Rutin quercetin 3 o rutinoside hydrate

Catechin hydrate (>98%), trans-resveratrol (>99%), quercetin 3-O-glucuronide (≥95%), cyanin chloride (≥90%), ferulic acid (99%), kaempferol, p-coumaric acid, kaempferol 3-O-rutinoside (98%), myricetin, isorhamnetin and rutin (quercetin 3-O-rutinoside) hydrate (>94%) were obtained from Sigma-Aldrich (Darmstadt, Germany). Sodium carbonate anhydrous (99%), Folin–Ciocalteu reagent, and glycerol anhydrous (99%) were purchased from Penta (Praha, Czechia). Formic acid (99%), L-ascorbic acid and citric acid (≥99.5%) were from Carlo Erba (Milan, Italy). All solvents used for chromatography were of appropriate (HPLC) grade. The radical probe 2,2-diphenyl-1-picrylhydrazyl (DPPH), quercetin hydrate (97%) and caffeic acid (99%) were from Alfa Aesar (Karlsruhe, Germany). Iron chloride hexahydrate (FeCl₃ • 6H₂O) was purchased from Merck (Darmstadt, Germany). 2,4,6-Tris(2-pyridyl)-s-triazine (TPTZ) was from Fluka (Steinheim, Germany).

Gallic acid hydrate was from Panreac (Barcelona, Spain). Glycine (99.5%) was from Applichem (Darmstadt, Germany). Iron chloride hexahydrate was from Merck (Darmstadt, Germany). L-Lactic acid, ascorbic acid (99.5%), sodium acetate trihydrate, sodium carbonate anhydrous (99%) and aluminum chloride anhydrous (98%) were from Penta (Praha, Czechia). Chlorogenic acid (95%) was from Fluorochem (Hadfield, UK). NeoChlorogenic acid (≥98%) was from Merck (Darmstadt, Germany). Folin-Ciocalteu reagent, rutin (quercetin 3-O-rutinoside) hydrate (>94%), isorhamnetin 3-O-glucoside and 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ) were from Sigma-Aldrich (St. Louis, MO, USA).

Glycine (99.5%) was from Applichem (Darmstadt, Germany). Iron chloride hexahydrate was from Merck (Darmstadt, Germany). Rutin (quercetin 3-O-rutinoside) hydrate, kaempferol 3-O-glucoside, 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), 2,2-diphenylpicrylhydrazyl (DPPH) and Folin-Ciocalteu reagent were from Sigma-Aldrich (St. Louis, MO, USA). L-Lactic acid, sodium carbonate anhydrous (99%), ascorbic acid (99.5%), and sodium acetate trihydrate and aluminium chloride anhydrous (98%) were from Penta (Praha, Czechia). Gallic acid hydrate was from Panreac (Barcelona, Spain). Pelargonin (pelargonidin 3,5-di-O-glucoside) chloride was from Extrasynthese (Genay, France).