Tcrαβ

Manufactured by Miltenyi Biotec

Sourced in Germany

The TCRαβ is a laboratory equipment designed for the detection and analysis of T cell receptor (TCR) α and β chains. It provides a reliable and efficient method for the characterization of T cell populations.

Automatically generated - may contain errors

Lab products found in correlation

Lsrii cytometer, by BD (1 mentions) Percp cy5.5 conjugated streptavidin, by Thermo Fisher Scientific (1 mentions) Annexin 5 apoptosis detection kit apc, by Thermo Fisher Scientific (1 mentions) Alexa fluor 700 conjugated cd4, by BD (1 mentions) Fix perm, by BD (1 mentions) Aria 2, by BD (1 mentions) Facscalibur, by BD (1 mentions) Tcrγδ, by Miltenyi Biotec (1 mentions)

Close spelling variants of this product

Anti-TCRαβ (2 citations)

3 protocols using tcrαβ

Comprehensive Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following anti-human monoclonal antibodies were used : FITC conjugated CD4, TCRγδ (BD Biosciences), PE-conjugated PD-1, TCRαβ (Miltenyi), CD62L, CCR7 (BD Pharmingen), CD28 (BD Biosciences), PE-cy7 conjugated CD8β (ebiosciences), CD10 (BD biosciences), APC-conjugated CD3, CD5, CD8α, CD56 (BD Biosciences), TCRαβ (Miltenyi), CXCR3 (Biolegend), APC-cy7 conjugated CD3, CD27 (ebiosciences), CD8α (BD biosciences), efluor 450-conjugated CD1a (ebiosciences), Alexafluor 700 conjugated CD4 (BD Pharmingen), biotin-conjugated PD-1 (Miltenyi), PERCP-cy5.5 conjugated Streptavidin (ebiosciences). CD8αα expression was assessed by staining with PE-conjugated TL-tetramer (provided by H. Cheroutre) after prior staining with anti-CD8β antibodies. Annexin V apoptosis detection kit APC (ebiosciences) was used for quantification of apoptosis. Flow cytometric analysis was performed using a LSR II Cytometer (BD Biosciences).

Verstichel G., Vermijlen D., Martens L., Goetgeluk G., Brouwer M., Thiault N., Van Caeneghem Y., De Munter S., Weening K., Bonte S., Leclercq G., Taghon T., Kerre T., Saeys Y., Van Dorpe J., Cheroutre H, & Vandekerckhove B. (2017). The Checkpoint for Agonist Selection precedes Conventional Selection in Human Thymus. Science immunology, 2(8), eaah4232.

+ Open protocol

+ Expand

Multiparameter Flow Cytometry Immunophenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols

Surface marker staining was performed in DPBS with 1% FBS using antibody concentration recommended by the supplier. Intracellular staining was performed following the supplier's protocol using Fix & Perm (BD Biosciences). Flow cytometric analysis was performed on the LSR II and cell sorting on the ARIA II (both BD Biosciences), both equipped with fur lasers. All populations studied were devoid of dead cells based on propidium iodide negativity and of doublets based on FSC-A FSC-W ratios. The following anti-human monoclonal antibodies are CD5 (BD Biosciences, cat# 345782), IgG-Fc (eBioscience, cat# 12-4998-82), Vβ14 (Beckman Coulter, cat# PN IM2047); APC-conjugated: CD8α (BD Biosciences, cat# 345775), TCRαβ (Miltenyi, cat# 130-091-237), CD45 (Miltenyi, cat# 130-091-230), CD34 (BD Biosciences, cat# 345804); Amcyan-conjugated: CD45 (BD Biosciences, cat# 339192); V450-conjugated: CD7 (BD Biosciences, cat# 642916), CD3 (eBioscience, cat# 48-0038-42); APC-Cy7-conjugated: CD27 (eBioscience, cat# 47-0279-42); PE-Cy7-conjugated: CD8β (eBioscience, cat# 25-5273-42); AF-700-conjugated: CD4⁺ (BD Biosciences, cat# 557922); BV510-conjugated: CD45 (BD Biosciences, cat# 563204); biotin-conjugated: CD1a (ATCC); PerCPCy5.5-conjugated: Streptavidin (eBioscience, cat# 45-4317-82).

Van Caeneghem Y., De Munter S., Tieppo P., Goetgeluk G., Weening K., Verstichel G., Bonte S., Taghon T., Leclercq G., Kerre T., Debets R., Vermijlen D., Abken H, & Vandekerckhove B. (2017). Antigen receptor-redirected T cells derived from hematopoietic precursor cells lack expression of the endogenous TCR/CD3 receptor and exhibit specific antitumor capacities. Oncoimmunology, 6(3), e1283460.

+ Open protocol

+ Expand

Comprehensive Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Isolated PBMCs were stained with antibodies against human CD132 (IL-2RG), CD3, CD4, CD8, CD14, CD19, CD56, TCRαβ, and TCRγδ (Miltenyi Biotec, Bergisch Gladbach, Germany). Samples were incubated with the antibodies for 10min at room temperature in the dark and were read by flow cytometer (BD FACS Calibur, BD Biosciences, Franklin Lakes, USA). Data were carried out using FlowJo 10.7.2 software (BD Biosciences, Franklin Lakes, NJ, USA).

Hou Y., Gratz H.P., Ureña-Bailén G., Gratz P.G., Schilbach-Stückle K., Renno T., Güngör D., Mader D.A., Malenke E., Antony J.S., Handgretinger R, & Mezger M. (2021). Somatic Reversion of a Novel IL2RG Mutation Resulting in Atypical X-Linked Combined Immunodeficiency. Genes, 13(1), 35.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!