Sarcoma and breast cancer cell lines were cultured in incubators maintained at 37°C in the presence of 5% CO2. AU565 (cat. no. CRL-2351), HCC70 (cat. no. CRL-2115), and BT-549 (cat. no. HTB-122) human breast cancer lines, and SW872 human liposarcoma cells (cat. no. HTB-92) were grown in RPMI-1640 medium supplemented with 10% fetal bovine serum and penicillin/streptomycin antibiotics (all from ATCC, Manassas, VA, USA). The SK-BR-3 human breast cancer line (cat. no. HTB-30; ATCC) was grown in McCoy's medium supplemented with 10% fetal bovine serum and penicillin/streptomycin antibiotics. The A673 human Ewing's sarcoma cell line (cat. no. CRL-1598) and the HOS human osteosarcoma cell line (cat. no. CRL-1543) (both from ATCC) were grown in Dulbecco's modified eagle medium supplemented with 10% fetal bovine serum and penicillin/streptomycin. The COSB canine hemangiosarcoma cell line (28 (link)) was grown in EGM-2 basal medium with the EGM-2 Bullet kit (cat. no. CC-3162; Lonza, Basel, Switzerland). The epidemiology and cancer biology of the canine cell line has been shown to extrapolate directly to humans (28 (link)).
Penicillin streptomycin antibiotics
Manufactured by American Type Culture Collection
Sourced in United States
Penicillin/streptomycin antibiotics are a combination of two commonly used antibiotics. Penicillin is a beta-lactam antibiotic that inhibits the synthesis of the bacterial cell wall, while streptomycin is an aminoglycoside antibiotic that interferes with protein synthesis. This combination is widely used in cell culture applications to prevent bacterial contamination.
Automatically generated - may contain errors
Lab products found in correlation
Mda 231, by American Type Culture Collection (2 mentions)
Mcf 7, by American Type Culture Collection (2 mentions)
Du145, by American Type Culture Collection (2 mentions)
Penicillin streptomycin, by American Type Culture Collection (2 mentions)
Lncap, by American Type Culture Collection (1 mentions)
Rpmi 1640 medium, by American Type Culture Collection (1 mentions)
Bt474, by American Type Culture Collection (1 mentions)
Mda 435, by American Type Culture Collection (1 mentions)
Mda 468, by American Type Culture Collection (1 mentions)
Mcf 10a, by American Type Culture Collection (1 mentions)
Rpmi 1640, by American Type Culture Collection (1 mentions)
Sk mel 2, by American Type Culture Collection (1 mentions)
Sk mel 5, by American Type Culture Collection (1 mentions)
A2058, by American Type Culture Collection (1 mentions)
Sk mel 28, by American Type Culture Collection (1 mentions)
Universal mycoplasma detection kit, by American Type Culture Collection (1 mentions)
4 protocols using penicillin streptomycin antibiotics
1
Sarcoma and Breast Cancer Cell Culture
2
Cell Culture Conditions for Cancer Cell Lines
3
Culturing Diverse Cancer Cell Lines
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The human breast cancer cell lines BT‐474, MCF‐7, MDA‐231, MDA‐468, the melanoma‐derived cell line, MDA‐435, and the immortalized breast epithelial cell line, MCF‐10A, were purchased from ATCC. MCF‐10A cells were grown in F12/DMEM (50/50) medium, and the rest of the cells were all cultured in RPMI medium. All culture media were supplemented with 10% (v/v) fetal bovine serum (FBS) and 1% (v/v) penicillin/streptomycin antibiotics (ATCC). The human prostate cancer cell line, DU‐145, was purchased from ATCC, and was also maintained in RPMI medium supplemented with 10% FBS and 1% penicillin/streptomycin. All the cells were maintained in a humidified atmosphere at 37°C in incubators with 5% CO2. The DU‐145 cells were chosen based on our previous studies that showed the cells to be resistant to the activity of both free and encapsulated IPA‐3.18
4
Culturing Human Melanoma Cell Lines
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The human melanoma cell lines A101D, A375, A2058, MeWo, SK-MEL-2, SK-MEL-5, SK-MEL-28 were obtained from ATCC and were cultured in RPMI-1640 containing 10% fetal bovine serum and 1% penicillin/streptomycin antibiotics (ATCC, Manassas, VA, USA) at 37°C in a humidified atmosphere of 5% CO2. Cells were sub-cultured every 2–3 days at ∼70–80% confluence. Primary adult human melanocytes were purchased from Sciencell (Carlsbad, CA, USA) and were cultured in melanocyte growth media. All melanoma cells were authenticated by STR analysis and were free of Mycoplasma infection as tested by the universal Mycoplasma detection kit (ATCC).
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