V atpase g1

Manufactured by Proteintech

V-ATPase G1 is a protein component of the V-type ATPase, a proton pump that plays a crucial role in regulating intracellular pH and ion homeostasis. It is involved in the acidification of various cellular compartments, such as lysosomes, secretory vesicles, and the Golgi apparatus.

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Lab products found in correlation

Vinculin, by Merck Group (2 mentions) Hoxa7, by Abcam (2 mentions) V atpase g2, by Proteintech (2 mentions) Annexin 5, by BD (1 mentions) Nh4cl, by Merck Group (1 mentions) Pou3f2, by Cell Signaling Technology (1 mentions) Syto rnaselect dyes, by Thermo Fisher Scientific (1 mentions) Bafilomycin a1 bafa1 sc 201550, by Santa Cruz Biotechnology (1 mentions) Celltrace violet, by Thermo Fisher Scientific (1 mentions) Glial fibrillary acidic protein (gfap), by Merck Group (1 mentions) Lysotracker, by Thermo Fisher Scientific (1 mentions) Olig2, by Merck Group (1 mentions) Clathrin, by Abcam (1 mentions) Fitc annexin 5 apoptosis detection kit, by BD (1 mentions) Acridine orange, by ImmunoChemistry Technologies (1 mentions) Grp78, by Cell Signaling Technology (1 mentions) Nestin, by R&D Systems (1 mentions) Calnexin, by Abcam (1 mentions) Tsg101, by Proteintech (1 mentions)

2 protocols using v atpase g1

Immunohistochemistry and Immunoblotting Protocols

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Cathepsin L (941) was from ImmunoChemistry Technologies (Bloomington, MN, USA). The following primary antibodies were used for immunohistochemistry, immunofluorescence, and immunoblotting (IB): V-ATPase G1 (16143–1-AP, Proteintech), V-ATPase V0A2 (SAB2100187, Sigma Aldrich), Vinculin (V9131, Sigma Aldrich), Ki67 (ab15580, Abcam), Podoplanin (D2–40; Ventana Medical Systems, Roche), MMP2 (10373–2-AP, Proteintech), POU3F2 (12,137, Cell Signaling), HOXA10 (TA590263, Origen), HOXA7 (ab211521, Abcam), V-ATPase G2 (25316–1-AP, Proteintech).

Terrasi A., Bertolini I., Martelli C., Gaudioso G., Di Cristofori A., Storaci A.M., Formica M., Bosari S., Caroli M., Ottobrini L., Vaccari T, & Vaira V. (2019). Specific V-ATPase expression sub-classifies IDHwt lower-grade gliomas and impacts glioma growth in vivo. EBioMedicine, 41, 214-224.

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Multiparametric Analysis of Cell Cultures

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Lysotracker, CellTrace Violet, FM lipophilic styryl (FM 1-43FX) and SYTO RNASelect dyes were from Life Technologies (Thermo Fisher Scientific). Acridine Orange was from ImmunoChemistry Technologies (Bloomington, MN, USA). PropidiumIodure (PI) and Annexin V were from BD Bioscience (FITC-Annexin V Apoptosis Detection Kit). Bafilomycin A1 (BafA1; sc-201550) was from Santa Cruz Biotechnologies whereas NH4Cl (254134) was from Sigma Aldrich.
The following primary antibodies were used for immunofluorescence, immunoblotting (IB) and flow cytometry (FACS) assays: V-ATPase G1 (16143-1-AP, Proteintech), Vinculin (V9131, Sigma Aldrich), Tsg101 (14497-1-AP, Proteintech), Ago2 (10686-1-AP, Proteintech), Clathrin (ab23440, Abcam), CD63 (sc-15363, Santa Cruz), CD9 (IB: 10626D, Thermo Fisher; FACS: 130-103-988, Miltenyi), CD81 (130-107-982, Miltenyi), Calnexin (ab31290, Abcam), Nestin (MAB 1259, R&D), Tuji (T3952, Sigma Aldrich), GFAP (G9269, Sigma Aldrich), CD11b (20991-1-AP, Proteintech), O4 (O7139, Sigma Aldrich), Olig2 (AV32753, Sigma Aldrich), CD31 (ab28364, Abcam), Vimentin (130-106-369, Miltenyi), POU3F2 (12137, Cell Signaling), HOXA10 (TA590263, Origen), HOXA7 (ab211521, Abcam), V-ATPase G2 (25316-1-AP, Proteintech), ARF6 (6ARF01, ThermoFisher Scientific), and GRP78 (3177, Cell Signaling).

Bertolini I., Terrasi A., Martelli C., Gaudioso G., Di Cristofori A., Storaci A.M., Formica M., Braidotti P., Todoerti K., Ferrero S., Caroli M., Ottobrini L., Vaccari T, & Vaira V. (2019). A GBM-like V-ATPase signature directs cell-cell tumor signaling and reprogramming via large oncosomes. EBioMedicine, 41, 225-235.

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