Normal and GO-derived OFs (5 × 103/cm2) were cultured in a six-well plate and incubated in serum-free DMEM/F12 supplemented with 33 μM biotin, 17 μM pantothenic acid, 0.2 nM triiodothyronine (T3), 10 μg/mL transferrin, 0.2 μM carbaprostacyclin (cPGI2; Cayman Chemical, Ann Arbor, MI, USA), 0.1 mM isobutylmethylxanthine (IBMX), 1 μM dexamethasone, and 1 μM insulin (Sigma-Aldrich). The differentiation-induced medium was replaced every day for 4 days. The medium exchanged to a maturation medium without 1 μM dexamethasone and 0.1 mM IBMX (all from Sigma-Aldrich) for 6 days and was replaced every other day. Lipid droplets were stained using 0.5 μg/mL Nile red solution (Sigma-Aldrich).
Carbaprostacyclin cpgi 2
Manufactured by Cayman Chemical
Sourced in United States
Carbaprostacyclin (cPGI 2) is a laboratory equipment product manufactured by Cayman Chemical. It is a synthetic analogue of the natural prostacyclin molecule. The core function of cPGI 2 is to serve as a research tool for investigating the biological activities of prostacyclin.
Automatically generated - may contain errors
Lab products found in correlation
Isobutylmethylxanthine ibmx, by Merck Group (5 mentions)
3 isobutyl 1 methyl xanthine (ibmx), by Merck Group (5 mentions)
Insulin, by Merck Group (5 mentions)
Dexamethasone (dex), by Merck Group (5 mentions)
Stempro adipogenesis and osteogenesis differentiation kit, by Thermo Fisher Scientific (4 mentions)
Oil red o, by Merck Group (4 mentions)
Silver nitrate, by Merck Group (4 mentions)
Transferrin, by Merck Group (4 mentions)
Triiodothyronine t3, by Merck Group (3 mentions)
Nile red solution, by Merck Group (1 mentions)
Pantothenic acid, by Merck Group (1 mentions)
5 protocols using carbaprostacyclin cpgi 2
1
Adipocyte Differentiation Protocol
2
Differentiation of PD-MSCs into Mesodermal Lineages
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To analyze the potential of PD-MSCsPRL-1 to differentiate into mesodermal lineages, PD-MSCsPRL-1 (passage = 5) were plated at a density of 5 × 103 cells/cm2 in various differentiation induction media using the StemPro adipogenesis and osteogenesis differentiation kit (Gibco) according to the manufacturer’s instructions. After approximately 21 days, PD-MSCsPRL-1 were fixed in 4% paraformaldehyde and incubated for 1 h with Oil Red O (Sigma-Aldrich) to stain lipids to visualize lipid vesicles and von Kossa with 5% silver nitrate (Sigma-Aldrich) under the light to evaluate the accumulation of calcium deposits.
To induce adipogenic differentiation, normal and GO-derived OFs (5 × 103 cells/cm2) were seeded and incubated in serum-free DMEM/F12 supplemented with 33 μM biotin, 17 μM pantothenic acid, 10 μg/mL transferrin, 0.2 nM triiodothyronine (T3), 1 μM insulin (all from Sigma-Aldrich), 0.2 μM carbaprostacyclin (cPGI2; Cayman Chemical, Ann Arbor, MI, USA), 1 μM dexamethasone, and 0.1 mM isobutylmethylxanthine (IBMX; all from Sigma-Aldrich) for the first 4 days. To induce the maturation of adipocytes, the medium was supplemented except 1 μM dexamethasone and 0.1 mM IBMX (all from Sigma-Aldrich) for 6 days and was replaced every other day. Lipid accumulation and adipocyte morphology were visualized by Oil Red O staining.
To induce adipogenic differentiation, normal and GO-derived OFs (5 × 103 cells/cm2) were seeded and incubated in serum-free DMEM/F12 supplemented with 33 μM biotin, 17 μM pantothenic acid, 10 μg/mL transferrin, 0.2 nM triiodothyronine (T3), 1 μM insulin (all from Sigma-Aldrich), 0.2 μM carbaprostacyclin (cPGI2; Cayman Chemical, Ann Arbor, MI, USA), 1 μM dexamethasone, and 0.1 mM isobutylmethylxanthine (IBMX; all from Sigma-Aldrich) for the first 4 days. To induce the maturation of adipocytes, the medium was supplemented except 1 μM dexamethasone and 0.1 mM IBMX (all from Sigma-Aldrich) for 6 days and was replaced every other day. Lipid accumulation and adipocyte morphology were visualized by Oil Red O staining.
3
Differentiation of PD-MSCs PRL-1 into Mesodermal Lineages
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To analyze the potential of PD-MSCs PRL-1 to differentiate into mesodermal lineages, PD-MSCs PRL-1 (passage = 5) were plated at a density of 5×10 3 cells/cm 2 in various differentiation induction media using the StemPro adipogenesis and osteogenesis differentiation kit (Gibco) according to the manufacturer's instructions. After approximately 21 days, PD-MSCs PRL-1 were xed in 4% paraformaldehyde and incubated for 1 h with Oil Red O (Sigma-Aldrich) to stain lipids to visualize lipid vesicles and von Kossa with 5% silver nitrate (Sigma-Aldrich) under the light to evaluate the accumulation of calcium deposits.
To induce adipogenic differentiation, normal and GO-derived OFs (5×10 3 cells/cm 2 ) were seeded and incubated in serum-free DMEM/F12 supplemented with 33 μM biotin, 17 μM pantothenic acid, 10 μg/mL transferrin, 0.2 nM triiodothyronine (T 3 ), 1 μM insulin (all from Sigma-Aldrich), 0.2 μM carbaprostacyclin (cPGI 2 ; Cayman Chemical, Ann Arbor, MI, USA), 1 μM dexamethasone, and 0.1 mM isobutylmethylxanthine (IBMX; all from Sigma-Aldrich) for the rst 4 days. To induce the maturation of adipocytes, the medium was supplemented except 1 μM dexamethasone and 0.1 mM IBMX (all from Sigma-Aldrich) for 6 days and was replaced every other day. Lipid accumulation and adipocyte morphology were visualized by Oil Red O staining.
To induce adipogenic differentiation, normal and GO-derived OFs (5×10 3 cells/cm 2 ) were seeded and incubated in serum-free DMEM/F12 supplemented with 33 μM biotin, 17 μM pantothenic acid, 10 μg/mL transferrin, 0.2 nM triiodothyronine (T 3 ), 1 μM insulin (all from Sigma-Aldrich), 0.2 μM carbaprostacyclin (cPGI 2 ; Cayman Chemical, Ann Arbor, MI, USA), 1 μM dexamethasone, and 0.1 mM isobutylmethylxanthine (IBMX; all from Sigma-Aldrich) for the rst 4 days. To induce the maturation of adipocytes, the medium was supplemented except 1 μM dexamethasone and 0.1 mM IBMX (all from Sigma-Aldrich) for 6 days and was replaced every other day. Lipid accumulation and adipocyte morphology were visualized by Oil Red O staining.
4
Differentiation of PD-MSCs PRL-1 into Mesodermal Lineages
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To analyze the potential of PD-MSCs PRL-1 to differentiate into mesodermal lineages, PD-MSCs PRL-1 (passage = 5) were plated at a density of 5×10 3 cells/cm 2 in various differentiation induction media using the StemPro adipogenesis and osteogenesis differentiation kit (Gibco) according to the manufacturer's instructions. After approximately 21 days, PD-MSCs PRL-1 were xed in 4% paraformaldehyde and incubated for 1 h with Oil Red O (Sigma-Aldrich) to stain lipids to visualize lipid vesicles and von Kossa with 5% silver nitrate (Sigma-Aldrich) under the light to evaluate the accumulation of calcium deposits.
To induce adipogenic differentiation, normal and GO-derived OFs (5×10 3 cells/cm 2 ) were seeded and incubated in serum-free DMEM/F12 supplemented with 33 μM biotin, 17 μM pantothenic acid, 10 μg/mL transferrin, 0.2 nM triiodothyronine (T 3 ), 1 μM insulin (all from Sigma-Aldrich), 0.2 μM carbaprostacyclin (cPGI 2 ; Cayman Chemical, Ann Arbor, MI, USA), 1 μM dexamethasone, and 0.1 mM isobutylmethylxanthine (IBMX; all from Sigma-Aldrich) for the rst 4 days. To induce the maturation of adipocytes, the medium was supplemented except 1 μM dexamethasone and 0.1 mM IBMX (all from Sigma-Aldrich) for 6 days and was replaced every other day. Lipid accumulation and adipocyte morphology were visualized by Oil Red O staining.
To induce adipogenic differentiation, normal and GO-derived OFs (5×10 3 cells/cm 2 ) were seeded and incubated in serum-free DMEM/F12 supplemented with 33 μM biotin, 17 μM pantothenic acid, 10 μg/mL transferrin, 0.2 nM triiodothyronine (T 3 ), 1 μM insulin (all from Sigma-Aldrich), 0.2 μM carbaprostacyclin (cPGI 2 ; Cayman Chemical, Ann Arbor, MI, USA), 1 μM dexamethasone, and 0.1 mM isobutylmethylxanthine (IBMX; all from Sigma-Aldrich) for the rst 4 days. To induce the maturation of adipocytes, the medium was supplemented except 1 μM dexamethasone and 0.1 mM IBMX (all from Sigma-Aldrich) for 6 days and was replaced every other day. Lipid accumulation and adipocyte morphology were visualized by Oil Red O staining.
5
Mesenchymal Stem Cell Differentiation Potential
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To analyze the potential of PD-MSCs PRL-1 to differentiate into mesodermal lineages, PD-MSCs PRL-1 (passage = 5) were plated at a density of 5×10 3 cells/cm 2 in various differentiation induction media using the StemPro adipogenesis and osteogenesis differentiation kit (Gibco) according to the manufacturer's instructions. After approximately 21 days, PD-MSCs PRL-1 were xed in 4% paraformaldehyde and incubated for 1 h with Oil Red O (Sigma-Aldrich) to stain lipids to visualize lipid vesicles and von Kossa with 5% silver nitrate (Sigma-Aldrich) under the light to evaluate the accumulation of calcium deposits.
To induce adipogenic differentiation, normal and GO-derived OFs (5×10 3 cells/cm 2 ) were seeded and incubated in serum-free DMEM/F12 supplemented with 33 μM biotin, 17 μM pantothenic acid, 10 μg/mL transferrin, 0.2 nM triiodothyronine (T 3 ), 1 μM insulin (all from Sigma-Aldrich), 0.2 μM carbaprostacyclin (cPGI 2 ; Cayman Chemical, Ann Arbor, MI, USA), 1 μM dexamethasone, and 0.1 mM isobutylmethylxanthine (IBMX; all from Sigma-Aldrich) for the rst 4 days. To induce the maturation of adipocytes, the medium was supplemented except 1 μM dexamethasone and 0.1 mM IBMX (all from Sigma-Aldrich) for 6 days and was replaced every other day. Lipid accumulation and adipocyte morphology were visualized by Oil Red O staining.
To induce adipogenic differentiation, normal and GO-derived OFs (5×10 3 cells/cm 2 ) were seeded and incubated in serum-free DMEM/F12 supplemented with 33 μM biotin, 17 μM pantothenic acid, 10 μg/mL transferrin, 0.2 nM triiodothyronine (T 3 ), 1 μM insulin (all from Sigma-Aldrich), 0.2 μM carbaprostacyclin (cPGI 2 ; Cayman Chemical, Ann Arbor, MI, USA), 1 μM dexamethasone, and 0.1 mM isobutylmethylxanthine (IBMX; all from Sigma-Aldrich) for the rst 4 days. To induce the maturation of adipocytes, the medium was supplemented except 1 μM dexamethasone and 0.1 mM IBMX (all from Sigma-Aldrich) for 6 days and was replaced every other day. Lipid accumulation and adipocyte morphology were visualized by Oil Red O staining.
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