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Alpha lytic protease

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Alpha lytic protease is a microbial serine protease derived from the bacterium Lysobacter enzymogenes. It is a highly active enzyme that exhibits a broad specificity for cleaving peptide bonds.

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Lab products found in correlation

Trypsin, by Promega (2 mentions) Asp n, by Promega (2 mentions) Glu c, by Promega (1 mentions) 18o water, by Cambridge Isotopes (1 mentions) Endoglycosidase h, by Promega (1 mentions) Simplyblue safestain, by Thermo Fisher Scientific (1 mentions) Iodoacetamide, by Merck Group (1 mentions) Dithiothreitol (dtt), by Merck Group (1 mentions) Pngase f, by Promega (1 mentions) Chymotrypsin, by Promega (1 mentions) Lys c, by Promega (1 mentions)

2 protocols using alpha lytic protease

1

Gel Electrophoresis and Protein Digestion

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4–15% Mini-PROTEAN® TGX™ Precast Protein Gels, 15-well, 15 μl (Bio-Rad); SimplyBlue™ SafeStain (Invitrogen); dithiothreitol (Sigma); iodoacetamide (Sigma); alpha lytic protease (New England BioLabs); chymotrypsin (Athens Research and Technology); AspN (Promega); Glu-C (Promega); trypsin (Promega); endoglycosidase H (Promega); PNGaseF (Promega); 18O water (Cambridge Isotope Laboratories).
Chmielewski D., Wilson E.A., Pintilie G., Zhao P., Chen M., Schmid M.F., Simmons G., Wells L., Jin J., Singharoy A, & Chiu W. (2023). Integrated analyses reveal a hinge glycan regulates coronavirus spike tilting and virus infectivity. Research Square.
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2

SARS-CoV-2 Spike Protein Characterization

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All chemicals were obtained from Sigma–Aldrich (St. Louis, MO) unless indicated otherwise. Endoproteases used in this study, including trypsin (Cat#XV528X, Mass Spectrometry Grade), chymotrypsin (Cat#V1061, Sequencing Grade), Lys-C (Cat#VA1170, Mass Spectrometry Grade), and Asp-N (Cat#V162A, Sequencing Grade), were obtained from Promega (Madison, WI), and alpha-lytic protease (Cat#P8113S, Proteomics Grade) was purchased from New England BioLabs (Ipswich, MA). The recombinant poly-histidine tagged, trimeric ectodomains of the SARS-CoV-2 S variants (D614G, Alpha, Beta, Gamma, and Delta) expressed in HEK293 cells were purchased from R&D Systems (Minneapolis, MN). The sequences of the two S proteins included a mutated furin cleavage site and K986P, V987P (2P) substitutions.
Wang D., Baudys J., Osman S.H, & Barr J.R. (2023). Analysis of the N-glycosylation profiles of the spike proteins from the Alpha, Beta, Gamma, and Delta variants of SARS-CoV-2. Analytical and Bioanalytical Chemistry, 415(19), 4779-4793.
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