Samples for Mössbauer spectroscopy require the incorporation of the isotope 57Fe into the Asp1365−920 protein, and thus, the protein was isolated aerobically in the apo form and maturated using chemical reconstitution as described above. Mössbauer spectra were recorded on a conventional spectrometer with alternating constant acceleration cooled with an Oxford Instruments Variox cryostat, using a 57Co/Rh (1.8 GBq) γ-source. Samples were measured at 80 K with no applied magnetic field. Isomer shifts are relative to iron metal at 300 K. Spectra were simulated and fitted using Lorentzian quadrupole doublets using the in-house software MFIT (developed by Eckhard Bill).
Variox cryostat
Manufactured by Oxford Instruments
The Variox cryostat is a versatile laboratory equipment designed for cryogenic temperature control. It provides a stable and precise temperature environment for a variety of applications, including materials research, quantum computing, and superconductivity studies.
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Lab products found in correlation
4 protocols using variox cryostat
1
Mössbauer Spectroscopy of Asp1 Protein
2
Mössbauer Spectroscopy of Frozen Solutions
3
Mössbauer Spectroscopy Characterization
4
Mössbauer Spectroscopy of Solid Samples
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Mössbauer
spectra were recorded on a conventional spectrometer with an alternating
constant acceleration of the γ-source. The minimum experimental
line width was 0.24 mm/s (full width at half-height). The sample temperature
was maintained constant in an Oxford Instruments Variox cryostat.
The 57Co/Rh source (0.6 GBq) was kept at room temperature.
Isomer shifts are quoted relative to iron metal at 300 K. Zero field
spectra were measured at 80 K. For solid state measurements, the obtained
solid was filled into a Delrin capsule in the glovebox. The capsules
were frozen in liquid nitrogen directly after discharging from the
glovebox. Handling and mounting of the samples was performed under
liquid nitrogen. Data simulation was carried out using the program
“mf.SL” written by E. Bill (Max Planck Institute for
Chemical Energy Conversion, Mülheim an der Ruhr).
spectra were recorded on a conventional spectrometer with an alternating
constant acceleration of the γ-source. The minimum experimental
line width was 0.24 mm/s (full width at half-height). The sample temperature
was maintained constant in an Oxford Instruments Variox cryostat.
The 57Co/Rh source (0.6 GBq) was kept at room temperature.
Isomer shifts are quoted relative to iron metal at 300 K. Zero field
spectra were measured at 80 K. For solid state measurements, the obtained
solid was filled into a Delrin capsule in the glovebox. The capsules
were frozen in liquid nitrogen directly after discharging from the
glovebox. Handling and mounting of the samples was performed under
liquid nitrogen. Data simulation was carried out using the program
“mf.SL” written by E. Bill (Max Planck Institute for
Chemical Energy Conversion, Mülheim an der Ruhr).
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