Goat anti mouse gpnmb

Manufactured by R&D Systems

Sourced in United States

Goat anti-mouse Gpnmb is a laboratory reagent used for the detection and analysis of the Gpnmb (Glycoprotein nmb) protein in mouse samples. This product is an antibody raised in goats against the mouse Gpnmb protein, which can be used in various immunoassay techniques to identify and quantify the target protein.

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Lab products found in correlation

Oct compound, by Electron Microscopy Sciences (1 mentions) Rat anti mouse lamp1, by BD (1 mentions) Dmem f12, by Corning (1 mentions) Goat anti olig2, by R&D Systems (1 mentions) Mouse anti galectin 3, by BioLegend (1 mentions) Goat anti human gpnmb, by R&D Systems (1 mentions) Goat anti aif 1 iba1, by Novus Biologicals (1 mentions) Sheep anti pgrn, by R&D Systems (1 mentions) Mouse anti mbp, by Merck Group (1 mentions) Mouse anti apc, by Merck Group (1 mentions) Sheep anti trem2, by R&D Systems (1 mentions) Odyssey blocking buffer, by LI COR (1 mentions) Dulbecco modified eagle medium (dmem), by Corning (1 mentions) Rabbit anti iba1, by Fujifilm (1 mentions) Trypsin, by Corning (1 mentions) Rat anti cd68, by Bio-Rad (1 mentions) Rat anti mouse f4 80, by Bio-Rad (1 mentions) Rat anti mouse cd68, by Bio-Rad (1 mentions) Monoclonal antibody against α sma, by Merck Group (1 mentions)

2 protocols using goat anti mouse gpnmb

Quantitative Histological and Biochemical Assessment of Liver Injury

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Hematoxylin-eosin and Sirius red staining were performed according to standard protocols. For immunohistochemical analysis, liver specimens were fixed in 10% buffered formalin or 4% paraformaldehyde and incubated with rat anti-mouse F4/80 (Clone: A3-1, AbD Serotec, Raleigh, NC, USA), rat anti-mouse CD68 (Clone: FA-11, AbD Serotec), goat anti-mouse Gpnmb (Clone: #297310, R&D Systems, Minneapolis, MN, USA), and monoclonal antibody against α-SMA (Clone: E184, Merck Millipore, Billerica, MA, USA). For immunofluorescent staining, paraffin sections were incubated with rat anti-mouse CD68 (Clone: FA-11, AbD Serotec) and goat anti-mouse Gpnmb (Clone: #297310, R&D Systems). These samples then imaged with fluorescent microscopy. The terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick-end labeling assay (Promega, Madison, WI, USA) was performed on paraffin liver sections according to the manufacturer’s instructions. All samples undergoing immunofluorescent staining were labeled with ProLongR Gold Antifade Mountant with DAPI (Thermo Fisher Scientific Inc., Grand Island, NY, USA). Serum levels of alanine aminotransferase (ALT) were measured with a commercial kit (SRL, Tokyo, Japan).

Kumagai K., Tabu K., Sasaki F., Takami Y., Morinaga Y., Mawatari S., Hashimoto S., Tanoue S., Kanmura S., Tamai T., Moriuchi A., Uto H., Tsubouchi H, & Ido A. (2015). Glycoprotein Nonmetastatic Melanoma B (Gpnmb)-Positive Macrophages Contribute to the Balance between Fibrosis and Fibrolysis during the Repair of Acute Liver Injury in Mice. PLoS ONE, 10(11), e0143413.

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Antibody Panel for Neurodegenerative Markers

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The following antibodies were used in this study: mouse anti-Galectin-3 (BioLegend, 126702), goat anti-human GPNMB (R&D Systems, AF2550), goat anti-mouse GPNMB (R&D Systems, AF2330), rat anti-mouse LAMP1 (BD Biosciences, 553792), rabbit anti-IBA-1 (Wako, 01919741), goat anti-AIF-1/Iba1 (Novus Biologicals, NB100–1028), rat anti-CD68 (Bio-Rad, MCA1957), and sheep anti-PGRN (R&D Systems, AF2557), mouse anti-MBP (Millipore, SMI-99), Goat anti-Olig2 (R&D Systems, AF2418), mouse anti-APC (Millipore, OP80), rabbit anti-Perilipin2 (Proteintech Group,15294-1-AP), sheep anti-TREM2 (R&D Systems, AF1729). Detailed information is provided in Supplementary Table 1.
The following reagents were also used in the study: Dulbecco’s modified Eagle’s medium (DMEM)(Cellgro, 10–017-CV), Hanks’ Balanced Salt Solution (HBSS) (Cellgro, 21–020-CV), Dulbecco’s modified Eagle’s medium/Ham’s F-12 (DMEM/F-12)(Cellgro, 10–092-CV), 0.25% Trypsin (Corning, 25–053-CI), Autofluorescence Quencher (Biotium, 23007), Odyssey blocking buffer (LI-COR Biosciences, 927–40000), and O.C.T compound (Electron Microscopy Sciences, 62550–01).

Zhang T., Feng T., Wu K., Guo J., Nana A.L., Yang G., Seeley W.W, & Hu F. (2023). Progranulin deficiency results in sex-dependent alterations in microglia in response to demyelination. Acta neuropathologica, 146(1), 97-119.

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