Cd25 bv605

Manufactured by Thermo Fisher Scientific

The CD25-BV605 is a fluorescently-labeled antibody that binds to the CD25 antigen, also known as the IL-2 receptor alpha chain. This product is intended for use in flow cytometry applications to identify and quantify cells expressing the CD25 surface marker.

Automatically generated - may contain errors

Lab products found in correlation

B220 fitc, by Thermo Fisher Scientific (1 mentions) Fcs express 7, by De Novo Software (1 mentions) Foxp3 staining buffer kit, by Thermo Fisher Scientific (1 mentions) Cd4 e450, by Thermo Fisher Scientific (1 mentions) Lsrii flow cytometer, by BD (1 mentions) Foxp3 apc, by Thermo Fisher Scientific (1 mentions) Ifn γ pe, by BD (1 mentions) Cd8 apc, by BioLegend (1 mentions) Tnf α fitc, by BD (1 mentions) Leukocyte activation cocktail, by BD (1 mentions) Alexa 488, by BioLegend (1 mentions) Cd4 percp cy5, by BD (1 mentions) Il4 alexafluor 488, by BioLegend (1 mentions) Cd69 apc cy7, by BD (1 mentions)

2 protocols using cd25 bv605

Regulatory T cell Immunophenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols

Peripheral blood mononuclear cells (PBMCs) were stained with the following antibodies, B220-FITC, CD4-e450, CD8-BV510, CD25-BV605, and FoxP3-e660 using the FoxP3 staining buffer kit from eBioscience per manufacturer's instructions. Data were collected on an Attune NXT flow cytometer and cell population analysis was conducted using FCS Express 7 (De Novo software, Glendale, CA).

Biswas M., Palaschak B., Kumar S.R., Rana J, & Markusic D.M. (2020). B Cell Depletion Eliminates FVIII Memory B Cells and Enhances AAV8-coF8 Immune Tolerance Induction When Combined With Rapamycin. Frontiers in Immunology, 11, 1293.

+ Open protocol

+ Expand

Multiparameter Flow Cytometry of KEAP1-Edited T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fluorochrome conjugated antibodies to following human antigens were used for flow cytometric analysis of KEAP1 edited cells: TCR-BV421(BioLegend, San Diego, CA), CD4-PerCP-Cy5.5 (BD Biosciences, Franklin Lakes, NJ), CD8-APC (BioLegend, San Diego, CA), CD25-BV605 (eBioscience, San Diego, CA), FoxP3-APC (eBioscience, San Diego, CA), or Alexa488 (BioLegend, San Diego, CA) CD69-APC-Cy7 (BD Biosciences, Franklin Lakes, NJ), IFNγ-PE (BD Biosciences, Franklin Lakes, NJ), TNFα-FITC (BD Biosciences, Franklin Lakes, NJ), IL4-AlexaFluor 488 (BioLegend, San Diego, CA) IL-10-PE or APC (eBioscience, San Diego, CA), and IL17-BV421 or PE (BioLegend, San Diego, CA). T lymphocytes (~5×10⁵) were stimulated with leukocyte activation cocktail (BD Pharmigen, San Jose, CA) containing PMA (Phorbol 12-Myristate 13-Acetate), ionomycin and brefeldin A before staining for surface markers and intracellular cytokines. Labelled samples were analyzed with LSRII flow cytometer (BD Biosciences, Franklin Lakes, NJ). Unstained and unstimulated samples were used to correctly identify and gate cell populations during analysis using FlowJo software (Tree Star Inc., Ashland, OR).

Noel S., Lee S.A., Sadasivam M., Hamad A.R, & Rabb H. (2018). KEAP1 editing using CRISPR/Cas9 for therapeutic NRF2 activation in primary human T lymphocytes. Journal of immunology (Baltimore, Md. : 1950), 200(5), 1929-1936.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!