Phenotypical characterization of ASCs was performed using the BD FACSCalibur and analyzed with the CellQuest software (v. 1.0.1, Becton–Dickinson, Heidelberg, Germany). The cells were trypsinized, placed on ice for 30 min, and treated with the following labeled stemness-associated antigen markers: CD31-, CD34+, CD45–, CD54–, CD90+, CD105+, CD166+, HLA-ABC+, HLA-DR–. CD34-PE, CD90–FITC, and CD105-PerCP were part of the BD Stemflow Kit (Becton Dickinson, 562245). The other antibodies with the indicated specifications were purchased separately: CD31-FITC (R & D, Systems, Wiesbaden, Germany, FAB3567F), HLA-DR-PE (Becton Dickinson, 347401), CD166-PE (Becton Dickinson, 559263), CD34-APC (Becton Dickinson, 555824), CD54-FITC (Beckman Coulter, Krefeld, Germany, PN IM0726U), HLA-ABC-FITC (Becton Dickinson, 555552), and CD45-PE (Becton Dickinson, 555483). Table 1 shows the results from three donors. All experiments shown in this paper were performed with cells until reaching passage 5.
Cd31 fitc
Manufactured by R&D Systems
Sourced in Germany
CD31-FITC is a fluorescent-labeled antibody that recognizes the CD31 cell surface antigen. CD31, also known as PECAM-1, is a transmembrane glycoprotein expressed on the surface of endothelial cells, platelets, and some leukocytes. This antibody can be used for the identification and enumeration of cells expressing CD31 in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Cellquest software, by BD (1 mentions)
Cd105, by BD (1 mentions)
Hla dr pe, by BD (1 mentions)
Cd45 pe, by BD (1 mentions)
Cd90 fitc, by BD (1 mentions)
Cd34 apc, by BD (1 mentions)
Cd54 fitc, by Beckman Coulter (1 mentions)
Hla abc fitc, by BD (1 mentions)
Cd34 pe, by BD (1 mentions)
Cd166 pe, by BD (1 mentions)
Facscalibur, by BD (1 mentions)
Streptavidin apc, by Thermo Fisher Scientific (1 mentions)
Pdpn apc, by Thermo Fisher Scientific (1 mentions)
Cd26 percp cy5, by Thermo Fisher Scientific (1 mentions)
Fap biotin, by R&D Systems (1 mentions)
α sma cy3, by Merck Group (1 mentions)
Cd8 bv605, by BD (1 mentions)
Ly6c percpcy5, by R&D Systems (1 mentions)
Pd l1 apc, by BD (1 mentions)
F4 80 fitc, by R&D Systems (1 mentions)
2 protocols using cd31 fitc
1
Phenotypic Characterization of ASCs
2
Comprehensive Immune Cell Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following antibodies/dies (purchased from BioLegend unless otherwise stated) were used for flow cytometry: CD45-PE-Cy7, CD31-FITC, FAP-biotin (R&D system), streptavidin-APC, CD90-BV605, PDPN-APC, Ly6C-AF700, CD26-PerCP-Cy5.5 (eBioscience), αSMA-Cy3 (Sigma), CD11b-PE-Cy7, CD11b-Pacific Blue, F4/80-APC, F4/80-FITC, MR-PE, MR-PE-Cy7, Ly6C-PerCP-Cy5.5, Ly6G-APC-Cy7, Arg1-PE (R&D system), PDL1-APC (BD Biosciences), CD45-FITC, CD3-AF700, CD4-BV421, CD8-BV605 (BD Biosciences), CD25-PE-Cy7, FoxP3-APC and carboxyfluorescein succinimidyl ester (CFSE, Sigma Aldrich). Viability was assessed with Zombie Aqua (BioLegend). Samples were Fc receptor-blocked using mouse FcR blocking reagent (1:10; Miltinyi Biotec). For intracellular staining, samples were fixated and permeabilized with eBioscience Fixation/Permeabilization Concentrate, Diluent and 10X Buffer (Invitrogen). Data were acquired on FACSCanto II (BD Biosciences) or ACEA NovoCyte Quanteon (Agilent) and analyzed with FlowJo V.10.6.1 (Tree Star). Gating strategies can be found in online supplemental figures 8–13 .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!