Rpmi essential medium

The human breast cancer cell line MDA-MB 231 was obtained from the American Type Culture Collection (ATCC HTB-26). Cells were routinely grown in Roswell Park Memorial Institute (RPMI) essential medium (Sigma Aldrich, St. Louis, MO, USA) containing 10% fetal bovine serum (LGC Biotechnology, Cotia, SP, Brazil) and 1% penicillin–streptomycin (Sigma Aldrich, St. Louis, MO, USA) in a humidified 5% CO₂ atmosphere at 37 °C. Cells were cultured up to 70–100% confluence. The compounds were dissolved in dimethyl sulfoxide (DMSO) (Sigma Aldrich, St. Louis, MO, USA) in a 1 mM stock solution, and the concentrations used were dissolved in RPMI at different concentrations. The DMSO concentration did not exceed 0.1% in the final solution and had no effect per se.

The human prostate cancer cell line PC3 was obtained from the American Type Culture Collection (ATCC^® CRL-1435™). The cells were routinely grown in Roswell Park Memorial Institute (RPMI) essential medium (Sigma-Aldrich, St. Louis, MO, USA) containing 10% fetal bovine serum (LGC Biotechnology, São Paulo, Brazil) and 1% penicillin–streptomycin (Sigma Aldrich, St. Louis, MO, USA) in a humidified 5% CO₂ atmosphere at 37 °C. The cells were cultured to 70–100% confluence. The compounds were dissolved in dimethyl sulfoxide (DMSO) (Sigma-Aldrich, St. Louis, MO, USA) to produce a 10 mM stock solution, and the compounds were dissolved in RPMI at different concentrations. The DMSO concentration did not exceed 0.1% and did not have any effect.