Jem 2210

Manufactured by JEOL

Sourced in Japan

The JEM-2210 is a high-performance transmission electron microscope (TEM) designed and manufactured by JEOL. It is capable of producing high-resolution images and electron diffraction patterns of various materials at the nanoscale level. The core function of the JEM-2210 is to enable the observation, analysis, and characterization of microscopic structures and compositions of samples.

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Lab products found in correlation

Rf 5301pc, by Shimadzu (2 mentions) Fv1000, by Olympus (2 mentions) Nano z, by Malvern Panalytical (1 mentions) Uv 2550, by Shimadzu (1 mentions) Nano s90, by Malvern Panalytical (1 mentions) Spectramax i3, by Molecular Devices (1 mentions) Cytation 5 plate reader, by Agilent Technologies (1 mentions) Digitalmicrograph camera andsoftware suite, by Ametek (1 mentions) Ht7700 tem, by Hitachi (1 mentions) Zetasizer nano z, by Malvern Panalytical (1 mentions) Lsm 700 confocal microscope, by Zeiss (1 mentions) Advance 300mhz nmr spectrophotometer, by Bruker (1 mentions)

3 protocols using jem 2210

Nanoparticle Characterization via Multimodal Techniques

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TEM image was taken from a transmission electron microscope (JEM-2210, JEOL) and analysed by Gatan DigitalMicrograph software. UV-vis spectra were measured from a UV-vis spectrometer (UV 2550, Shimadzu) and fluorescence spectra were measured from a spectrofluorophotometer (RF-5301 PC, Shimadzu). Hydrodynamic volume was measured from zetasizer (Nano S90, Malvern) at 0.1 mg/mL in phosphate buffered saline (PBS) and zeta potential was measured from zetasizer (Nano Z, Malvern) at 0.1 mg/mL in PBS. Confocal laser scanning microscope (CLSM) image was obtained from Olympus FV-1000 and analysed by OLYMPUS FLUOVIEW ver. 1.5 Viewer software.

Lee J., Lee Y.M., Kim J, & Kim W.J. (2017). Doxorubicin/Ce6-Loaded Nanoparticle Coated with Polymer via Singlet Oxygen-Sensitive Linker for Photodynamically Assisted Chemotherapy. Nanotheranostics, 1(2), 196-207.

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Microscopic Characterization of Nanomaterials

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Transmission electron microscopy (TEM) images were taken using a transmission electron microscope (JEM-2210, JEOL) and analyzed using Gatan DigitalMicrograph software. UV-vis absorbance for the MTT assay was measured using a microplate spectrofluorometer (VICTOR3 V multilabel counter), and fluorescence spectra were obtained from a spectrofluorophotometer (RF-5301 PC, Shimadzu) or multi-well plate reader (SpectraMax^® i3, Molecular Devices). Hydrodynamic size and zeta potential were measured using a Zetasizer (Nano S90, Nano Z, respectively; Malvern) at 0.1 mmol/L in Dulbecco's phosphate-buffered saline (DPBS, pH 7.4) or 0.1 mol/L acetate buffer (pH 5.5). The confocal laser scanning microscope (CLSM) images were obtained using an Olympus FV-1000 and analyzed using the Olympus FluoView ver 1.7 software.

Lee J., Kim J., Lee Y.M., Park D., Im S., Song E.H., Park H, & Kim W.J. (2017). Self-assembled nanocomplex between polymerized phenylboronic acid and doxorubicin for efficient tumor-targeted chemotherapy. Acta Pharmacologica Sinica, 38(6), 848-858.

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Characterization of Nanomaterials Using Advanced Techniques

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TEM images were obtained using an HT7700 TEM (Hitachi, Tokyo, Japan) and JEM-2210 (JEOL, Tokyo, Japan) coupled with the DigitalMicrograph camera and software suite from Gatan (Pleasanton, CA). ¹H-NMR spectra were obtained using a Bruker Advance 300 MHz NMR spectrophotometer (Bruker, MA, USA). Hydrodynamic size of nanomaterials was measured using Zetasizer Nano ZS from Malvern Instruments (Worcestershire, United Kingdom). All UV/vis absorption spectra and fluorescence spectra were measured using a Cytation 5 plate reader from BioTek (Winooski, VT, USA). The CLSM images were observed using a LSM 700 confocal microscope from ZEISS (Oberkochen, Germany).

Sungjin J.u.n.g., Lee J., Lim J., Suh J., Kim T., Ahn J., Kim W.J, & Kim Y. (2020). Polymeric Nanoparticles Controlled by On-chip Self-Assembly Enhance Cancer Treatment Effectiveness. Advanced healthcare materials, 9(22), e2001633.

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