Elisa test kit

Serum creatinine levels were measured using commercial assay kits according to the manufacturer’s instructions (K625–100, Biovision, Milpitas, CA, USA). Urinary NGAL concentration was determined by a commercially available ELISA test kit (Abcam, Cambridge, MA, USA).

Sera from IgAN patients and healthy individuals were collected for the ELISA experiment. ELISA test kit was purchased from Abcam Company (United States, No. Ab108808). Experimental procedures are performed in the installation instructions.

The EJW herbs were supplied by the First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine, with authentication by Professor Sun Huifeng. In preparation, 1400 g of both Polygonatum sibiricum Redouté and Lycium chinense Mill were ground and soaked in distilled water (1:5 W/V) for 30 min, followed by extraction through boiling twice at 100°C for 1 h each time. Concentrate the mixture to achieve a density of 1.2 g mL^-1 and store at −4°C post-concentration. Donepezil (Weicai (China) Pharmaceutical Co., Ltd.), tissue fixation solution (Dalian Meilun Company), hematoxylin-eosin solution (Beijing Regen Company), methylene blue (Beijing Solarbio Company), xylene (Tianjin Fuyu Fine Chemical Co., Ltd.), ethanol (Tianjin Tianli Chemical Reagent Co., Ltd.), PBS (Germany Sigma-Aldrich Company), Anti-Aβ1-42 (Abcam, USA), ELISA test kit (Abcam, USA), DEPC (Sigma, USA), ChamQ Universal SYBR qPCR Master Mix (Nanjing Vazyme Company), HiScript II Q RT SuperMix for qPCR (+gDNA wiper) (Nanjing Vazyme Company), RIPA lysis buffer (Beijing Beyotime Company), BCA assay kit (Beijing Solarbio Company), SDS (Sigma, USA), Page ruler prestained protein ladder (Thermo Fisher Scientific, USA), anti-AGE, anti-RAGE, Anti-NF-κB, Anti-TNF alpha, Anti-Tau and GAPDH antibody (Abcam, USA).

The supernatant of spinal cord homogenate or cell culture medium was collected to detect the changes in cytokines before and after injury. In this study, Abcam's ELISA test kit was used to detect NGF, bFGF, laminin, fibronectin, IL-1β, IL-6, GFAP and CSPG levels. A microplate reader (Molecular Devices, USA) was used to read the OD value at 450 nm, and the sample concentration was calculated based on the OD value of the standard and the sample.