tao X.G., Hao J.H, & Tao F.B. (2017). Urinary concentrations of phthalate metabolites in early pregnancy associated with clinical pregnancy loss in Chinese women. Scientific Reports, 7, 6800.

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Evaluating blood creatinine and BUN

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The blood creatinine and BUN levels were evaluated using a creatinine assay kit and BUN assay kit, respectively, according to the manufacturer's instructions (Jiancheng Bioengineering Institute, Nanjing, China).

Wang S., Tian X., Li Y., Xue R., Guan H., Lu M., Xu H., Ye Z., Chen S, & Xiang M. (2020). Intracellular Reactive Oxygen Species Mediate the Therapeutic Effect of Induced Pluripotent Stem Cells for Acute Kidney Injury. Oxidative Medicine and Cellular Longevity, 2020, 1609638.

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Serum Creatinine and ALT Assay

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The serum creatinine (Scr) and alanine aminotransferase (ALT) levels were determined using a Creatinine Assay Kit and an Alanine Aminotransferase Assay Kit (Nanjing Jiancheng Bioengineering Institute, China).

Yang K., Fan B., Zhao Q., Ji Y., Liu P., Gao S., Ren T., Dou Y., Pei M, & Yang H. (2020). Hirudin Ameliorates Renal Interstitial Fibrosis via Regulating TGF-β1/Smad and NF-κB Signaling in UUO Rat Model. Evidence-based Complementary and Alternative Medicine : eCAM, 2020, 7291075.

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Quantifying Murine Microalbuminuria

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Twenty-four-hour urine samples were collected one day before the sacrifice of mice. Microalbumin and urinary creatinine level were measured with Albumin Mouse ELISA Kit (Abcam, Cambridge, UK) and Creatinine Assay Kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China), according to the manufacturer's protocol. Results of mouse microalbuminuria were expressed as protein/urine creatinine (in μg/mg).

Wang J.N., Yang Q., Yang C., Cai Y.T., Xing T., Gao L., Wang F., Chen X., Liu X.Q., He X.Y., Wei B., Jiang L., Li C., Jin J., Wen J.G., Ma T.T., Chen H.Y., Li J, & Meng X.M. (2020). Smad3 promotes AKI sensitivity in diabetic mice via interaction with p53 and induction of NOX4-dependent ROS production. Redox Biology, 32, 101479.

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