In vitro EBV infection assay was performed as described previously 15 (link),23 (link). Briefly, EBV was prepared from culture medium of B95-8 cells, and then concentrated (200-fold) in RPMI medium 1640 supplemented with 10% FCS. The virus suspension was filtered (0.45 μm) and the recipient cells (2 × 106–1 × 107) were incubated in 1 or 5 mL of the suspension for 1 h, and then rinsed twice with culture medium (10% RPMI). For inactivation of the EBV genome, 1 mL of virus suspension in a 100-mm dish was irradiated with UV (254 nm) at 1 Jμ/cm2 using a FUNA-UV-LINKER FS-800 (Funakoshi, Tokyo, Japan). Infection was verified by EBV–DNA quantitation, and immune fluorescence staining of EBV nuclear antigen (EBNA) staining of the cells as described using Polyclonal Rabbit Anti-Human C3c Complement/FITC antibody (Dako, Glostrup, Denmark) 24 (link).
Funa uv linker fs 800
Manufactured by Funakoshi
Sourced in Japan
The FUNA-UV-LINKER FS-800 is a UV crosslinking device designed for applications that require controlled UV exposure. The device features adjustable time and intensity settings to enable precise control over the UV treatment process. No further details are provided about the intended use or capabilities of this product.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using funa uv linker fs 800
1
In vitro EBV Infection Assay
2
EBV Infection of MOLT4 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MOLT4 cells were infected with EBV as described previously [26] (link). Briefly, EBV was prepared from culture medium of B95-8 cells as described [51] (link), and then concentrated (200-fold) in RPMI medium 1640 supplemented with 10% FCS. The virus suspension was filtered (0.45 µm) and the recipient cells (2×106 to 1×107) were incubated in 1 or 5 ml of the suspension for 1 h, and then rinsed twice with culture medium (10% RPMI). The efficiency of infection was >90% as judged by EBNA1 staining. For inactivation of the EBV genome, 1 ml of virus suspension in a 100-mm dish was irradiated with UV (254 nm) at 1 Jµcm2 using a FUNA-UV-LINKER FS-800 (Funakoshi, Tokyo). Infection was verified by EBV DNA quantification, and immune fluorescence staining of EBNA1 staining of the cells as described using Polyclonal Rabbit Anti-Human C3c Complement/FITC antibody (Dako, Glostrup, Denmark) [52] (link).
3
Southern Blotting of Genomic DNA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Southern blotting was performed as follows. Briefly, genomic DNA that was isolated from ES cells and digested with BamHI or HindIII was resolved on 0.7% agarose gels, transferred to Hybond-N+ membrane (GE Healthcare Life Sciences), and was exposed under the UV light using a UV crosslinker (FUNA-UV-LINKER FS-800, Funakoshi). Next, the membrane was hybridized with 5’, 3’, and neomycin probes labeled with DIG-dUTP at 40°C overnight, incubated with stringency solution and hybridized with Anti-Digoxigenin-AP, Fab fragments (Roche). Signals were detected using CDP-Star (Roche) in automatic processor (Fujifilm).
4
UV Cross-linking of RNA-Protein Complexes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The 32P-labeled RNAs (3000 cpm/μl) were mixed with purified recombinant His-T7-C1 and/or His-CBC in a 20 μl volume containing 8 mM Tris–HCl (pH 8.0), 125 mM NaCl, 1.6 mM MgCl2, 4% glycerol and RNasin. After an incubation at 30°C for 15 min, the mixture was irradiated by 254 nm UV light (FUNA-UV-LINKER FS-800; Funakoshi, Tokyo, Japan) at 100 mJ/cm2 on ice. The irradiated sample was treated with RNase A (1 mg/ml; Nacalai Tesque) at 37°C for 15 min and cross-linked proteins were analyzed by SDS–PAGE and autoradiography.
5
UV Radiation Inactivation of F. tularensis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Five microliters of F. tularensis SCHU P9 spiked in 100 μL of deionized water, CDM or undiluted FBS were transferred into 1.5 mL tubes (Sarstedt K.K., Tokyo, Japan) and 0.2 mL PCR tubes (Bio-Bik Ina Optica). These samples were radiated at room temperature (23°C) using a Funa-UV-Linker (FS-800; Funakoshi, Tokyo, Japan) equipped with a low-pressure lamp (254 nm). The average UV energy (3 mW/cm2) was monitored with the sensor of this system during this experiment. After radiation, the CFU in 100 μL of these samples was measured. These experiments were conducted using four replicates.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!