The HCT116 cell line was obtained from Procell (CVCL_B8AV, Wuhan, China) and cultured in complete McCoy’s 5A medium (PM150710B, Procell). Human CRC cell lines HCT8 (CVCL_2478), LOVO (CVCL_0399), SW480 (CVCL_0546) and normal colon epithelial cell lines NCM460 (CVCL_0460) was obtained from The Cell Bank of the Chinese Academy of Sciences (Shanghai, China). The 293 Phoenix Ampho (293PA) (CVCL_4W26) and HEK-293 (CVCL_0045) cells were generously provided by Prof. Tong-Chuan HE (University of Chicago, Chicago, USA) and they were cultured in Dulbecco’s Modified Eagle’s medium-supplemented with 10% fetal bovine serum (FBS; Hyclone, CA, USA), as well as 100 units of penicillin, and 100 mg of streptomycin and maintained in an incubator with 5% CO2 at 37°C.
Hct116 cell line
Manufactured by Procell
Sourced in China
The HCT116 cell line is a human colorectal carcinoma cell line. It is commonly used in cancer research and drug discovery studies.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Agomir nc, by GenePharma (1 mentions)
Hek293t cells, by Thermo Fisher Scientific (1 mentions)
Dmem medium, by Thermo Fisher Scientific (1 mentions)
Hct116, by Procell (1 mentions)
Mccoy s 5a medium, by Procell (1 mentions)
Ht 29 cell line, by Procell (1 mentions)
3 protocols using hct116 cell line
1
Cell Line Cultivation and Characterization
2
HCT116 Cell Culture Protocol
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Human CC HCT116 was selected in this study. HCT116 cell line was purchased from Procell Life Science and Technology Co., Ltd. (Wuhan, China). The cells were cultured and maintained on the McCoy's 5 A medium supplemented with 10% FBS, 100 U/ml penicillin, and 100 mg/ml streptomycin in a humidified chamber with 5% CO2 at 37°C.
3
Colorectal Cancer Cell Lines Protocol
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HCT116 cell line, SW620 cell line, and HT29 cell line were purchased from Procell (Wuhan, China). HEK 293T cell line was purchased from Zhong Qiao Xin Zhou (Shanghai, China). HCT116 cells and HT29 cells were cultured in McCoy’s 5A medium (Procell, Wuhan, China) containing 10% fetal bovine serum (Hyclone, Logan, Utah, USA). SW620 cells were cultured in RPMI-1640 medium (Gibco, Grand Island, New York, USA) supplemented with 10% fetal bovine serum. HEK 293T cells were maintained in DMEM medium (Gibco, Grand Island, New York, USA) containing 10% fetal bovine serum. All cell lines were cultured in a humidified atmosphere at 37° C with 5% CO2.
NC agomir and miR-370-3p agomir were purchased from GenePharma (Shanghai, China). miR-370-3p inhibitor was purchased from JTS scientific (Wuhan, China). The sequences of β-catenin siRNA (JTS scientific, Wuhan, China) were as follows: forward, 5ʹ-GAUGGUGUCUGCUAUUGUATT-3ʹ, reverse 5ʹ-UACAAUAGCAGACACCAUCTT-3ʹ. The transfection was performed by using Lipofectamine 2000 (Invitrogen, Carlsbad, California, USA) according to the instruction.
NC agomir and miR-370-3p agomir were purchased from GenePharma (Shanghai, China). miR-370-3p inhibitor was purchased from JTS scientific (Wuhan, China). The sequences of β-catenin siRNA (JTS scientific, Wuhan, China) were as follows: forward, 5ʹ-GAUGGUGUCUGCUAUUGUATT-3ʹ, reverse 5ʹ-UACAAUAGCAGACACCAUCTT-3ʹ. The transfection was performed by using Lipofectamine 2000 (Invitrogen, Carlsbad, California, USA) according to the instruction.
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